Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study

Abstract The objective of this study was to analyze the in vitro stability and toxicity of liposomes containing guarana in skin cell lines. The liposomes were produced by the reverse phase evaporation method containing 1 mg/mL guarana. The stability of the liposomes was evaluated by physical-chemica...

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Autores: Roggia, Isabel, Gomes, Patrícia, Dalcin, Ana Júlia F., Ourique, Aline F., Cruz, Ivana B.M. da, Ribeiro, Euler E., Mitjans Arnal, Montserrat, Vinardell Martínez-Hidalgo, Ma. Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/218194
Acceso en línea:https://hdl.handle.net/2445/218194
Access Level:acceso abierto
Palabra clave:Estimulants
Liposomes
Plantes tropicals
Stimulants
Tropical plants
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spelling Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro StudyRoggia, IsabelGomes, PatríciaDalcin, Ana Júlia F.Ourique, Aline F.Cruz, Ivana B.M. daRibeiro, Euler E.Mitjans Arnal, MontserratVinardell Martínez-Hidalgo, Ma. PilarEstimulantsLiposomesPlantes tropicalsStimulantsLiposomesTropical plantsAbstract The objective of this study was to analyze the in vitro stability and toxicity of liposomes containing guarana in skin cell lines. The liposomes were produced by the reverse phase evaporation method containing 1 mg/mL guarana. The stability of the liposomes was evaluated by physical-chemical parameters for up to 90 days using three different storage conditions. The cytotoxicity of guarana (GL), liposomes (B-Lip), and guarana-loaded liposomes (G-Lip) was evaluated on spontaneously immortalized human keratinocyte cell lines (HaCaT), murine Swiss albino fibroblasts (3T3), and human fibroblasts (1BR.3.G). The evaluation was performed using cellular viability analysis. The techniques used were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red capturing (NRU), and the analyses were conducted after 24, 48, and 72 h of exposure of these cells to the different treatments. The G-Lip exhibited physical-chemical stability for 60 days when the samples were stored in a refrigerator. The GL, B-Lip, and G-Lip demonstrated low cytotoxicity in the three different cell cultures tested since a small reduction in cell viability was only observed at the highest concentrations. In addition, greater cell damage was observed for B-Lip; however, guarana protected the cells from this damage. Thus, G-Lip structures can be considered promising systems for topical applications. Keywords: Paullinia cupana; natural products; nanoparticles; stability; cytotoxicity; cell cultureMDPI2025202520232025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1 p.application/pdfhttps://hdl.handle.net/2445/218194Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/cosmetics10030079Cosmetics, 2023, vol. 10, num.3https://doi.org/10.3390/cosmetics10030079cc-by (c) Roggia, I. et al., 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2181942026-05-29T05:05:01Z
dc.title.none.fl_str_mv Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
title Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
spellingShingle Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
Roggia, Isabel
Estimulants
Liposomes
Plantes tropicals
Stimulants
Liposomes
Tropical plants
title_short Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
title_full Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
title_fullStr Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
title_full_unstemmed Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
title_sort Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study
dc.creator.none.fl_str_mv Roggia, Isabel
Gomes, Patrícia
Dalcin, Ana Júlia F.
Ourique, Aline F.
Cruz, Ivana B.M. da
Ribeiro, Euler E.
Mitjans Arnal, Montserrat
Vinardell Martínez-Hidalgo, Ma. Pilar
author Roggia, Isabel
author_facet Roggia, Isabel
Gomes, Patrícia
Dalcin, Ana Júlia F.
Ourique, Aline F.
Cruz, Ivana B.M. da
Ribeiro, Euler E.
Mitjans Arnal, Montserrat
Vinardell Martínez-Hidalgo, Ma. Pilar
author_role author
author2 Gomes, Patrícia
Dalcin, Ana Júlia F.
Ourique, Aline F.
Cruz, Ivana B.M. da
Ribeiro, Euler E.
Mitjans Arnal, Montserrat
Vinardell Martínez-Hidalgo, Ma. Pilar
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estimulants
Liposomes
Plantes tropicals
Stimulants
Liposomes
Tropical plants
topic Estimulants
Liposomes
Plantes tropicals
Stimulants
Liposomes
Tropical plants
description Abstract The objective of this study was to analyze the in vitro stability and toxicity of liposomes containing guarana in skin cell lines. The liposomes were produced by the reverse phase evaporation method containing 1 mg/mL guarana. The stability of the liposomes was evaluated by physical-chemical parameters for up to 90 days using three different storage conditions. The cytotoxicity of guarana (GL), liposomes (B-Lip), and guarana-loaded liposomes (G-Lip) was evaluated on spontaneously immortalized human keratinocyte cell lines (HaCaT), murine Swiss albino fibroblasts (3T3), and human fibroblasts (1BR.3.G). The evaluation was performed using cellular viability analysis. The techniques used were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red capturing (NRU), and the analyses were conducted after 24, 48, and 72 h of exposure of these cells to the different treatments. The G-Lip exhibited physical-chemical stability for 60 days when the samples were stored in a refrigerator. The GL, B-Lip, and G-Lip demonstrated low cytotoxicity in the three different cell cultures tested since a small reduction in cell viability was only observed at the highest concentrations. In addition, greater cell damage was observed for B-Lip; however, guarana protected the cells from this damage. Thus, G-Lip structures can be considered promising systems for topical applications. Keywords: Paullinia cupana; natural products; nanoparticles; stability; cytotoxicity; cell culture
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/218194
url https://hdl.handle.net/2445/218194
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cosmetics10030079
Cosmetics, 2023, vol. 10, num.3
https://doi.org/10.3390/cosmetics10030079
dc.rights.none.fl_str_mv cc-by (c) Roggia, I. et al., 2023
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Roggia, I. et al., 2023
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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