Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities

Open Access funding enabled and organized by CRUE/BUCLE 2025 Gold

Detalles Bibliográficos
Autores: Nistal García, Alejandro, García García, Pedro, Albero Martínez, Luis, Bécares Mantecón, Eloy, García Girón, Jorge
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/27535
Acceso en línea:https://hdl.handle.net/10612/27535
Access Level:acceso abierto
Palabra clave:Ecología. Medio ambiente
Amphibians
DNA metabarcoding
2401.06 Ecología Animal
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spelling Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian CommunitiesNistal García, AlejandroGarcía García, PedroAlbero Martínez, LuisBécares Mantecón, EloyGarcía Girón, JorgeEcología. Medio ambienteAmphibiansDNA metabarcoding2401.06 Ecología AnimalOpen Access funding enabled and organized by CRUE/BUCLE 2025 Gold[EN] Amphibians are facing large population declines as a result of both natural and anthropogenic threats. Reconstructing andunderstanding the composition and structure of amphibian communities is essential for developing effective monitoring andconservation strategies. In this study, we compared environmental DNA metabarcoding (eDNA-MB) data with a comprehen-sive dataset obtained from acoustic and visual encounter surveys (AVES) in a set of Mediterranean ponds. Additionally, wedesigned and validated a quantitative PCR (qPCR) assay for the Iberian ribbed newt (Pleurodeles waltl) and tested its abilitywith eDNA-MB and AVES in assessing the presence and population densities of this species. Our findings provide evidence thateDNA-MB can be a valuable method for reconstructing the composition and structure of amphibian communities. eDNA-MBidentified all expected amphibian species from the regional species pool, including some that were missed in traditional surveys.Although eDNA-MB abundance estimations for P. waltl seemed virtually independent of population densities obtained throughfield surveys, we found a significant association between eDNA concentration (qPCR) and AVES abundance estimates. Ourfindings suggest that effective strategies for amphibian biomonitoring need the combination of community data obtained fromboth traditional and eDNA surveys, especially in the Mediterranean region where strong environmental constraints can affectthe detection and persistence of eDNA in freshwater ecosystems.SIWileyEcologiaFacultad de Ciencias Biologicas y Ambientales2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/10612/27535reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/AEI/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia /PID2022-140081OB-I00http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/275352026-06-24T12:43:27Z
dc.title.none.fl_str_mv Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
title Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
spellingShingle Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
Nistal García, Alejandro
Ecología. Medio ambiente
Amphibians
DNA metabarcoding
2401.06 Ecología Animal
title_short Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
title_full Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
title_fullStr Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
title_full_unstemmed Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
title_sort Environmental DNA (eDNA) and Field Surveys as Complementary Tools for the Biomonitoring of Amphibian Communities
dc.creator.none.fl_str_mv Nistal García, Alejandro
García García, Pedro
Albero Martínez, Luis
Bécares Mantecón, Eloy
García Girón, Jorge
author Nistal García, Alejandro
author_facet Nistal García, Alejandro
García García, Pedro
Albero Martínez, Luis
Bécares Mantecón, Eloy
García Girón, Jorge
author_role author
author2 García García, Pedro
Albero Martínez, Luis
Bécares Mantecón, Eloy
García Girón, Jorge
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ecologia
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Ecología. Medio ambiente
Amphibians
DNA metabarcoding
2401.06 Ecología Animal
topic Ecología. Medio ambiente
Amphibians
DNA metabarcoding
2401.06 Ecología Animal
description Open Access funding enabled and organized by CRUE/BUCLE 2025 Gold
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10612/27535
url https://hdl.handle.net/10612/27535
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia /PID2022-140081OB-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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