The 40 S -LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity

Ribosomes execute the transcriptional program in every cell. Critical to sustain nearly all cellular activities, ribosome biogenesis requires the translation of ~200 factors of which 80 are ribosomal proteins (RPs). As ribosome synthesis depends on RP mRNA translation, a priority within the translat...

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Detalles Bibliográficos
Autores: Fuentes, Pedro, Pelletier, Joffrey, Martinez Herráez, Carolina, Diez Obrero, Virginia, Iannizzotto, Flavia, Rubio, Teresa, Garcia Cajide, Marta, Menoyo, Sandra, Moreno Aguado, Víctor, Salazar, Ramón, Tauler Girona, Albert, Gentilella, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/181680
Acceso en línea:https://hdl.handle.net/2445/181680
Access Level:acceso abierto
Palabra clave:Ribosomes
Síntesi proteica
Protein synthesis
Descripción
Sumario:Ribosomes execute the transcriptional program in every cell. Critical to sustain nearly all cellular activities, ribosome biogenesis requires the translation of ~200 factors of which 80 are ribosomal proteins (RPs). As ribosome synthesis depends on RP mRNA translation, a priority within the translatome architecture should exist to ensure the preservation of ribosome biogenesis capacity, particularly under adverse growth conditions. Here, we show that under critical metabolic constraints characterized by mTOR inhibition, LARP1 complexed with the 40S subunit protects from ribophagy the mRNAs regulon for ribosome biogenesis and protein synthesis, acutely preparing the translatome to promptly resume ribosomes production after growth conditions return permissive. Characterizing the LARP1-protected translatome revealed a set of 5′TOP transcript isoforms other than RPs involved in energy production and in mitochondrial function, among other processes, indicating that the mTOR-LARP1-5′TOP axis acts at the translational level as a primary guardian of the cellular anabolic capacity.

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