Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The stud...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/306896 |
| Acesso em linha: | http://hdl.handle.net/10261/306896 https://api.elsevier.com/content/abstract/scopus_id/85124612095 |
| Access Level: | acceso abierto |
| Palavra-chave: | MALDI-TOF MS Carbapenemases enzymes Clinical microbiology Imipenem Resistance detection |
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Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online SoftwareGato, EvaAnantharajah, AhalieyahArroyo, Manuel J.Artacho-Reinoso, María JoséCaballero, Juan de DiosCandela, AnaChudějová, KateřinaConstanso, Ignacio PedroElías-López, CristinaFernández, JavierJiménez, JesúsLumbreras, PilarMéndez, GemaMulet, XavierPérez-Palacios, PatriciaRodríguez-Sánchez, BelénCantón, RafaelHrabák, JaroslavMancera, LuisMartínez-Martínez, LuisOliver, AntonioPascual, ÁlvaroVerroken, AlexiaBou, GermánOviaño, MarinaMALDI-TOF MSCarbapenemases enzymesClinical microbiologyImipenemResistance detectionIn this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: "software design," "intercenter evaluation," and "clinical validation." First, a standardized procedure with an online software for data analysis was designed. Carbapenem resistance was detected by measuring imipenem hydrolysis and the results were automatically interpreted using the Clover MS data analysis software (Clover BioSoft, Spain). Second, a series of 74 genotypically characterized Enterobacterales (46 carbapenemase-producers and 28 non carbapenemase-producers) were analyzed in 8 international centers to ensure the reproducibility of the method. Finally, the methodology was evaluated independently in all centers during a 2-month period and results were compared with the reference standard for carbapenemase detection used in each center. The overall agreement rate relative to the reference method for carbapenemase resistance detection in clinical samples was 92.5%. The sensitivity was 93.9% and the specificity, 100%. Results were obtained within 60 min and accuracy ranged from 83.3 to 100% among the different centers. Further, our results demonstrate that MALDI-TOF MS is an outstanding tool for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories. The use of a simple in-house procedure with online software allows routine screening of carbapenemases in diagnostics, thereby facilitating early and appropriate antimicrobial therapy.This work was supported by the Fondo de Investigación Sanitaria (grant numbers PI20/00686 to MO and PI18/00860 to GB) integrated in the Plan Nacional de I+D and funded by the Instituto de Salud Carlos III (ISCIII). The results of this work have been funded by the Spanish Network of Research in Infectious Diseases (REIPI), N° RD16/0016/0006 and RD16/0016/0011, integrated in the National Plan for Scientific Research, Development and Technological Innovation 2013–2016, and funded by the ISCIII-General Subdirection of Assesment and Promotion of the Research – European Regional Development Fund (FEDER) “A way of making Europe.”Peer reviewedFrontiers MediaInstituto de Salud Carlos IIIRed Española de Investigación en Patología InfecciosaEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/306896https://api.elsevier.com/content/abstract/scopus_id/85124612095reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3389/fmicb.2021.789731Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3068962026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
| title |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
| spellingShingle |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software Gato, Eva MALDI-TOF MS Carbapenemases enzymes Clinical microbiology Imipenem Resistance detection |
| title_short |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
| title_full |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
| title_fullStr |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
| title_full_unstemmed |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
| title_sort |
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
| dc.creator.none.fl_str_mv |
Gato, Eva Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho-Reinoso, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías-López, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina |
| author |
Gato, Eva |
| author_facet |
Gato, Eva Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho-Reinoso, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías-López, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina |
| author_role |
author |
| author2 |
Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho-Reinoso, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías-López, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Salud Carlos III Red Española de Investigación en Patología Infecciosa European Commission Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
MALDI-TOF MS Carbapenemases enzymes Clinical microbiology Imipenem Resistance detection |
| topic |
MALDI-TOF MS Carbapenemases enzymes Clinical microbiology Imipenem Resistance detection |
| description |
In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: "software design," "intercenter evaluation," and "clinical validation." First, a standardized procedure with an online software for data analysis was designed. Carbapenem resistance was detected by measuring imipenem hydrolysis and the results were automatically interpreted using the Clover MS data analysis software (Clover BioSoft, Spain). Second, a series of 74 genotypically characterized Enterobacterales (46 carbapenemase-producers and 28 non carbapenemase-producers) were analyzed in 8 international centers to ensure the reproducibility of the method. Finally, the methodology was evaluated independently in all centers during a 2-month period and results were compared with the reference standard for carbapenemase detection used in each center. The overall agreement rate relative to the reference method for carbapenemase resistance detection in clinical samples was 92.5%. The sensitivity was 93.9% and the specificity, 100%. Results were obtained within 60 min and accuracy ranged from 83.3 to 100% among the different centers. Further, our results demonstrate that MALDI-TOF MS is an outstanding tool for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories. The use of a simple in-house procedure with online software allows routine screening of carbapenemases in diagnostics, thereby facilitating early and appropriate antimicrobial therapy. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/306896 https://api.elsevier.com/content/abstract/scopus_id/85124612095 |
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http://hdl.handle.net/10261/306896 https://api.elsevier.com/content/abstract/scopus_id/85124612095 |
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Inglés |
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Inglés |
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https://doi.org/10.3389/fmicb.2021.789731 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Frontiers Media |
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