Dendritic cells delivered inside human carcinomas are sequestered by interleukin-8

In the course of a clinical trial consisting of intratumoral injections of dendritic cells (DCs) transfected to produce interleukin-12, the use of (111)In-labeled tracing doses of DCs showed that most DCs remained inside tumor tissue, instead of migrating out. In search for factors that could explai...

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Detalles Bibliográficos
Autores: Feijoo-Blanco, E. (Esperanza)|||/items/a4066dcd-ee21-4cb4-8eb0-1fa574e501b0, Alfaro, C. (Carlos)|||/items/6543acf2-b8b2-46cc-b8ed-884af7fcf8bd, Mazzolini, G. (Guillermo)|||/items/73a250e4-d50b-45d7-bf6b-f222192f702e, Serra, P. (Patricia)|||/items/162fbb33-2ab8-4c67-8482-e583b3277e6a, Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4, Arina, A. (Ainhoa)|||/items/6d57bce6-8d5c-4f16-8069-c37817732576, Huarte, E. (Eduardo)|||/items/c539fbb4-0e58-4af3-8379-6d8422060082, Tirapu, I. (Íñigo)|||/items/c563bf91-df49-4b18-b715-76b1b014d0e0, Palencia-Coto, B. (Belén)|||/items/c439a2a6-fad9-4a0b-8309-ceca8c25edf2, Murillo-Sauca, O. (Oihana)|||/items/f9d83912-f50c-4f77-ab5b-cb1c916d5f89, Ruiz, J. (Juan)|||/items/e71ee758-aef7-4ed6-b282-e483e9d9ba8e, Sangro-Gómez-Acebo, B.C. (Bruno Carlos)|||/items/594bbdbb-046a-4ab2-878c-cb4fe577af49, Richter, J.A. (José Ángel)|||/items/4b21b403-ba6f-4220-8493-3453c8f876c3, Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71, Melero, I. (Ignacio)|||/items/82113ea8-7ce1-49d5-9ee3-42cf20db1c4e
Tipo de recurso: artículo
Fecha de publicación:2005
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/20414
Acceso en línea:https://hdl.handle.net/10171/20414
Access Level:acceso abierto
Palabra clave:IL-8
Dendritic cells
Immunotherapy
Chemotaxis
Cancer
Descripción
Sumario:In the course of a clinical trial consisting of intratumoral injections of dendritic cells (DCs) transfected to produce interleukin-12, the use of (111)In-labeled tracing doses of DCs showed that most DCs remained inside tumor tissue, instead of migrating out. In search for factors that could explain this retention, it was found that tumors from patients suffering hepatocellular carcinoma, colorectal or pancreatic cancer were producing IL-8 and that this chemokine attracted monocyte-derived dendritic cells that uniformly express both IL-8 receptors CXCR1 and CXCR2. Accordingly, neutralizing antihuman IL-8 monoclonal antibodies blocked the chemotactic attraction of DCs by recombinant IL-8, as well as by the serum of the patients or culture supernatants of human colorectal carcinomas. In addition, tissue culture supernatants of colon carcinoma cells inhibited DC migration induced by MIP-3beta in an IL-8-dependent fashion. IL-8 production in malignant tissue and the responsiveness of DCs to IL-8 are a likely explanation of the clinical images, which suggest retention of DCs inside human malignant lesions. Impairment of DC migration toward lymphoid tissue could be involved in cancer immune evasion.

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