Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe

Background Calcium/calmodulin-dependent protein kinase kinase (CaMKK) is required for diverse cellular functions. Mammalian CaMKK activates CaMKs and also the evolutionarily-conserved AMP-activated protein kinase (AMPK). The fission yeast Schizosaccharomyces pombe CaMKK, Ssp1, is required for tolera...

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Autores: Gómez Hierro, Alba, Lambea Martínez, Eva, Giménez Zaragoza, David, López Avilés, Sandra, Yance Chávez, Tula del Carmen, Montserrat, Marta, Pujol Sobrevía, María Jesús, Bachs Valldeneu, Oriol, Aligué i Alemany, Rosa Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/102334
Acceso en línea:https://hdl.handle.net/2445/102334
Access Level:acceso abierto
Palabra clave:Cicle cel·lular
Proteïnes quinases
Mitosi
Cell cycle
Protein kinases
Mitosis
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spelling Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombeGómez Hierro, AlbaLambea Martínez, EvaGiménez Zaragoza, DavidLópez Avilés, SandraYance Chávez, Tula del CarmenMontserrat, MartaPujol Sobrevía, María JesúsBachs Valldeneu, OriolAligué i Alemany, Rosa MariaCicle cel·lularProteïnes quinasesMitosiCell cycleProtein kinasesMitosisBackground Calcium/calmodulin-dependent protein kinase kinase (CaMKK) is required for diverse cellular functions. Mammalian CaMKK activates CaMKs and also the evolutionarily-conserved AMP-activated protein kinase (AMPK). The fission yeast Schizosaccharomyces pombe CaMKK, Ssp1, is required for tolerance to limited glucose through the AMPK, Ssp2, and for the integration of cell growth and division through the SAD kinase Cdr2. Results Here we report that Ssp1 controls the G2/M transition by regulating the activity of the CaMK Srk1. We show that inhibition of Cdc25 by Srk1 is regulated by Ssp1; and also that restoring growth polarity and actin localization of ssp1-deleted cells by removing the actin-monomer-binding protein, twinfilin, is sufficient to suppress the ssp1 phenotype. Conclusions These findings demonstrate that entry into mitosis is mediated by a network of proteins, including the Ssp1 and Srk1 kinases. Ssp1 connects the network of components that ensures proper polarity and cell size with the network of proteins that regulates Cdk1-cyclin B activity, in which Srk1 plays an inhibitory role.Public Library of Science (PLoS)2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/102334Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0143037PLoS One, 2015, vol. 10, num. 11, p. e0143037http://dx.doi.org/10.1371/journal.pone.0143037cc-by (c) Gómez Hierro, Alba et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1023342026-05-27T06:46:51Z
dc.title.none.fl_str_mv Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
title Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
spellingShingle Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
Gómez Hierro, Alba
Cicle cel·lular
Proteïnes quinases
Mitosi
Cell cycle
Protein kinases
Mitosis
title_short Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
title_full Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
title_fullStr Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
title_full_unstemmed Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
title_sort Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe
dc.creator.none.fl_str_mv Gómez Hierro, Alba
Lambea Martínez, Eva
Giménez Zaragoza, David
López Avilés, Sandra
Yance Chávez, Tula del Carmen
Montserrat, Marta
Pujol Sobrevía, María Jesús
Bachs Valldeneu, Oriol
Aligué i Alemany, Rosa Maria
author Gómez Hierro, Alba
author_facet Gómez Hierro, Alba
Lambea Martínez, Eva
Giménez Zaragoza, David
López Avilés, Sandra
Yance Chávez, Tula del Carmen
Montserrat, Marta
Pujol Sobrevía, María Jesús
Bachs Valldeneu, Oriol
Aligué i Alemany, Rosa Maria
author_role author
author2 Lambea Martínez, Eva
Giménez Zaragoza, David
López Avilés, Sandra
Yance Chávez, Tula del Carmen
Montserrat, Marta
Pujol Sobrevía, María Jesús
Bachs Valldeneu, Oriol
Aligué i Alemany, Rosa Maria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cicle cel·lular
Proteïnes quinases
Mitosi
Cell cycle
Protein kinases
Mitosis
topic Cicle cel·lular
Proteïnes quinases
Mitosi
Cell cycle
Protein kinases
Mitosis
description Background Calcium/calmodulin-dependent protein kinase kinase (CaMKK) is required for diverse cellular functions. Mammalian CaMKK activates CaMKs and also the evolutionarily-conserved AMP-activated protein kinase (AMPK). The fission yeast Schizosaccharomyces pombe CaMKK, Ssp1, is required for tolerance to limited glucose through the AMPK, Ssp2, and for the integration of cell growth and division through the SAD kinase Cdr2. Results Here we report that Ssp1 controls the G2/M transition by regulating the activity of the CaMK Srk1. We show that inhibition of Cdc25 by Srk1 is regulated by Ssp1; and also that restoring growth polarity and actin localization of ssp1-deleted cells by removing the actin-monomer-binding protein, twinfilin, is sufficient to suppress the ssp1 phenotype. Conclusions These findings demonstrate that entry into mitosis is mediated by a network of proteins, including the Ssp1 and Srk1 kinases. Ssp1 connects the network of components that ensures proper polarity and cell size with the network of proteins that regulates Cdk1-cyclin B activity, in which Srk1 plays an inhibitory role.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/102334
url https://hdl.handle.net/2445/102334
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0143037
PLoS One, 2015, vol. 10, num. 11, p. e0143037
http://dx.doi.org/10.1371/journal.pone.0143037
dc.rights.none.fl_str_mv cc-by (c) Gómez Hierro, Alba et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Gómez Hierro, Alba et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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