Applications of mass spectrometry for the determination of the microbial crude protein synthesis in ruminants
The importance of quantifying ruminal microbial crude protein synthesis has promoted the development and comparison of several different methods for precise determination of both the amount and rate of synthesis. One major challenge is in estimating and differentiating protein in the rumen between m...
| Autores: | , , , |
|---|---|
| Tipo de recurso: | capítulo de libro |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/70755 |
| Acceso en línea: | https://doi.org/10.2174/97898114338251200101 http://hdl.handle.net/10459.1/70755 |
| Access Level: | acceso abierto |
| Palabra clave: | Microbial protein Endogenous excretion Ruminants Purine derivatives |
| Sumario: | The importance of quantifying ruminal microbial crude protein synthesis has promoted the development and comparison of several different methods for precise determination of both the amount and rate of synthesis. One major challenge is in estimating and differentiating protein in the rumen between microbial, dietary, and endogenous fractions, and to correctly isolate the solid and liquid microbial fraction of the rumen contents. This is further complicated by the goal of using non-invasive methods as much as is feasible, such as avoiding the use of fistulated animals; the selection of an appropriate microbial marker, specifically one that behaves similarly in the solid-associated and liquid-associated microbial fractions. It is also vital to be able to accurately estimate the contribution of microbial protein to overall nitrogen used by the animal, which can be accomplished by the use of 15N labeled, as assimilated by ruminal bacteria, and by the quantification of labeled nitrogen via mass spectrometry (15N/14N). This review focuses on challenges regarding accurate quantification of microbial crude protein synthesis in the rumen, as well as providing the methodology for quantification using the 15N marker. This review is based on the collection of scientific papers from the main research groups in feed and animal nutrition in ruminants. |
|---|