Generación de mutantes de inserción de tomate cultivado y silvestre e identificación de genes implicados en procesos de desarrollo y tolerancia a estrés abiótico
[EN] When addressing the genetic dissection of a complex trait, what really matters is the identification of the genes with major effects, because their modification may result in qualitative changes in the phenotype. For this purpose, a mutagenesis-based approach has two advantages: first, the iden...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/63148 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/63148 |
| Access Level: | acceso abierto |
| Palabra clave: | Tomate Solanum pimpinellifolium Solanum cheesmaniae Mutagénesis insercional con T-DNA Genes desarrollo Genes estrés abiótico Tolerancia salinidad GENETICA |
| Sumario: | [EN] When addressing the genetic dissection of a complex trait, what really matters is the identification of the genes with major effects, because their modification may result in qualitative changes in the phenotype. For this purpose, a mutagenesis-based approach has two advantages: first, the identification of a mutant reveals that the altered gene has a key effect on the trait; secondly, the phenotypic characterization of the mutant allows making an inference about the gene function. Insertion mutagenesis by T-DNA provides an additional advantage: a gene tagged by a T-DNA insert can be easily identified using PCR-based techniques (e.g. Anchor- PCR). In order to identify genes that control developmental traits and abiotic stress tolerance in tomato and wild related species, we are performing a program of insertion mutagenesis in collaboration with the groups of Dr. Rafael Lozano (University of Almeria) and Dr. MªCarmen Bolarín (CEBAS-Murcia). The objectives of the present Doctoral Thesis are framed in the context of this insertion mutagenesis program in tomato and wild relatives. First, in order to expand the collections of T-DNA lines previously generated in our group, 952 T-DNA lines of tomato, 405 of Solanum pimpinellifolium and 550 of S. cheesmaniae have been obtained. Secondly we performed the evaluation of progenies from 1545 T-DNA lines of tomato, 194 T-DNA lines of S. pimpinellifolium and 149 T-DNA lines of S. cheesmaniae. The screening in vitro of those progenies allowed us to identify 43 mutants altered in early developmental traits. In addition, we were able to detect three mutants of tomato and another one of S. cheesmaniae which are hypersensitive to salt stress. The phenotypic and genetic characterization of selected mutants has been carried out. Finally, we performed the functional analysis of the PMS (PROTECTING MERISTEMS AGAINST SALINITY) gene tagged in the pms-916 tomato mutant. Our results suggest that the PMS gene plays an essential role in the protection of the shoot apical meristem and young tissues of the tomato plant under salinity stress conditions. |
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