Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption

The objective of this study was to investigate the effect of complexation of epigallocatechin-gallate (EGCG) to a caseinate-stabilized oil-water interface on the bioefficacy of EGCG. The bioaccessibility, anti-proliferative activity, and intestinal uptake of EGCG are reported. Higher amount of EGCG...

Descripción completa

Detalles Bibliográficos
Autores: Sabouri, Somayeh, Arranz, Elena, Guri, Anilda, Corredig, Milena
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/383958
Acceso en línea:http://hdl.handle.net/10261/383958
Access Level:acceso abierto
Palabra clave:Tea polyphenols
Sodium caseinate emulsions
Bioaccessibility
Intestinal absorption
id ES_bf9ee8069ff5638e9652f4d533fbbef2
oai_identifier_str oai:digital.csic.es:10261/383958
network_acronym_str ES
network_name_str España
repository_id_str
spelling Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorptionSabouri, SomayehArranz, ElenaGuri, AnildaCorredig, MilenaTea polyphenolsSodium caseinate emulsionsBioaccessibilityIntestinal absorptionThe objective of this study was to investigate the effect of complexation of epigallocatechin-gallate (EGCG) to a caseinate-stabilized oil-water interface on the bioefficacy of EGCG. The bioaccessibility, anti-proliferative activity, and intestinal uptake of EGCG are reported. Higher amount of EGCG was present in digested emulsions compared to their equivalent solutions after digestion. The incorporation of EGCG in sodium caseinate emulsions improved its bioaccesibility and this was confirmed by cytotoxicity assays on Caco-2 cells. Intestinal absorption of EGCG was also studied with in vitro transport experiments. It was difficult to obtain a quantitative measurement of the EGCG in the basolateral fraction, but the fractions showed a clear anti-proliferative activity. These results would suggest the use of a cytotoxicity assay on cell cultures to estimate the extent of intestinal absorption of the bioactive catechin EGCG. The findings demonstrated that sodium caseinate-stabilized emulsions can be used as a platform for delivery of EGCG.This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC). The authors thank DSM Nutritional Products (Ayr, Ontario, Canada) for donating the green tea extract. Authors participate in the EU COST Action INFOGEST. Also authors would like to thank Dr. Tracey Campbell at the mass spectrometry facility of Centre for Microbial Chemical Biology at McMaster University.Peer reviewedElsevierNatural Sciences and Engineering Research Council of CanadaEuropean Commission202520252018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionapplication/pdfhttp://hdl.handle.net/10261/383958reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.jff.2018.03.009Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3839582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
title Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
spellingShingle Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
Sabouri, Somayeh
Tea polyphenols
Sodium caseinate emulsions
Bioaccessibility
Intestinal absorption
title_short Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
title_full Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
title_fullStr Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
title_full_unstemmed Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
title_sort Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption
dc.creator.none.fl_str_mv Sabouri, Somayeh
Arranz, Elena
Guri, Anilda
Corredig, Milena
author Sabouri, Somayeh
author_facet Sabouri, Somayeh
Arranz, Elena
Guri, Anilda
Corredig, Milena
author_role author
author2 Arranz, Elena
Guri, Anilda
Corredig, Milena
author2_role author
author
author
dc.contributor.none.fl_str_mv Natural Sciences and Engineering Research Council of Canada
European Commission
dc.subject.none.fl_str_mv Tea polyphenols
Sodium caseinate emulsions
Bioaccessibility
Intestinal absorption
topic Tea polyphenols
Sodium caseinate emulsions
Bioaccessibility
Intestinal absorption
description The objective of this study was to investigate the effect of complexation of epigallocatechin-gallate (EGCG) to a caseinate-stabilized oil-water interface on the bioefficacy of EGCG. The bioaccessibility, anti-proliferative activity, and intestinal uptake of EGCG are reported. Higher amount of EGCG was present in digested emulsions compared to their equivalent solutions after digestion. The incorporation of EGCG in sodium caseinate emulsions improved its bioaccesibility and this was confirmed by cytotoxicity assays on Caco-2 cells. Intestinal absorption of EGCG was also studied with in vitro transport experiments. It was difficult to obtain a quantitative measurement of the EGCG in the basolateral fraction, but the fractions showed a clear anti-proliferative activity. These results would suggest the use of a cytotoxicity assay on cell cultures to estimate the extent of intestinal absorption of the bioactive catechin EGCG. The findings demonstrated that sodium caseinate-stabilized emulsions can be used as a platform for delivery of EGCG.
publishDate 2018
dc.date.none.fl_str_mv 2018
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/383958
url http://hdl.handle.net/10261/383958
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.jff.2018.03.009
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418407536885760
score 15.811543

Similares en LA Referencia