Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis

The incorporation of lactic acid bacteria (LAB) in edible films and coatings has recently emerged as an innovative strategy to provide packaging films with new functionalities in order to ensure food quality and safety. In this work, bioactive films were made by incorporating Lactococcus lactis subs...

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Detalles Bibliográficos
Autores: Settier Ramírez, Laura, López-Carballo, Gracia, Gavara, Rafael, Hernández-Muñoz, Pilar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/386457
Acceso en línea:http://hdl.handle.net/10261/386457
https://api.elsevier.com/content/abstract/scopus_id/85053050598
Access Level:acceso abierto
Palabra clave:Gelatin
Lactococcus lactis
Listeria monocytogenes
Protein hydrolysates
PVOH films
Sodium caseinate
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spelling Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactisSettier Ramírez, LauraLópez-Carballo, GraciaGavara, RafaelHernández-Muñoz, PilarGelatinLactococcus lactisListeria monocytogenesProtein hydrolysatesPVOH filmsSodium caseinateThe incorporation of lactic acid bacteria (LAB) in edible films and coatings has recently emerged as an innovative strategy to provide packaging films with new functionalities in order to ensure food quality and safety. In this work, bioactive films were made by incorporating Lactococcus lactis subsp. lactis in cast polyvinyl alcohol (PVOH) matrices, alone or blended with a small percentage of proteins, protein hydrolysates, or yeast extract, and the effect of incorporating them on the morphology and optical properties of the PVOH films was studied. Moreover, the viability of L. lactis in the films developed stored at 20 °C and 43.2% relative humidity for four weeks, and the antimicrobial activity against L. monocytogenes were determined. The thickness, color properties, and morphology of the films incorporating LAB and nutrients did not show significant differences compared with plain PVOH films, but moisture content increased slightly with nutrient incorporation. L. lactis remained viable for 4 weeks of storage, but viability depended on the matrix composition, being lower in plain PVOH films and higher in films supplemented with proteins, hydrolysates, or yeast extract. The highest antimicrobial activity was observed in PVOH matrices with hydrolyzed gelatin or casein, and the lowest in plain PVOH films. The growth of L. lactis was similar in all the films after incubation in contact with L. monocytogenes at 37 °C in liquid medium, regardless of the initial concentration. The films developed could be applied in the design of food packages with the purpose of inhibiting growth of L. monocytogenes.The authors acknowledge the financial support of the Spanish Ministry of Economy and Competitiveness (AGL2015-64595-R).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Gavara, Rafael [0000-0001-6376-8939]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/386457https://api.elsevier.com/content/abstract/scopus_id/85053050598reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/Ministerio de Economía y Competitividad/Plan Estatal de Investigación Científica y Técnica y de Innovación2013-2016/AGL2015-64595-RFood Hydrocolloidshttps://doi.org/10.1016/j.foodhyd.2018.08.007Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3864572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
title Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
spellingShingle Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
Settier Ramírez, Laura
Gelatin
Lactococcus lactis
Listeria monocytogenes
Protein hydrolysates
PVOH films
Sodium caseinate
title_short Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
title_full Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
title_fullStr Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
title_full_unstemmed Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
title_sort Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis
dc.creator.none.fl_str_mv Settier Ramírez, Laura
López-Carballo, Gracia
Gavara, Rafael
Hernández-Muñoz, Pilar
author Settier Ramírez, Laura
author_facet Settier Ramírez, Laura
López-Carballo, Gracia
Gavara, Rafael
Hernández-Muñoz, Pilar
author_role author
author2 López-Carballo, Gracia
Gavara, Rafael
Hernández-Muñoz, Pilar
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Gavara, Rafael [0000-0001-6376-8939]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gelatin
Lactococcus lactis
Listeria monocytogenes
Protein hydrolysates
PVOH films
Sodium caseinate
topic Gelatin
Lactococcus lactis
Listeria monocytogenes
Protein hydrolysates
PVOH films
Sodium caseinate
description The incorporation of lactic acid bacteria (LAB) in edible films and coatings has recently emerged as an innovative strategy to provide packaging films with new functionalities in order to ensure food quality and safety. In this work, bioactive films were made by incorporating Lactococcus lactis subsp. lactis in cast polyvinyl alcohol (PVOH) matrices, alone or blended with a small percentage of proteins, protein hydrolysates, or yeast extract, and the effect of incorporating them on the morphology and optical properties of the PVOH films was studied. Moreover, the viability of L. lactis in the films developed stored at 20 °C and 43.2% relative humidity for four weeks, and the antimicrobial activity against L. monocytogenes were determined. The thickness, color properties, and morphology of the films incorporating LAB and nutrients did not show significant differences compared with plain PVOH films, but moisture content increased slightly with nutrient incorporation. L. lactis remained viable for 4 weeks of storage, but viability depended on the matrix composition, being lower in plain PVOH films and higher in films supplemented with proteins, hydrolysates, or yeast extract. The highest antimicrobial activity was observed in PVOH matrices with hydrolyzed gelatin or casein, and the lowest in plain PVOH films. The growth of L. lactis was similar in all the films after incubation in contact with L. monocytogenes at 37 °C in liquid medium, regardless of the initial concentration. The films developed could be applied in the design of food packages with the purpose of inhibiting growth of L. monocytogenes.
publishDate 2018
dc.date.none.fl_str_mv 2018
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/386457
https://api.elsevier.com/content/abstract/scopus_id/85053050598
url http://hdl.handle.net/10261/386457
https://api.elsevier.com/content/abstract/scopus_id/85053050598
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/Ministerio de Economía y Competitividad/Plan Estatal de Investigación Científica y Técnica y de Innovación2013-2016/AGL2015-64595-R
Food Hydrocolloids
https://doi.org/10.1016/j.foodhyd.2018.08.007
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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