Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles

Ubiquitination is a relevant cell regulatory mechanism to determine protein fate and function. Most data has focused on the role of ubiquitin as a tag molecule to target substrates to proteasome degradation, and on its impact in the control of cell cycle, protein homeostasis and cancer. Only recentl...

ver descrição completa

Detalhes bibliográficos
Autores: Esquerdo Barragán, Mariona, Grau Bové, Francesc Xavier, Garanto, Alejandro, Toulis, Vasileios, Garcia Monclús, Silvia, Millo, Erica, López-Iniesta, Mª José, Abad-Morales, Víctor, Ruiz Trillo, Iñaki, Marfany i Nadal, Gemma
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/96202
Acesso em linha:https://hdl.handle.net/2445/96202
Access Level:acceso abierto
Palavra-chave:Ubiqüitina
Vertebrats
Retina
Fenotip
Ubiquitin
Vertebrates
Phenotype
id ES_beda2c40446e60c8f6735e0727f54e06
oai_identifier_str oai:recercat.cat:2445/96202
network_acronym_str ES
network_name_str España
repository_id_str
spelling Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic rolesEsquerdo Barragán, MarionaGrau Bové, Francesc XavierGaranto, AlejandroToulis, VasileiosGarcia Monclús, SilviaMillo, EricaLópez-Iniesta, Mª JoséAbad-Morales, VíctorRuiz Trillo, IñakiMarfany i Nadal, GemmaUbiqüitinaVertebratsRetinaFenotipUbiquitinVertebratesRetinaPhenotypeUbiquitination is a relevant cell regulatory mechanism to determine protein fate and function. Most data has focused on the role of ubiquitin as a tag molecule to target substrates to proteasome degradation, and on its impact in the control of cell cycle, protein homeostasis and cancer. Only recently, systematic assays have pointed to the relevance of the ubiquitin pathway in the development and differentiation of tissues and organs, and its implication in hereditary diseases. Moreover, although the activity and composition of ubiquitin ligases has been largely addressed, the role of the deubiquitinating enzymes (DUBs) in specific tissues, such as the retina, remains mainly unknown. In this work, we undertook a systematic analysis of the transcriptional levels of DUB genes in the adult mouse retina by RT-qPCR and analyzed the expression pattern by in situ hybridization and fluorescent immunohistochemistry, thus providing a unique spatial reference map of retinal DUB expression. We also performed a systematic phylogenetic analysis to understand the origin and the presence/absence of DUB genes in the genomes of diverse animal taxa that represent most of the known animal diversity. The expression landscape obtained supports the potential subfunctionalization of paralogs in those families that expanded in vertebrates. Overall, our results constitute a reference framework for further characterization of the DUB roles in the retina and suggest new candidates for inherited retinal disorders.Public Library of Science (PLoS)2016201620162016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 p.application/pdfhttps://hdl.handle.net/2445/96202Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0150364PLoS One, 2016, vol. 11, num. 3, p. e0150364-e0150364http://dx.doi.org/10.1371/journal.pone.0150364cc-by (c) Esquerdo, Mariona et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/962022026-05-29T05:05:01Z
dc.title.none.fl_str_mv Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
title Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
spellingShingle Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
Esquerdo Barragán, Mariona
Ubiqüitina
Vertebrats
Retina
Fenotip
Ubiquitin
Vertebrates
Retina
Phenotype
title_short Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
title_full Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
title_fullStr Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
title_full_unstemmed Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
title_sort Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles
dc.creator.none.fl_str_mv Esquerdo Barragán, Mariona
Grau Bové, Francesc Xavier
Garanto, Alejandro
Toulis, Vasileios
Garcia Monclús, Silvia
Millo, Erica
López-Iniesta, Mª José
Abad-Morales, Víctor
Ruiz Trillo, Iñaki
Marfany i Nadal, Gemma
author Esquerdo Barragán, Mariona
author_facet Esquerdo Barragán, Mariona
Grau Bové, Francesc Xavier
Garanto, Alejandro
Toulis, Vasileios
Garcia Monclús, Silvia
Millo, Erica
López-Iniesta, Mª José
Abad-Morales, Víctor
Ruiz Trillo, Iñaki
Marfany i Nadal, Gemma
author_role author
author2 Grau Bové, Francesc Xavier
Garanto, Alejandro
Toulis, Vasileios
Garcia Monclús, Silvia
Millo, Erica
López-Iniesta, Mª José
Abad-Morales, Víctor
Ruiz Trillo, Iñaki
Marfany i Nadal, Gemma
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ubiqüitina
Vertebrats
Retina
Fenotip
Ubiquitin
Vertebrates
Retina
Phenotype
topic Ubiqüitina
Vertebrats
Retina
Fenotip
Ubiquitin
Vertebrates
Retina
Phenotype
description Ubiquitination is a relevant cell regulatory mechanism to determine protein fate and function. Most data has focused on the role of ubiquitin as a tag molecule to target substrates to proteasome degradation, and on its impact in the control of cell cycle, protein homeostasis and cancer. Only recently, systematic assays have pointed to the relevance of the ubiquitin pathway in the development and differentiation of tissues and organs, and its implication in hereditary diseases. Moreover, although the activity and composition of ubiquitin ligases has been largely addressed, the role of the deubiquitinating enzymes (DUBs) in specific tissues, such as the retina, remains mainly unknown. In this work, we undertook a systematic analysis of the transcriptional levels of DUB genes in the adult mouse retina by RT-qPCR and analyzed the expression pattern by in situ hybridization and fluorescent immunohistochemistry, thus providing a unique spatial reference map of retinal DUB expression. We also performed a systematic phylogenetic analysis to understand the origin and the presence/absence of DUB genes in the genomes of diverse animal taxa that represent most of the known animal diversity. The expression landscape obtained supports the potential subfunctionalization of paralogs in those families that expanded in vertebrates. Overall, our results constitute a reference framework for further characterization of the DUB roles in the retina and suggest new candidates for inherited retinal disorders.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/96202
url https://hdl.handle.net/2445/96202
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0150364
PLoS One, 2016, vol. 11, num. 3, p. e0150364-e0150364
http://dx.doi.org/10.1371/journal.pone.0150364
dc.rights.none.fl_str_mv cc-by (c) Esquerdo, Mariona et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Esquerdo, Mariona et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418319720742912
score 15,812429

Registos relacionados