Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain
In 2020, a highly virulent PRRSV-1 strain emerged in Spain and rapidly spread across the country. The purpose of the present study was to test in a piglet model whether a commercial PRRSV-1 modified live vaccine was able to confer protection against strain R1, a representative of the emerging clade....
| Autores: | , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:321016 |
| Acesso em linha: | https://ddd.uab.cat/record/321016 https://dx.doi.org/urn:doi:10.1186/s40813-025-00423-y |
| Access Level: | acceso abierto |
| Palavra-chave: | Porcine reproductive and respiratory syndrome virus Virulence Vaccine Pig |
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Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strainCortey, Martí|||0000-0003-1126-3423Jiménez Farrerons, Marcel|||0000-0002-6602-7332Aguirre Molins, Laia|||0000-0003-3257-3965Sánchez Carvajal, Jose María|||0000-0003-0852-3765Gómez-Laguna, Jaime|||0000-0002-1787-5642Domingo Carreño, Ivan|||0009-0001-9927-9069Clilverd, Hepzibar|||0000-0002-4309-7506Marcos, M.Menjon, R.Von Berg, S.Mateu de Antonio, Enrique María|||0000-0003-2715-6032Porcine reproductive and respiratory syndrome virusVirulenceVaccinePigIn 2020, a highly virulent PRRSV-1 strain emerged in Spain and rapidly spread across the country. The purpose of the present study was to test in a piglet model whether a commercial PRRSV-1 modified live vaccine was able to confer protection against strain R1, a representative of the emerging clade. For that purpose, two groups of 26 piglets were either vaccinated intradermally or kept as controls; 42 days later, half of the animals in each group were intranasally challenged with the R1 strain. Then, animals were followed to assess the development of clinical signs (until 14 days post-challenge), lung lesions (10- and 35-days post-challenge), weight gains, viremia and nasal shedding and the immune response (anti PRRS virus nucleoprotein antibodies) by ELISA and virus specific-interferon-γ secreting cells by ELISPOT). Challenge of naïve pigs resulted in high fever (up to 41.9 °C), lethargy and severely retarded growth (0.748 kg/day). In contrast, vaccinated/challenged pigs had less fever and for a shorter period, lower clinical scores and a higher average daily weight gain (0.940 kg/day), comparable to the unchallenged animals. At 10 days-post challenge, in naïve animals on average 49.1% of the lung was pneumonic (range 8-81%) while in vaccinated animals the average was 15.7% (4-41%). Duration of viremia was reduced in vaccinated animals and after 14 days post-challenge, most were negative by RT-qPCR. In contrast, 50% of the naïve/challenged pigs remained viremic at 35 days post-challenge. Vaccination induced rapid seroconversion and challenge of naïve animals resulted in 100% of ELISA-positive pigs by day 14 post-challenge. Regarding the development of IFN-γ responses, for vaccinated animals the frequencies increased until day 35 post-vaccination. After challenge, in vaccinated pigs, the peak of the R1-specific IFN-γ response was reached at 14 days and then the viremia ceased, although nasal shedding persisted in some vaccinated animals. In the present trial, vaccination resulted in improved clinical course, better weight gain and reduced viremia. At the peak of the infection, lung lesions were reduced in most animals although some individuals still had extensive pneumonia. In summary, vaccination was shown to provide partial but significant protection against the highly virulent R1 strain. The online version contains supplementary material available at 10.1186/s40813-025-00423-y. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/321016https://dx.doi.org/urn:doi:10.1186/s40813-025-00423-yreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3210162026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain |
| title |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain |
| spellingShingle |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain Cortey, Martí|||0000-0003-1126-3423 Porcine reproductive and respiratory syndrome virus Virulence Vaccine Pig |
| title_short |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain |
| title_full |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain |
| title_fullStr |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain |
