Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica

[EN] Background: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are a...

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Autores: Ráez Bravo, Arián, Granados, José Enrique, Serrano, Emmanuel, Dellamaria, Debora, Casais, Rosa, Rossi, Luca, Puigdemont, Anna, Cano Manuel, Francisco Javier, Fandos, Paulino, Pérez, Jesús María, Espinosa Cerrato, José, Soriguer, Ramón C., Citterio, Carlo, López Olvera, Jorge Ramón
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/19865
Acceso en línea:https://hdl.handle.net/10612/19865
Access Level:acceso abierto
Palabra clave:Sanidad animal
Capra pyrenaica
Diagnostic
ELISA
Sarcoptes scabiei
Serum
3109 Ciencias Veterinarias
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
title Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
spellingShingle Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
Ráez Bravo, Arián
Sanidad animal
Capra pyrenaica
Diagnostic
ELISA
Sarcoptes scabiei
Serum
3109 Ciencias Veterinarias
title_short Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
title_full Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
title_fullStr Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
title_full_unstemmed Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
title_sort Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
dc.creator.none.fl_str_mv Ráez Bravo, Arián
Granados, José Enrique
Serrano, Emmanuel
Dellamaria, Debora
Casais, Rosa
Rossi, Luca
Puigdemont, Anna
Cano Manuel, Francisco Javier
Fandos, Paulino
Pérez, Jesús María
Espinosa Cerrato, José
Soriguer, Ramón C.
Citterio, Carlo
López Olvera, Jorge Ramón
author Ráez Bravo, Arián
author_facet Ráez Bravo, Arián
Granados, José Enrique
Serrano, Emmanuel
Dellamaria, Debora
Casais, Rosa
Rossi, Luca
Puigdemont, Anna
Cano Manuel, Francisco Javier
Fandos, Paulino
Pérez, Jesús María
Espinosa Cerrato, José
Soriguer, Ramón C.
Citterio, Carlo
López Olvera, Jorge Ramón
author_role author
author2 Granados, José Enrique
Serrano, Emmanuel
Dellamaria, Debora
Casais, Rosa
Rossi, Luca
Puigdemont, Anna
Cano Manuel, Francisco Javier
Fandos, Paulino
Pérez, Jesús María
Espinosa Cerrato, José
Soriguer, Ramón C.
Citterio, Carlo
López Olvera, Jorge Ramón
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Capra pyrenaica
Diagnostic
ELISA
Sarcoptes scabiei
Serum
3109 Ciencias Veterinarias
topic Sanidad animal
Capra pyrenaica
Diagnostic
ELISA
Sarcoptes scabiei
Serum
3109 Ciencias Veterinarias
description [EN] Background: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are available for this disease, particularly when there are low mite numbers and mild or no clinical signs. In this study, three enzyme-linked immunosorbent assays (ELISA) developed for dog (ELISA A), Cantabrian chamois (Rupicapra pyrenaica parva) (ELISA B) and Alpine chamois (Rupicapra rupicapra) (ELISA C), were evaluated to detect specific antibodies (IgG) to sarcoptic mange in Iberian ibex sera. Methods: Serum samples from 131 Iberian ibexes (86 healthy and 45 scabietic) were collected from 2005 to 2012 in the Sierra Nevada Natural and National Parks (southern Spain). Based on visual inspection, ibexes were classified into one of three categories, namely healthy (without scabietic compatible lesions), mildly affected (skin lesions over less than 50 % of the body surface) and severely affected (skin lesions over more than 50 % of the body surface). The optimal cut-off point, specificity, sensitivity and the area under the curve (AUC) were calculated, and the agreement between tests was determined. Moreover, differences in the optical density (OD) related to scabies severity have been evaluated for the best test. Results: ELISA C showed better performance than the two other tests, reaching higher values of sensitivity (93.0 %) and specificity (93.5 %) against the visual estimation of the percentage of affected skin, chosen as the gold standard. Significantly higher concentrations of specific antibodies were observed with this test in the mildly and severely infested ibexes than in healthy ones. Conclusions: Our results revealed that ELISA C was an optimal test to diagnose sarcoptic mange in the Iberian ibex. Further studies characterizing immune response during the course of the disease, including spontaneous or drug induced recovery, should follow in order to better understand sarcoptic mange in Iberian ibex populations
