Implant surface physicochemistry affects keratinocyte hemidesmosome formation
Previous studies have shown hydrophilic/hydrophobic implant surfaces stimulate/hinder osseointegration. An analogous concept was applied here using common bio-logical functional groups on a model surface to promote oral keratinocytes (OKs) pro-liferation and hemidesmosomes (HD) to extend implant lif...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/421981 |
| Acceso en línea: | https://hdl.handle.net/2117/421981 https://dx.doi.org/10.1002/jbm.a.37486 |
| Access Level: | acceso abierto |
| Palabra clave: | Attachment Hemidesmosome Implant Keratinocyte Organosilane Physicochemistry Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials::Implants artificials |
| Sumario: | Previous studies have shown hydrophilic/hydrophobic implant surfaces stimulate/hinder osseointegration. An analogous concept was applied here using common bio-logical functional groups on a model surface to promote oral keratinocytes (OKs) pro-liferation and hemidesmosomes (HD) to extend implant lifespans through increasedsoft tissue attachment. However, it is unclear what physicochemistry stimulates HDs.Thus, common biological functional groups ( NH2 , OH, and CH3) were functiona-lized on glass using silanization. Non-functionalized plasma-cleaned glass and Hsilanization were controls. Surface modifications were confirmed with X-ray photo-electron spectroscopy and water contact angle. The amount of bovine serum albumin(BSA) and fibrinogen, and BSA thickness, were assessed to understand how adsorbedprotein properties were influenced by physicochemistry and may influence HDs. OKsproliferation was measured, and HDs were quantified with immunofluorescence forcollagen XVII and integrin ß4. Plasma-cleaned surfaces were the most hydrophilicgroup overall, while CH 3 was the most hydrophobic and OH was the most hydro-philic among functionalized groups. Modification with the OH chemical groupshowed the highest OKs proliferation and HD expression. The OKs response onOH surfaces appeared to not correlate to the amount or thickness of adsorbedmodel proteins. These results reveal relevant surface physicochemical features tofavor HDs and improve implant soft tissue attachment. |
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