Gαq activation modulates autophagy by promoting mTORC1 signaling.

The mTORC1 node plays a major role in autophagy modulation. We report a role of the ubiquitous Gαq subunit, a known transducer of plasma membrane G protein-coupled receptors signaling, as a core modulator of mTORC1 and autophagy. Cells lacking Gαq/11 display higher basal autophagy, enhanced autophag...

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Detalles Bibliográficos
Autores: Cabezudo, Sofía, Sanz-Flores, Maria, Caballero, Alvaro, Tasset, Inmaculada, Rebollo, Elena, Diaz, Antonio, Aragay, Anna M, Cuervo, Ana María, Mayor, Federico, Ribas, Catalina
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/13322
Acceso en línea:http://hdl.handle.net/20.500.12105/13322
Access Level:acceso abierto
Palabra clave:Autophagy
Signal Transduction
Animals
CHO Cells
Cricetulus
Fibroblasts
GTP-Binding Protein alpha Subunits, Gq-G11
HEK293 Cells
Humans
Lysosomes
Male
Mechanistic Target of Rapamycin Complex 1
Mice
Models, Biological
Phenotype
Protein Binding
Protein Domains
Rats, Wistar
Regulatory-Associated Protein of mTOR
Sequestosome-1 Protein
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oai_identifier_str oai:repisalud.isciii.es:20.500.12105/13322
network_acronym_str ES
network_name_str España
repository_id_str
spelling Gαq activation modulates autophagy by promoting mTORC1 signaling.Cabezudo, SofíaSanz-Flores, MariaCaballero, AlvaroTasset, InmaculadaRebollo, ElenaDiaz, AntonioAragay, Anna MCuervo, Ana MaríaMayor, FedericoRibas, CatalinaAutophagySignal TransductionAnimalsCHO CellsCricetulusFibroblastsGTP-Binding Protein alpha Subunits, Gq-G11HEK293 CellsHumansLysosomesMaleMechanistic Target of Rapamycin Complex 1MiceModels, BiologicalPhenotypeProtein BindingProtein DomainsRats, WistarRegulatory-Associated Protein of mTORSequestosome-1 ProteinThe mTORC1 node plays a major role in autophagy modulation. We report a role of the ubiquitous Gαq subunit, a known transducer of plasma membrane G protein-coupled receptors signaling, as a core modulator of mTORC1 and autophagy. Cells lacking Gαq/11 display higher basal autophagy, enhanced autophagy induction upon different types of nutrient stress along with a decreased mTORC1 activation status. They are also unable to reactivate mTORC1 and thus inactivate ongoing autophagy upon nutrient recovery. Conversely, stimulation of Gαq/11 promotes sustained mTORC1 pathway activation and reversion of autophagy promoted by serum or amino acids removal. Gαq is present in autophagic compartments and lysosomes and is part of the mTORC1 multi-molecular complex, contributing to its assembly and activation via its nutrient status-sensitive interaction with p62, which displays features of a Gαq effector. Gαq emerges as a central regulator of the autophagy machinery required to maintain cellular homeostasis upon nutrient fluctuations.Nature Publishing GroupMinisterio de Economía, Industria y Competitividad (España)Instituto de Salud Carlos IIIUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)20212021-08-2720212021-07-2720212021-07-27journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/20.500.12105/13322reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/133222026-06-12T12:43:37Z
dc.title.none.fl_str_mv Gαq activation modulates autophagy by promoting mTORC1 signaling.
title Gαq activation modulates autophagy by promoting mTORC1 signaling.
spellingShingle Gαq activation modulates autophagy by promoting mTORC1 signaling.
Cabezudo, Sofía
Autophagy
Signal Transduction
Animals
CHO Cells
Cricetulus
Fibroblasts
GTP-Binding Protein alpha Subunits, Gq-G11
HEK293 Cells
Humans
Lysosomes
Male
Mechanistic Target of Rapamycin Complex 1
Mice
Models, Biological
Phenotype
Protein Binding
Protein Domains
Rats, Wistar
Regulatory-Associated Protein of mTOR
Sequestosome-1 Protein
title_short Gαq activation modulates autophagy by promoting mTORC1 signaling.
title_full Gαq activation modulates autophagy by promoting mTORC1 signaling.
title_fullStr Gαq activation modulates autophagy by promoting mTORC1 signaling.
title_full_unstemmed Gαq activation modulates autophagy by promoting mTORC1 signaling.
title_sort Gαq activation modulates autophagy by promoting mTORC1 signaling.
dc.creator.none.fl_str_mv Cabezudo, Sofía
Sanz-Flores, Maria
Caballero, Alvaro
Tasset, Inmaculada
Rebollo, Elena
Diaz, Antonio
Aragay, Anna M
Cuervo, Ana María
Mayor, Federico
Ribas, Catalina
author Cabezudo, Sofía
author_facet Cabezudo, Sofía
Sanz-Flores, Maria
Caballero, Alvaro
Tasset, Inmaculada
Rebollo, Elena
Diaz, Antonio
Aragay, Anna M
Cuervo, Ana María
Mayor, Federico
Ribas, Catalina
author_role author
author2 Sanz-Flores, Maria
Caballero, Alvaro
Tasset, Inmaculada
Rebollo, Elena
Diaz, Antonio
Aragay, Anna M
Cuervo, Ana María
Mayor, Federico
Ribas, Catalina
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
Instituto de Salud Carlos III
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.subject.none.fl_str_mv Autophagy
Signal Transduction
Animals
CHO Cells
Cricetulus
Fibroblasts
GTP-Binding Protein alpha Subunits, Gq-G11
HEK293 Cells
Humans
Lysosomes
Male
Mechanistic Target of Rapamycin Complex 1
Mice
Models, Biological
Phenotype
Protein Binding
Protein Domains
Rats, Wistar
Regulatory-Associated Protein of mTOR
Sequestosome-1 Protein
topic Autophagy
Signal Transduction
Animals
CHO Cells
Cricetulus
Fibroblasts
GTP-Binding Protein alpha Subunits, Gq-G11
HEK293 Cells
Humans
Lysosomes
Male
Mechanistic Target of Rapamycin Complex 1
Mice
Models, Biological
Phenotype
Protein Binding
Protein Domains
Rats, Wistar
Regulatory-Associated Protein of mTOR
Sequestosome-1 Protein
description The mTORC1 node plays a major role in autophagy modulation. We report a role of the ubiquitous Gαq subunit, a known transducer of plasma membrane G protein-coupled receptors signaling, as a core modulator of mTORC1 and autophagy. Cells lacking Gαq/11 display higher basal autophagy, enhanced autophagy induction upon different types of nutrient stress along with a decreased mTORC1 activation status. They are also unable to reactivate mTORC1 and thus inactivate ongoing autophagy upon nutrient recovery. Conversely, stimulation of Gαq/11 promotes sustained mTORC1 pathway activation and reversion of autophagy promoted by serum or amino acids removal. Gαq is present in autophagic compartments and lysosomes and is part of the mTORC1 multi-molecular complex, contributing to its assembly and activation via its nutrient status-sensitive interaction with p62, which displays features of a Gαq effector. Gαq emerges as a central regulator of the autophagy machinery required to maintain cellular homeostasis upon nutrient fluctuations.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-08-27
2021
2021-07-27
2021
2021-07-27
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/13322
url http://hdl.handle.net/20.500.12105/13322
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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