One-step nucleic acid amplification (Osna) of sentinel lymph node in early-stage endometrial cancer: Spanish multicenter study (endo-osna)

The objective of this study was to evaluate the efficacy of one-step nucleic acid amplification (OSNA) for the detection of sentinel lymph node (SLN) metastasis compared to standard pathological ultrastaging in patients with early-stage endometrial cancer (EC). A total of 526 SLNs from 191 patients...

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Detalles Bibliográficos
Autores: Diestro Tejada, María Dolores, Sánchez-Pastor Ruiz, Margarita, López Cuadrado, María Teresa, Berjón, Alberto, Zapardiel Gutiérrez, Ignacio, Yébenes, Laura, Ruiz, Irune, Lekuona, Arantza, Rezola, Marta, Jaunarena, Ibon, Siegrist, Jaime, Sagasta, Amaia, Guerra, Isabel, Lete, Luis I., Roldán, Fernando, Marta, Carlo B., Boillos, María J., Cardiel, María J., Carlos, López de la Manzanara, Relea, Fernanda, Coronado, Pluvio J., Pascual, Alejandro, Román, María J., Peiró, Gloria, Matute, Luis J., Montero, Beatriz, Muruzábal, Juan C., Guarch, Rosa, Zorrero, Cristina, Calatrava, Ana, Ribot, Laia, Costa, Irmgard, Hernández, Alicia, Hardisson Hernáez, David Alonso
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/716676
Acceso en línea:http://hdl.handle.net/10486/716676
https://dx.doi.org/10.3390/cancers13174465
Access Level:acceso abierto
Palabra clave:Cytokeratin 19
Endometrial cancer
Micrometastases
One-step nucleic acid amplification
OSNA
Sentinel lymph node
Ultrastaging
Medicina
Descripción
Sumario:The objective of this study was to evaluate the efficacy of one-step nucleic acid amplification (OSNA) for the detection of sentinel lymph node (SLN) metastasis compared to standard pathological ultrastaging in patients with early-stage endometrial cancer (EC). A total of 526 SLNs from 191 patients with EC were included in the study, and 379 SLNs (147 patients) were evaluated by both methods, OSNA and standard pathological ultrastaging. The central 1 mm portion of each lymph node was subjected to semi-serial sectioning at 200 µm intervals and examined by hematoxylin–eosin and immunohistochemistry with CK19; the remaining tissue was analyzed by OSNA for CK19 mRNA. The OSNA assay detected metastases in 19.7% of patients (14.9% micrometastasis and 4.8% macrometastasis), whereas pathological ultrastaging detected metastasis in 8.8% of patients (3.4% micrometastasis and 5.4% macrometastasis). Using the established cut-off value for detecting SLN metastasis by OSNA in EC (250 copies/µL), the sensitivity of the OSNA assay was 92%, specificity was 82%, diagnostic accuracy was 83%, and the negative predictive value was 99%. Discordant results between both methods were recorded in 20 patients (13.6%). OSNA resulted in an upstaging in 12 patients (8.2%). OSNA could aid in the identification of patients requiring adjuvant treatment at the time of diagnosis

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