| title_full_unstemmed |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain |
| title_sort |
Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain |
| dc.creator.none.fl_str_mv |
Cortey, Martí|||0000-0003-1126-3423 Jiménez Farrerons, Marcel|||0000-0002-6602-7332 Aguirre Molins, Laia|||0000-0003-3257-3965 Sánchez Carvajal, Jose María|||0000-0003-0852-3765 Gómez-Laguna, Jaime|||0000-0002-1787-5642 Domingo Carreño, Ivan|||0009-0001-9927-9069 Clilverd, Hepzibar|||0000-0002-4309-7506 Marcos, M. Menjon, R. Von Berg, S. Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author |
Cortey, Martí|||0000-0003-1126-3423 |
| author_facet |
Cortey, Martí|||0000-0003-1126-3423 Jiménez Farrerons, Marcel|||0000-0002-6602-7332 Aguirre Molins, Laia|||0000-0003-3257-3965 Sánchez Carvajal, Jose María|||0000-0003-0852-3765 Gómez-Laguna, Jaime|||0000-0002-1787-5642 Domingo Carreño, Ivan|||0009-0001-9927-9069 Clilverd, Hepzibar|||0000-0002-4309-7506 Marcos, M. Menjon, R. Von Berg, S. Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author_role |
author |
| author2 |
Jiménez Farrerons, Marcel|||0000-0002-6602-7332 Aguirre Molins, Laia|||0000-0003-3257-3965 Sánchez Carvajal, Jose María|||0000-0003-0852-3765 Gómez-Laguna, Jaime|||0000-0002-1787-5642 Domingo Carreño, Ivan|||0009-0001-9927-9069 Clilverd, Hepzibar|||0000-0002-4309-7506 Marcos, M. Menjon, R. Von Berg, S. Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Porcine reproductive and respiratory syndrome virus Virulence Vaccine Pig |
| topic |
Porcine reproductive and respiratory syndrome virus Virulence Vaccine Pig |
| description |
In 2020, a highly virulent PRRSV-1 strain emerged in Spain and rapidly spread across the country. The purpose of the present study was to test in a piglet model whether a commercial PRRSV-1 modified live vaccine was able to confer protection against strain R1, a representative of the emerging clade. For that purpose, two groups of 26 piglets were either vaccinated intradermally or kept as controls; 42 days later, half of the animals in each group were intranasally challenged with the R1 strain. Then, animals were followed to assess the development of clinical signs (until 14 days post-challenge), lung lesions (10- and 35-days post-challenge), weight gains, viremia and nasal shedding and the immune response (anti PRRS virus nucleoprotein antibodies) by ELISA and virus specific-interferon-γ secreting cells by ELISPOT). Challenge of naïve pigs resulted in high fever (up to 41.9 °C), lethargy and severely retarded growth (0.748 kg/day). In contrast, vaccinated/challenged pigs had less fever and for a shorter period, lower clinical scores and a higher average daily weight gain (0.940 kg/day), comparable to the unchallenged animals. At 10 days-post challenge, in naïve animals on average 49.1% of the lung was pneumonic (range 8-81%) while in vaccinated animals the average was 15.7% (4-41%). Duration of viremia was reduced in vaccinated animals and after 14 days post-challenge, most were negative by RT-qPCR. In contrast, 50% of the naïve/challenged pigs remained viremic at 35 days post-challenge. Vaccination induced rapid seroconversion and challenge of naïve animals resulted in 100% of ELISA-positive pigs by day 14 post-challenge. Regarding the development of IFN-γ responses, for vaccinated animals the frequencies increased until day 35 post-vaccination. After challenge, in vaccinated pigs, the peak of the R1-specific IFN-γ response was reached at 14 days and then the viremia ceased, although nasal shedding persisted in some vaccinated animals. In the present trial, vaccination resulted in improved clinical course, better weight gain and reduced viremia. At the peak of the infection, lung lesions were reduced in most animals although some individuals still had extensive pneumonia. In summary, vaccination was shown to provide partial but significant protection against the highly virulent R1 strain. The online version contains supplementary material available at 10.1186/s40813-025-00423-y. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2 2025-01-01 2025 2025-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/321016 https://dx.doi.org/urn:doi:10.1186/s40813-025-00423-y |
| url |
https://ddd.uab.cat/record/321016 https://dx.doi.org/urn:doi:10.1186/s40813-025-00423-y |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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