Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean

16 pages, 7 figures, 3 tables

Detalles Bibliográficos
Autores: Mendes, Maria Cristina de Q., Nunes, José Marcos C., Fraga, Santiago, Rodríguez Hernández, Francisco José, Franco, José Mariano, Riobó, Pilar, Branco, Suema, Menezes, Mariângela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/200764
Acceso en línea:http://hdl.handle.net/10261/200764
Access Level:acceso abierto
Palabra clave:Coolia malayensis
Coolia tropicalis
Hemolytic assay
LC-HRMS
Prorocentrum emarginatum
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spelling Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic OceanMendes, Maria Cristina de Q.Nunes, José Marcos C.Fraga, SantiagoRodríguez Hernández, Francisco JoséFranco, José MarianoRiobó, PilarBranco, SuemaMenezes, MariângelaCoolia malayensisCoolia tropicalisHemolytic assayLC-HRMSProrocentrum emarginatum16 pages, 7 figures, 3 tablesThe morphology, molecular phylogeny and toxinology of two Coolia and one Prorocentrum dinoflagellate strains from Brazil were characterized. They matched with Coolia malayensis and Coolia tropicalis morphotypes, while the Prorocentrum strain fitted well with the morphology of Prorocentrum emarginatum. Complementary identification by molecular analyses was carried out based on LSU and ITS-5.8S rDNA. Phylogenetic analyses of Coolia strains (D1/D2 region, LSU rDNA), showed that C. malayensis (strain UFBA044) segregated together with sequences of this species from other parts of the world, but diverged earlier in a separate branch to sequences from São Paulo (Brazil) or Caribbean areas. Coolia tropicalis (strain UFBA055) grouped with other sequences of this species, in a subclade with an isolate from Belize, closer to a subgroup including isolates from Thailand, Australia and Hong Kong. Phylogenetic analyses (ITS-5.8S rDNA) of P. emarginatum (strain UFBA033) grouped together with another sequence of this species from China Sea. Diarrhetic shellfish poisoning toxins (OA, DTXs and PTX2) were not detected in P. emarginatum by mass spectrometry analyses. However, hemolytic assays in P. emarginatum and both Coolia strains in this study showed positive resultsThis study was funded by projects FAPESB – RED0006/2012 and CNPq (Productivity Scholarship in Research # 07368/2015-7), which were granted to J.M.C. Nunes; another CNPq grant (#471303/2013-5) was granted to M. Menezes; the scholarships from CAPES (PhD scholarship #9947-14-14-0/2014) and FAPESB (PhD scholarship Bol # 10685/2013) were granted to M.C.Q. MendesPeer reviewedWalter de GruyterFundação de Amparo à Pesquisa do Estado da BahiaConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/200764reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1515/bot-2018-0053Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2007642026-05-22T06:33:51Z
dc.title.none.fl_str_mv Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
title Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
spellingShingle Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
Mendes, Maria Cristina de Q.
Coolia malayensis
Coolia tropicalis
Hemolytic assay
LC-HRMS
Prorocentrum emarginatum
title_short Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
title_full Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
title_fullStr Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
title_full_unstemmed Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
title_sort Morphology, molecular phylogeny and toxinology of Coolia and Prorocentrum strains isolated from the tropical South Western Atlantic Ocean
dc.creator.none.fl_str_mv Mendes, Maria Cristina de Q.
Nunes, José Marcos C.
Fraga, Santiago
Rodríguez Hernández, Francisco José
Franco, José Mariano
Riobó, Pilar
Branco, Suema
Menezes, Mariângela
author Mendes, Maria Cristina de Q.
author_facet Mendes, Maria Cristina de Q.
Nunes, José Marcos C.
Fraga, Santiago
Rodríguez Hernández, Francisco José
Franco, José Mariano
Riobó, Pilar
Branco, Suema
Menezes, Mariângela
author_role author
author2 Nunes, José Marcos C.
Fraga, Santiago
Rodríguez Hernández, Francisco José
Franco, José Mariano
Riobó, Pilar
Branco, Suema
Menezes, Mariângela
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação de Amparo à Pesquisa do Estado da Bahia
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Coolia malayensis
Coolia tropicalis
Hemolytic assay
LC-HRMS
Prorocentrum emarginatum
topic Coolia malayensis
Coolia tropicalis
Hemolytic assay
LC-HRMS
Prorocentrum emarginatum
description 16 pages, 7 figures, 3 tables
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/200764
url http://hdl.handle.net/10261/200764
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1515/bot-2018-0053
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Walter de Gruyter
publisher.none.fl_str_mv Walter de Gruyter
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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