Microbiological risk assessment of Turkey and chicken meat for consumer: Significant differences regarding multidrug resistance, mcr or presence of hybrid aEPEC/ExPEC pathotypes of E. coli
To assess the microbiological risk for consumers, we propose a lab workflow based on six virulence/antimicrobial resistance (AMR) traits, and including a duplex PCR for the screening of extraintestinal pathogenic E. coli (ExPEC). This protocol was tested in 100 poultry meat products. The characteriz...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de Santiago de Compostela (USC) |
| Repositorio: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| Idioma: | inglés |
| OAI Identifier: | oai:minerva.usc.gal:10347/44583 |
| Acceso en línea: | https://hdl.handle.net/10347/44583 |
| Access Level: | acceso abierto |
| Palabra clave: | Escherichia coli Antimicrobial resistance (AMR) mcr-1 ESBL ExPEC ST131 Poultry meat Risk assessment One-health |
| Sumario: | To assess the microbiological risk for consumers, we propose a lab workflow based on six virulence/antimicrobial resistance (AMR) traits, and including a duplex PCR for the screening of extraintestinal pathogenic E. coli (ExPEC). This protocol was tested in 100 poultry meat products. The characterization of 323 isolates revealed that poultry meat is a rich phylogenetic source of E. coli phylogroups (A to G) and Escherichia clade I. Non-susceptible E. coli isolates to monobactams, 3rd-generation cephalosporins and/or fluoroquinolones, were present in 71% of the samples. Besides, 47% carried ≥2 different E. coli positive for ESBL, pAmpC or mcr genes. Isolates from 78% of the poultry meat exhibited ExPEC status, and 53% were carriers of isolates positive for the uropathogenic (UPEC) status. The sequence types (STs) identified in 86% of the samples belonged to the so-called ExPEC high-risk lineages, being 73% carriers of clonal groups identified in human infections of the same Health Area. Moreover, different human-associated clones co-occurred in same meat sample: ST131-B2 (CH40-22), ST648-F (CH4-58), ST93-A (CH11-neg) or ST95-B2 (CH38-27), ST354-F (CH88-58), ST155-B1 (CH4-neg). Globally, 84% of the meat samples posed ≥3 risks, including resistance genes, successful clones and virulence traits. Turkey meat showed significant higher rates concerning mcr-carriage or multidrug resistance; while the ExPEC status rate, or the presence of hybrid pathotypes such as the aEPEC/ExPEC O153:H10-A-ST10 (CH11-54), were associated with chicken origin (P < 0.05). In a “Farm to Fork Strategy”, ExPEC should be clearly included in food surveillance. |
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