Insights into crucial molecules and protein channels involved in pig sperm cryopreservation
Cryopreservation is the most efficient procedure for long-term preservation of mammalian sperm; however, its use is not currently dominant for boar sperm before its use for artificial insemination. In fact, freezing and thawing have an extensive detrimental effect on sperm function and lead to impai...
| Autor: | |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10256/25116 |
| Acceso en línea: | http://hdl.handle.net/10256/25116 |
| Access Level: | acceso abierto |
| Palabra clave: | Porcs -- Espermatozoides -- Investigació Swine -- Spermatozoa -- Research Crioconservació d'òrgans, teixits, etc. Cryopreservation of organs, tissues, etc. Porcs -- Recursos germoplasmàtics -- Crioconservació Swine -- Germplasm resources -- Cryopreservation |
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Insights into crucial molecules and protein channels involved in pig sperm cryopreservationDelgado Bermúdez, AriadnaPorcs -- Espermatozoides -- InvestigacióSwine -- Spermatozoa -- ResearchCrioconservació d'òrgans, teixits, etc.Cryopreservation of organs, tissues, etc.Porcs -- Recursos germoplasmàtics -- CrioconservacióSwine -- Germplasm resources -- CryopreservationCryopreservation is the most efficient procedure for long-term preservation of mammalian sperm; however, its use is not currently dominant for boar sperm before its use for artificial insemination. In fact, freezing and thawing have an extensive detrimental effect on sperm function and lead to impaired fertility. The present work summarises the basis of the structural and functional impact of cryopreservation on pig sperm that have been extensively studied in recent decades, as well as the molecular alterations in sperm that are related to this damage. The wide variety of mechanisms underlying the consequences of alterations in expression levels and structural modifications of sperm proteins with diverse functions is detailed. Moreover, the use of cryotolerance biomarkers as predictors of the potential resilience of a sperm sample to the cryopreservation process is also discussed. Regarding the proteins that have been identified to be relevant during the cryopreservation process, they are classified according to the functions they carry out in sperm, including antioxidant function, plasma membrane protection, sperm motility regulation, chromatin structure, metabolism and mitochondrial function, heat-shock response, premature capacitation and sperm-oocyte binding and fusion. Special reference is made to the relevance of sperm membrane channels, as their function is crucial for boar sperm to withstand osmotic shock during cryopreservation. Finally, potential aims for future research on cryodamage and cryotolerance are proposed, which might be crucial to minimise the side-effects of cryopreservation and to make it a more advantageous strategy for boar sperm preservationOpen Access funding was provided thanks to the CRUE-CSIC agreement with ElsevierElsevier2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/25116http://hdl.handle.net/10256/25116Animal Reproduction Science, 2024, vol. 269, art. núm. 107547Articles publicats (D-B)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2024.107547info:eu-repo/semantics/altIdentifier/issn/0378-4320info:eu-repo/semantics/altIdentifier/eissn/1873-2232Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/251162026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation |
| title |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation |
| spellingShingle |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation Delgado Bermúdez, Ariadna Porcs -- Espermatozoides -- Investigació Swine -- Spermatozoa -- Research Crioconservació d'òrgans, teixits, etc. Cryopreservation of organs, tissues, etc. Porcs -- Recursos germoplasmàtics -- Crioconservació Swine -- Germplasm resources -- Cryopreservation |
| title_short |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation |
| title_full |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation |
| title_fullStr |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation |
| title_full_unstemmed |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation |
| title_sort |
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation |
| dc.creator.none.fl_str_mv |
Delgado Bermúdez, Ariadna |
| author |
Delgado Bermúdez, Ariadna |
| author_facet |
Delgado Bermúdez, Ariadna |
| author_role |
author |
| dc.subject.none.fl_str_mv |
Porcs -- Espermatozoides -- Investigació Swine -- Spermatozoa -- Research Crioconservació d'òrgans, teixits, etc. Cryopreservation of organs, tissues, etc. Porcs -- Recursos germoplasmàtics -- Crioconservació Swine -- Germplasm resources -- Cryopreservation |
| topic |
Porcs -- Espermatozoides -- Investigació Swine -- Spermatozoa -- Research Crioconservació d'òrgans, teixits, etc. Cryopreservation of organs, tissues, etc. Porcs -- Recursos germoplasmàtics -- Crioconservació Swine -- Germplasm resources -- Cryopreservation |
| description |
Cryopreservation is the most efficient procedure for long-term preservation of mammalian sperm; however, its use is not currently dominant for boar sperm before its use for artificial insemination. In fact, freezing and thawing have an extensive detrimental effect on sperm function and lead to impaired fertility. The present work summarises the basis of the structural and functional impact of cryopreservation on pig sperm that have been extensively studied in recent decades, as well as the molecular alterations in sperm that are related to this damage. The wide variety of mechanisms underlying the consequences of alterations in expression levels and structural modifications of sperm proteins with diverse functions is detailed. Moreover, the use of cryotolerance biomarkers as predictors of the potential resilience of a sperm sample to the cryopreservation process is also discussed. Regarding the proteins that have been identified to be relevant during the cryopreservation process, they are classified according to the functions they carry out in sperm, including antioxidant function, plasma membrane protection, sperm motility regulation, chromatin structure, metabolism and mitochondrial function, heat-shock response, premature capacitation and sperm-oocyte binding and fusion. Special reference is made to the relevance of sperm membrane channels, as their function is crucial for boar sperm to withstand osmotic shock during cryopreservation. Finally, potential aims for future research on cryodamage and cryotolerance are proposed, which might be crucial to minimise the side-effects of cryopreservation and to make it a more advantageous strategy for boar sperm preservation |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10256/25116 http://hdl.handle.net/10256/25116 |
| url |
http://hdl.handle.net/10256/25116 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2024.107547 info:eu-repo/semantics/altIdentifier/issn/0378-4320 info:eu-repo/semantics/altIdentifier/eissn/1873-2232 |
| dc.rights.none.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
Animal Reproduction Science, 2024, vol. 269, art. núm. 107547 Articles publicats (D-B) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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15,81155 |