Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages
Background: Corpora amylacea of human brain, recently renamed as wasteosomes, are granular structures that appear during aging and also accumulate in specific areas of the brain in neurodegenerative conditions. Acting as waste containers, wasteosomes are formed by polyglucosan aggregates that entrap...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/217855 |
| Acceso en línea: | https://hdl.handle.net/2445/217855 |
| Access Level: | acceso abierto |
| Palabra clave: | Envelliment cerebral Malalties neurodegeneratives Immunitat Aging brain Neurodegenerative Diseases Immunity |
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Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophagesRiba Baqués, MartaCampo Sabariz, JoanTena, IraidaMolina Porcel, LauraXimelis, TeresaCalvo Ademuz, MariaFerrer i Roig, RuthMartín Venegas, RaquelValle i Macià, Jaume delVilaplana i Hortensi, JordiPelegrí i Gabaldà, CarmeEnvelliment cerebralMalalties neurodegenerativesImmunitatAging brainNeurodegenerative DiseasesImmunityBackground: Corpora amylacea of human brain, recently renamed as wasteosomes, are granular structures that appear during aging and also accumulate in specific areas of the brain in neurodegenerative conditions. Acting as waste containers, wasteosomes are formed by polyglucosan aggregates that entrap and isolate toxic and waste substances of different origins. They are expelled from the brain to the cerebrospinal fluid (CSF), and can be phagocytosed by macrophages. In the present study, we analyze the phagocytosis of wasteosomes and the mechanisms involved in this process. Accordingly, we purified wasteosomes from post-mortem extracted human CSF and incubated them with THP-1 macrophages. Immunofluorescence staining and time-lapse recording techniques were performed to evaluate the phagocytosis. We also immunostained human hippocampal sections to study possible interactions between wasteosomes and macrophages at central nervous system interfaces. Results: We observed that the wasteosomes obtained from post-mortem extracted CSF are opsonized by MBL and the C3b complement protein. Moreover, we observed that CD206 and CD35 receptors may be involved in the phagocytosis of these wasteosomes by THP-1 macrophages. Once phagocytosed, wasteosomes become degraded and some of the resulting fractions can be exposed on the surface of macrophages and interchanged between different macrophages. However, brain tissue studies show that, in physiological conditions, CD206 but not CD35 receptors may be involved in the phagocytosis of wasteosomes. Conclusions: The present study indicates that macrophages have the machinery required to process and degrade wasteosomes, and that macrophages can interact in different ways with wasteosomes. In physiological conditions, the main mechanism involve CD206 receptors and M2 macrophages, which trigger the phagocytosis of wasteosomes without inducing inflammatory responses, thus avoiding tissue damage. However, altered wasteosomes like those obtained from post-mortem extracted CSF, which may exhibit waste elements, become opsonized by MBL and C3b, and so CD35 receptors constitute another possible mechanism of phagocytosis, leading in this case to inflammatory responses. Keywords: Brain; C3b; CD206; CD35; Corpora amylacea; IgM; Natural immunity; Phagocytosis; Wasteosome.BioMed Central2025202520222025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion17 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/217855Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a:Cell & Bioscience, 2022, vol. 12, num.177, p. 1-17cc-by (c) Riba, M. et al., 2022http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2178552026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages |
| title |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages |
| spellingShingle |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages Riba Baqués, Marta Envelliment cerebral Malalties neurodegeneratives Immunitat Aging brain Neurodegenerative Diseases Immunity |
| title_short |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages |
| title_full |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages |
| title_fullStr |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages |
| title_full_unstemmed |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages |
| title_sort |
Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages |
| dc.creator.none.fl_str_mv |
Riba Baqués, Marta Campo Sabariz, Joan Tena, Iraida Molina Porcel, Laura Ximelis, Teresa Calvo Ademuz, Maria Ferrer i Roig, Ruth Martín Venegas, Raquel Valle i Macià, Jaume del Vilaplana i Hortensi, Jordi Pelegrí i Gabaldà, Carme |
| author |
Riba Baqués, Marta |
| author_facet |
Riba Baqués, Marta Campo Sabariz, Joan Tena, Iraida Molina Porcel, Laura Ximelis, Teresa Calvo Ademuz, Maria Ferrer i Roig, Ruth Martín Venegas, Raquel Valle i Macià, Jaume del Vilaplana i Hortensi, Jordi Pelegrí i Gabaldà, Carme |
| author_role |
author |
| author2 |
Campo Sabariz, Joan Tena, Iraida Molina Porcel, Laura Ximelis, Teresa Calvo Ademuz, Maria Ferrer i Roig, Ruth Martín Venegas, Raquel Valle i Macià, Jaume del Vilaplana i Hortensi, Jordi Pelegrí i Gabaldà, Carme |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Envelliment cerebral Malalties neurodegeneratives Immunitat Aging brain Neurodegenerative Diseases Immunity |
| topic |
Envelliment cerebral Malalties neurodegeneratives Immunitat Aging brain Neurodegenerative Diseases Immunity |
| description |
Background: Corpora amylacea of human brain, recently renamed as wasteosomes, are granular structures that appear during aging and also accumulate in specific areas of the brain in neurodegenerative conditions. Acting as waste containers, wasteosomes are formed by polyglucosan aggregates that entrap and isolate toxic and waste substances of different origins. They are expelled from the brain to the cerebrospinal fluid (CSF), and can be phagocytosed by macrophages. In the present study, we analyze the phagocytosis of wasteosomes and the mechanisms involved in this process. Accordingly, we purified wasteosomes from post-mortem extracted human CSF and incubated them with THP-1 macrophages. Immunofluorescence staining and time-lapse recording techniques were performed to evaluate the phagocytosis. We also immunostained human hippocampal sections to study possible interactions between wasteosomes and macrophages at central nervous system interfaces. Results: We observed that the wasteosomes obtained from post-mortem extracted CSF are opsonized by MBL and the C3b complement protein. Moreover, we observed that CD206 and CD35 receptors may be involved in the phagocytosis of these wasteosomes by THP-1 macrophages. Once phagocytosed, wasteosomes become degraded and some of the resulting fractions can be exposed on the surface of macrophages and interchanged between different macrophages. However, brain tissue studies show that, in physiological conditions, CD206 but not CD35 receptors may be involved in the phagocytosis of wasteosomes. Conclusions: The present study indicates that macrophages have the machinery required to process and degrade wasteosomes, and that macrophages can interact in different ways with wasteosomes. In physiological conditions, the main mechanism involve CD206 receptors and M2 macrophages, which trigger the phagocytosis of wasteosomes without inducing inflammatory responses, thus avoiding tissue damage. However, altered wasteosomes like those obtained from post-mortem extracted CSF, which may exhibit waste elements, become opsonized by MBL and C3b, and so CD35 receptors constitute another possible mechanism of phagocytosis, leading in this case to inflammatory responses. Keywords: Brain; C3b; CD206; CD35; Corpora amylacea; IgM; Natural immunity; Phagocytosis; Wasteosome. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2025 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/217855 |
| url |
https://hdl.handle.net/2445/217855 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: Cell & Bioscience, 2022, vol. 12, num.177, p. 1-17 |
| dc.rights.none.fl_str_mv |
cc-by (c) Riba, M. et al., 2022 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Riba, M. et al., 2022 http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
17 p. application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Bioquímica i Fisiologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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