Tetracycline and multidrug resistance in the oral microbiota

Introduction : Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis...

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Autores: Arredondo, Alexandre|||0000-0003-4459-1300, Blanc Pociello, Vanessa|||0000-0002-2740-6559, Mor, Carolina, Nart, José, León Berríos, Rubén|||0000-0001-6400-4906
Tipo de documento: artigo
Data de publicação:2020
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:252277
Acesso em linha:https://ddd.uab.cat/record/252277
https://dx.doi.org/urn:doi:10.1080/20002297.2020.1847431
Access Level:Acceso aberto
Palavra-chave:Antibiotic resistance
Tetracycline
Periodontitis
Subgingival microbiota
Multidrug resistance
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spelling Tetracycline and multidrug resistance in the oral microbiotadifferences between healthy subjects and patients with periodontitis in SpainArredondo, Alexandre|||0000-0003-4459-1300Blanc Pociello, Vanessa|||0000-0002-2740-6559Mor, CarolinaNart, JoséLeón Berríos, Rubén|||0000-0001-6400-4906Antibiotic resistanceTetracyclinePeriodontitisSubgingival microbiotaMultidrug resistanceIntroduction : Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods : Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively. Results : tet (M) was the most frequently detected tet gene (52.03%). We observed significant differences between the prevalence of tet (M), tet (W), tet (O), tet (32) and tet (L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83.64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups. Conclusion : The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/252277https://dx.doi.org/urn:doi:10.1080/20002297.2020.1847431reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2522772026-06-06T12:50:31Z
dc.title.none.fl_str_mv Tetracycline and multidrug resistance in the oral microbiota
differences between healthy subjects and patients with periodontitis in Spain
title Tetracycline and multidrug resistance in the oral microbiota
spellingShingle Tetracycline and multidrug resistance in the oral microbiota
Arredondo, Alexandre|||0000-0003-4459-1300
Antibiotic resistance
Tetracycline
Periodontitis
Subgingival microbiota
Multidrug resistance
title_short Tetracycline and multidrug resistance in the oral microbiota
title_full Tetracycline and multidrug resistance in the oral microbiota
title_fullStr Tetracycline and multidrug resistance in the oral microbiota
title_full_unstemmed Tetracycline and multidrug resistance in the oral microbiota
title_sort Tetracycline and multidrug resistance in the oral microbiota
dc.creator.none.fl_str_mv Arredondo, Alexandre|||0000-0003-4459-1300
Blanc Pociello, Vanessa|||0000-0002-2740-6559
Mor, Carolina
Nart, José
León Berríos, Rubén|||0000-0001-6400-4906
author Arredondo, Alexandre|||0000-0003-4459-1300
author_facet Arredondo, Alexandre|||0000-0003-4459-1300
Blanc Pociello, Vanessa|||0000-0002-2740-6559
Mor, Carolina
Nart, José
León Berríos, Rubén|||0000-0001-6400-4906
author_role author
author2 Blanc Pociello, Vanessa|||0000-0002-2740-6559
Mor, Carolina
Nart, José
León Berríos, Rubén|||0000-0001-6400-4906
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antibiotic resistance
Tetracycline
Periodontitis
Subgingival microbiota
Multidrug resistance
topic Antibiotic resistance
Tetracycline
Periodontitis
Subgingival microbiota
Multidrug resistance
description Introduction : Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods : Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively. Results : tet (M) was the most frequently detected tet gene (52.03%). We observed significant differences between the prevalence of tet (M), tet (W), tet (O), tet (32) and tet (L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83.64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups. Conclusion : The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
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https://dx.doi.org/urn:doi:10.1080/20002297.2020.1847431
url https://ddd.uab.cat/record/252277
https://dx.doi.org/urn:doi:10.1080/20002297.2020.1847431
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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