Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)

Rodríguez García, Marcela; Bosch Serra, Dolors; Sauphanor, Benoit; Avilla Hernández, Jesús

Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)

The mechanisms associated with resistance of codling moth (Cydia pomonella (L.)) to organophosphate insecticides in pome fruit orchards have been shown to depend on the area. Our objectives were to evaluate the susceptibility of Spanish codling moth populations to chlorpyrifos-ethyl, azinphos-methyl...

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Detalhes bibliográficos
Autores:	Rodríguez García, Marcela, Bosch Serra, Dolors, Sauphanor, Benoit, Avilla Hernández, Jesús
Formato:	artículo
Estado:	Versión aceptada para publicación
Fecha de publicación:	2010
País:	España
Recursos:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:10459.1/67421
Acesso em linha:	https://doi.org/10.1603/EC09249 http://hdl.handle.net/10459.1/67421
Access Level:	acceso abierto
Palavra-chave:	Bioassay Detoxifying enzymes Organophosphates Resistance detection Arbres fruiters Control integrat de plagues Fruit trees Integrated control of agricultural pests

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spelling	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)Rodríguez García, MarcelaBosch Serra, DolorsSauphanor, BenoitAvilla Hernández, JesúsBioassayDetoxifying enzymesOrganophosphatesResistance detectionArbres fruitersControl integrat de plaguesFruit treesIntegrated control of agricultural pestsThe mechanisms associated with resistance of codling moth (Cydia pomonella (L.)) to organophosphate insecticides in pome fruit orchards have been shown to depend on the area. Our objectives were to evaluate the susceptibility of Spanish codling moth populations to chlorpyrifos-ethyl, azinphos-methyl and phosalone, and the activity of three enzymatic systems reported to be involved in resistance. Eleven field populations and a susceptible strain used as a reference were tested using a bioassay consisting in the topical application of a diagnostic concentration on post-diapausing larvae. The enzymatic activity of mixed-function oxidases (MFO), glutathione-S-transferases (GST) and esterases (EST) was measured in post-diapausing larvae and adults. A significant decrease in the efficacy of the organophosphates was observed for all field populations, though the decrease was smaller in the case of chlorpyrifos-ethyl. No differences between sexes were detected. In post-diapausing larvae, the activity of the three enzymatic systems was higher in all the field populations than in the susceptible one. The possible implication of EST in codling moth insecticide resistance is reported for the first time in European field populations. In adults, only MFO and GST were implicated. Codling moth resistance to organophosphates in Spanish populations must be taken into account in the implementation of anti-resistance strategies.This work was financed by grant AGL2004-05812/AGR.Entomological Society of America2019201920102019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1603/EC09249http://hdl.handle.net/10459.1/67421http://hdl.handle.net/10459.1/67421reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MEC//AGL2004-05812Versió postprint del document publicat a: https://doi.org/10.1603/EC09249Journal of Economic Entomology, 2010, vol. 103, num. 2, p. 482-491(c) Entomological Society of America, 2010info:eu-repo/semantics/openAccessoai:recercat.cat:10459.1/674212026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
title	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
spellingShingle	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae) Rodríguez García, Marcela Bioassay Detoxifying enzymes Organophosphates Resistance detection Arbres fruiters Control integrat de plagues Fruit trees Integrated control of agricultural pests
title_short	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
title_full	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
title_fullStr	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
title_full_unstemmed	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
title_sort	Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
dc.creator.none.fl_str_mv	Rodríguez García, Marcela Bosch Serra, Dolors Sauphanor, Benoit Avilla Hernández, Jesús
author	Rodríguez García, Marcela
author_facet	Rodríguez García, Marcela Bosch Serra, Dolors Sauphanor, Benoit Avilla Hernández, Jesús
author_role	author
author2	Bosch Serra, Dolors Sauphanor, Benoit Avilla Hernández, Jesús
author2_role	author author author
dc.subject.none.fl_str_mv	Bioassay Detoxifying enzymes Organophosphates Resistance detection Arbres fruiters Control integrat de plagues Fruit trees Integrated control of agricultural pests
topic	Bioassay Detoxifying enzymes Organophosphates Resistance detection Arbres fruiters Control integrat de plagues Fruit trees Integrated control of agricultural pests
description	The mechanisms associated with resistance of codling moth (Cydia pomonella (L.)) to organophosphate insecticides in pome fruit orchards have been shown to depend on the area. Our objectives were to evaluate the susceptibility of Spanish codling moth populations to chlorpyrifos-ethyl, azinphos-methyl and phosalone, and the activity of three enzymatic systems reported to be involved in resistance. Eleven field populations and a susceptible strain used as a reference were tested using a bioassay consisting in the topical application of a diagnostic concentration on post-diapausing larvae. The enzymatic activity of mixed-function oxidases (MFO), glutathione-S-transferases (GST) and esterases (EST) was measured in post-diapausing larvae and adults. A significant decrease in the efficacy of the organophosphates was observed for all field populations, though the decrease was smaller in the case of chlorpyrifos-ethyl. No differences between sexes were detected. In post-diapausing larvae, the activity of the three enzymatic systems was higher in all the field populations than in the susceptible one. The possible implication of EST in codling moth insecticide resistance is reported for the first time in European field populations. In adults, only MFO and GST were implicated. Codling moth resistance to organophosphates in Spanish populations must be taken into account in the implementation of anti-resistance strategies.
publishDate	2010
dc.date.none.fl_str_mv	2010 2019 2019 2019
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.1603/EC09249 http://hdl.handle.net/10459.1/67421 http://hdl.handle.net/10459.1/67421
url	https://doi.org/10.1603/EC09249 http://hdl.handle.net/10459.1/67421
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	info:eu-repo/grantAgreement/MEC//AGL2004-05812 Versió postprint del document publicat a: https://doi.org/10.1603/EC09249 Journal of Economic Entomology, 2010, vol. 103, num. 2, p. 482-491
dc.rights.none.fl_str_mv	(c) Entomological Society of America, 2010 info:eu-repo/semantics/openAccess
rights_invalid_str_mv	(c) Entomological Society of America, 2010
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Entomological Society of America
publisher.none.fl_str_mv	Entomological Society of America
dc.source.none.fl_str_mv	reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,812429

Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)

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