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10612/19865
url https://hdl.handle.net/10612/19865
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ MINECO/ Programa Nacional de Investigación Fundamental/CGL2012-40043-C02-02
info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ BES-2013-063931
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaicaRáez Bravo, AriánGranados, José EnriqueSerrano, EmmanuelDellamaria, DeboraCasais, RosaRossi, LucaPuigdemont, AnnaCano Manuel, Francisco JavierFandos, PaulinoPérez, Jesús MaríaEspinosa Cerrato, JoséSoriguer, Ramón C.Citterio, CarloLópez Olvera, Jorge RamónSanidad animalCapra pyrenaicaDiagnosticELISASarcoptes scabieiSerum3109 Ciencias Veterinarias[EN] Background: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are available for this disease, particularly when there are low mite numbers and mild or no clinical signs. In this study, three enzyme-linked immunosorbent assays (ELISA) developed for dog (ELISA A), Cantabrian chamois (Rupicapra pyrenaica parva) (ELISA B) and Alpine chamois (Rupicapra rupicapra) (ELISA C), were evaluated to detect specific antibodies (IgG) to sarcoptic mange in Iberian ibex sera. Methods: Serum samples from 131 Iberian ibexes (86 healthy and 45 scabietic) were collected from 2005 to 2012 in the Sierra Nevada Natural and National Parks (southern Spain). Based on visual inspection, ibexes were classified into one of three categories, namely healthy (without scabietic compatible lesions), mildly affected (skin lesions over less than 50 % of the body surface) and severely affected (skin lesions over more than 50 % of the body surface). The optimal cut-off point, specificity, sensitivity and the area under the curve (AUC) were calculated, and the agreement between tests was determined. Moreover, differences in the optical density (OD) related to scabies severity have been evaluated for the best test. Results: ELISA C showed better performance than the two other tests, reaching higher values of sensitivity (93.0 %) and specificity (93.5 %) against the visual estimation of the percentage of affected skin, chosen as the gold standard. Significantly higher concentrations of specific antibodies were observed with this test in the mildly and severely infested ibexes than in healthy ones. Conclusions: Our results revealed that ELISA C was an optimal test to diagnose sarcoptic mange in the Iberian ibex. Further studies characterizing immune response during the course of the disease, including spontaneous or drug induced recovery, should follow in order to better understand sarcoptic mange in Iberian ibex populationsSIThe Consejería de Medio Ambiente y Ordenación del Territorio of the Junta de Andalucía supported sample collection and conservation through the projects 676/2006/A/00, 1571/2007/M/00, 173/2009/M/00 and 861/2011/M/ 00, as did the PAIDI Research Group RNM18 from the Junta de Andalucía. The Ministerio de Economía y Competitividad of the Spanish Government funded the study through the research projects CGL2012-40043-C02-02 and CGL2016-80543-P, and a predoctoral grant (BES-2013-063931) to JE. ES was supported by the Fundação para a Ciência ea Tecnologia (Portugal), through the postdoctoral program (SFRH/BPD/96637/2013), and the University of Aveiro (Department of Biology) and FCT/MEC (CESAM RU UID/AMB/50017), co-financed by the FEDER within the PT2020 Partnership Agreement. LR, DD and CC were supported by the Italian National PRIN Program “Genomics and host-pathogen interaction: a study model in the One-Health perspective”BMCSanidad AnimalFacultad de Veterinaria2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/10612/19865reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/ MINECO/ Programa Nacional de Investigación Fundamental/CGL2012-40043-C02-02 info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ BES-2013-063931http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/198652026-06-24T12:43:27Z
score 15,300724

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