Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria

Resistance to antimicrobials is a growing problem of worldwide concern. Plasmids are thought to be major drivers of antibiotic resistance spread. The present work reports a simple way to recover replicative plasmids conferring antibiotic resistance from the bacteria in cheese. Purified plasmid DNA f...

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Detalles Bibliográficos
Autores: Flórez, Ana Belén, Vázquez, Lucía, Rodríguez-Carrio, Javier, Mayo Pérez, Baltasar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/263488
Acceso en línea:http://hdl.handle.net/10261/263488
Access Level:acceso abierto
Palabra clave:Antibiotic resistance
Horizontal gene transfer
Plasmids
Tetracycline and erythromycin resistance
Artisanal cheese
Lactic acid bacteria
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spelling Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteriaFlórez, Ana BelénVázquez, LucíaRodríguez-Carrio, JavierMayo Pérez, BaltasarAntibiotic resistanceHorizontal gene transferPlasmidsTetracycline and erythromycin resistanceArtisanal cheeseLactic acid bacteriaResistance to antimicrobials is a growing problem of worldwide concern. Plasmids are thought to be major drivers of antibiotic resistance spread. The present work reports a simple way to recover replicative plasmids conferring antibiotic resistance from the bacteria in cheese. Purified plasmid DNA from colonies grown in the presence of tetracycline and erythromycin was introduced into plasmid‐free strains of Lactococcus lactis, Lactiplantibacillus plantarum and Lacticaseibacillus casei. Following antibiotic selection, the plasmids from resistant transformants were isolated, analyzed by restriction enzyme digestion, and sequenced. Seven patterns were obtained for the tetracycline‐re-sistant colonies, five from L. lactis, and one each from the lactobacilli strains, as well as a single digestion profile for the erythromycin‐resistant transformants obtained in L. lactis. Sequence analysis respectively identified tet(S) and ermB in the tetracycline‐ and erythromycin‐resistance plasmids from L. lactis. No dedicated resistance genes were detected in plasmids conferring tetracycline resistance to L. casei and L. plantarum. The present results highlight the usefulness of the proposed methodology for isolating functional plasmids that confer antibiotic resistance to LAB species, widen our knowledge of antibiotic resistance in the bacteria that inhabit cheese, and emphasize the leading role of plasmids in the spread of resistance genes via the food chain.This study was supported by projects from the Spanish Ministry of Economy and Competitiveness (Ref. PID2019-110549RB-I00/AEI/10.13039/501100011033) and Asturias Principality, Spain (Ref. IDI/2018/000114). A Ph.D. grant of the Severo Ochoa Program from Asturias Principality was awarded to J.R. (BP19-098).Molecular Diversity Preservation InternationalMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Principado de AsturiasConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/263488reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110549RB-I00http://dx.doi.org/10.3390/ijms22157801Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2634882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
title Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
spellingShingle Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
Flórez, Ana Belén
Antibiotic resistance
Horizontal gene transfer
Plasmids
Tetracycline and erythromycin resistance
Artisanal cheese
Lactic acid bacteria
title_short Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
title_full Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
title_fullStr Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
title_full_unstemmed Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
title_sort Directed recovery and molecular characterization of antibiotic resistance plasmids from cheese bacteria
dc.creator.none.fl_str_mv Flórez, Ana Belén
Vázquez, Lucía
Rodríguez-Carrio, Javier
Mayo Pérez, Baltasar
author Flórez, Ana Belén
author_facet Flórez, Ana Belén
Vázquez, Lucía
Rodríguez-Carrio, Javier
Mayo Pérez, Baltasar
author_role author
author2 Vázquez, Lucía
Rodríguez-Carrio, Javier
Mayo Pérez, Baltasar
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Principado de Asturias
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antibiotic resistance
Horizontal gene transfer
Plasmids
Tetracycline and erythromycin resistance
Artisanal cheese
Lactic acid bacteria
topic Antibiotic resistance
Horizontal gene transfer
Plasmids
Tetracycline and erythromycin resistance
Artisanal cheese
Lactic acid bacteria
description Resistance to antimicrobials is a growing problem of worldwide concern. Plasmids are thought to be major drivers of antibiotic resistance spread. The present work reports a simple way to recover replicative plasmids conferring antibiotic resistance from the bacteria in cheese. Purified plasmid DNA from colonies grown in the presence of tetracycline and erythromycin was introduced into plasmid‐free strains of Lactococcus lactis, Lactiplantibacillus plantarum and Lacticaseibacillus casei. Following antibiotic selection, the plasmids from resistant transformants were isolated, analyzed by restriction enzyme digestion, and sequenced. Seven patterns were obtained for the tetracycline‐re-sistant colonies, five from L. lactis, and one each from the lactobacilli strains, as well as a single digestion profile for the erythromycin‐resistant transformants obtained in L. lactis. Sequence analysis respectively identified tet(S) and ermB in the tetracycline‐ and erythromycin‐resistance plasmids from L. lactis. No dedicated resistance genes were detected in plasmids conferring tetracycline resistance to L. casei and L. plantarum. The present results highlight the usefulness of the proposed methodology for isolating functional plasmids that confer antibiotic resistance to LAB species, widen our knowledge of antibiotic resistance in the bacteria that inhabit cheese, and emphasize the leading role of plasmids in the spread of resistance genes via the food chain.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/263488
url http://hdl.handle.net/10261/263488
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110549RB-I00
http://dx.doi.org/10.3390/ijms22157801
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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