Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)

17 Pág. Departamento de Reproducción animal

Detalles Bibliográficos
Autores: Fuentes-Albero, María Del Carmen, González-Brusi, Leopoldo, Cots, Paula, Luongo, Chiara, Abril-Sánchez, S., Ros-Santaella, José Luis, Pintus, Eliana, Ruiz-Díaz, Sara, Barros-García, Carlos, Sánchez-Calabuig, M. J., García-Parraga, Daniel, Avilés, Manuel, Izquierdo-Rico, Mª José, García-Vázquez, Francisco Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/286791
Acceso en línea:http://hdl.handle.net/10261/286791
https://api.elsevier.com/content/abstract/scopus_id/85111627268
Access Level:acceso abierto
Palabra clave:Cetacean
Proteomic analyses
Semen
Seminal plasma
Sperm cells
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repository_id_str
spelling Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)Fuentes-Albero, María Del CarmenGonzález-Brusi, LeopoldoCots, PaulaLuongo, ChiaraAbril-Sánchez, S.Ros-Santaella, José LuisPintus, ElianaRuiz-Díaz, SaraBarros-García, CarlosSánchez-Calabuig, M. J.García-Parraga, DanielAvilés, ManuelIzquierdo-Rico, Mª JoséGarcía-Vázquez, Francisco AlbertoCetaceanProteomic analysesSemenSeminal plasmaSperm cells17 Pág. Departamento de Reproducción animalProteins play an important role in many reproductive functions such as sperm maturation, sperm transit in the female genital tract or sperm-oocyte interaction. However, in general, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using an LC-MS/MS, and a list with the gene symbols corresponding to each protein was submitted to the DAVID database. Of the 419 proteins identified in spermatozoa and 303 in seminal plasma, 111 proteins were shared by both. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The five most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GSTM3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this study provides the first characterization of the proteome in cetacean sperm and seminal plasma, opening the way to future research into new biomarkers, the analysis of conservation capacity or possible additional applications in the field of assisted reproductive technologies.This research was supported by the Spanish Ministry of Science and Innovation (PID2019-106380RB-I00/AEI/10.1303/501100011033) and PGC2018-094781-B-I00 (MCINN/AEI/FEDER, UE), and Oceanográfica Foundation.Peer reviewedFrontiers MediaMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionFundación OceanogràficFuentes-Albero, María Del Carmen [0000-0002-2612-674X]González-Brusi, Leopoldo [0000-0002-6371-6423]Cots, Paula [0000-0001-7630-1043)]Luongo, Chiara [0000-0003-0812-7033]Abril-Sánchez, S. [0000-0003-0963-6809]Ros-Santaella, José Luis [0000-0002-4186-7730]Pintus, Eliana [0000-0002-8496-0820]Ruiz-Díaz, Sara [0000-0002-3864-0156]Sánchez-Calabuig, M. J. [0000-0002-4280-992X]García-Parraga, Daniel [0000-0002-3335-5831]Avilés, Manuel [0000-0003-4396-4718]Izquierdo-Rico, Mª José [0000-0001-9438-6933]García-Vázquez, Francisco Alberto [0000-0001-7665-3858]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/286791https://api.elsevier.com/content/abstract/scopus_id/85111627268reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106380RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-094781-B-I00Frontiers in cell and developmental biologyhttps://doi.org/10.3389/fcell.2021.673961Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2867912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
title Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
spellingShingle Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
Fuentes-Albero, María Del Carmen
Cetacean
Proteomic analyses
Semen
Seminal plasma
Sperm cells
title_short Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
title_full Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
title_fullStr Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
title_full_unstemmed Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
title_sort Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
dc.creator.none.fl_str_mv Fuentes-Albero, María Del Carmen
González-Brusi, Leopoldo
Cots, Paula
Luongo, Chiara
Abril-Sánchez, S.
Ros-Santaella, José Luis
Pintus, Eliana
Ruiz-Díaz, Sara
Barros-García, Carlos
Sánchez-Calabuig, M. J.
García-Parraga, Daniel
Avilés, Manuel
Izquierdo-Rico, Mª José
García-Vázquez, Francisco Alberto
author Fuentes-Albero, María Del Carmen
author_facet Fuentes-Albero, María Del Carmen
González-Brusi, Leopoldo
Cots, Paula
Luongo, Chiara
Abril-Sánchez, S.
Ros-Santaella, José Luis
Pintus, Eliana
Ruiz-Díaz, Sara
Barros-García, Carlos
Sánchez-Calabuig, M. J.
García-Parraga, Daniel
Avilés, Manuel
Izquierdo-Rico, Mª José
García-Vázquez, Francisco Alberto
author_role author
author2 González-Brusi, Leopoldo
Cots, Paula
Luongo, Chiara
Abril-Sánchez, S.
Ros-Santaella, José Luis
Pintus, Eliana
Ruiz-Díaz, Sara
Barros-García, Carlos
Sánchez-Calabuig, M. J.
García-Parraga, Daniel
Avilés, Manuel
Izquierdo-Rico, Mª José
García-Vázquez, Francisco Alberto
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Fundación Oceanogràfic
Fuentes-Albero, María Del Carmen [0000-0002-2612-674X]
González-Brusi, Leopoldo [0000-0002-6371-6423]
Cots, Paula [0000-0001-7630-1043)]
Luongo, Chiara [0000-0003-0812-7033]
Abril-Sánchez, S. [0000-0003-0963-6809]
Ros-Santaella, José Luis [0000-0002-4186-7730]
Pintus, Eliana [0000-0002-8496-0820]
Ruiz-Díaz, Sara [0000-0002-3864-0156]
Sánchez-Calabuig, M. J. [0000-0002-4280-992X]
García-Parraga, Daniel [0000-0002-3335-5831]
Avilés, Manuel [0000-0003-4396-4718]
Izquierdo-Rico, Mª José [0000-0001-9438-6933]
García-Vázquez, Francisco Alberto [0000-0001-7665-3858]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cetacean
Proteomic analyses
Semen
Seminal plasma
Sperm cells
topic Cetacean
Proteomic analyses
Semen
Seminal plasma
Sperm cells
description 17 Pág. Departamento de Reproducción animal
publishDate 2021
dc.date.none.fl_str_mv 2021
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/286791
https://api.elsevier.com/content/abstract/scopus_id/85111627268
url http://hdl.handle.net/10261/286791
https://api.elsevier.com/content/abstract/scopus_id/85111627268
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106380RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-094781-B-I00
Frontiers in cell and developmental biology
https://doi.org/10.3389/fcell.2021.673961
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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