Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions

Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, curre...

ver descrição completa

Detalhes bibliográficos
Autores: Lukovic, Dunja, Díez Lloret, Andrea, Stojkovic, Petra, Rodríguez Martínez, Daniel, Rodríguez Jiménez, Francisco Javier, González Rodríguez, P., López Barneo, José, Moreno Manzano, Victoria, Stojkovic, Miodrag, Bhattacharya, Shomi S., Erceg, Slaven
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/81101
Acesso em linha:https://hdl.handle.net/11441/81101
https://doi.org/10.1002/sctm.16-0371
Access Level:acceso abierto
Palavra-chave:Cellular therapy
Clinical translation
Differentiation
Embryonic stem cells
Induced pluripotent stem cells
Neural differentiation
Pluripotent stem cells
id ES_be2a6c476a8aaff843e08f7d468ecbe2
oai_identifier_str oai:idus.us.es:11441/81101
network_acronym_str ES
network_name_str España
repository_id_str
spelling Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditionsLukovic, DunjaDíez Lloret, AndreaStojkovic, PetraRodríguez Martínez, DanielRodríguez Jiménez, Francisco JavierGonzález Rodríguez, P.López Barneo, JoséMoreno Manzano, VictoriaStojkovic, MiodragBhattacharya, Shomi S.Erceg, SlavenCellular therapyClinical translationDifferentiationEmbryonic stem cellsInduced pluripotent stem cellsNeural differentiationPluripotent stem cellsNeural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic.WileyFisiología Médica y Biofísica2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/81101https://doi.org/10.1002/sctm.16-0371reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésStem Cells Translational Medicine, 6 (4), 1217-1226.https://doi.org/10.1002/sctm.16-0371info:eu-repo/semantics/openAccessoai:idus.us.es:11441/811012026-06-17T12:51:07Z
dc.title.none.fl_str_mv Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
title Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
spellingShingle Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
Lukovic, Dunja
Cellular therapy
Clinical translation
Differentiation
Embryonic stem cells
Induced pluripotent stem cells
Neural differentiation
Pluripotent stem cells
title_short Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
title_full Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
title_fullStr Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
title_full_unstemmed Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
title_sort Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
dc.creator.none.fl_str_mv Lukovic, Dunja
Díez Lloret, Andrea
Stojkovic, Petra
Rodríguez Martínez, Daniel
Rodríguez Jiménez, Francisco Javier
González Rodríguez, P.
López Barneo, José
Moreno Manzano, Victoria
Stojkovic, Miodrag
Bhattacharya, Shomi S.
Erceg, Slaven
author Lukovic, Dunja
author_facet Lukovic, Dunja
Díez Lloret, Andrea
Stojkovic, Petra
Rodríguez Martínez, Daniel
Rodríguez Jiménez, Francisco Javier
González Rodríguez, P.
López Barneo, José
Moreno Manzano, Victoria
Stojkovic, Miodrag
Bhattacharya, Shomi S.
Erceg, Slaven
author_role author
author2 Díez Lloret, Andrea
Stojkovic, Petra
Rodríguez Martínez, Daniel
Rodríguez Jiménez, Francisco Javier
González Rodríguez, P.
López Barneo, José
Moreno Manzano, Victoria
Stojkovic, Miodrag
Bhattacharya, Shomi S.
Erceg, Slaven
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fisiología Médica y Biofísica
dc.subject.none.fl_str_mv Cellular therapy
Clinical translation
Differentiation
Embryonic stem cells
Induced pluripotent stem cells
Neural differentiation
Pluripotent stem cells
topic Cellular therapy
Clinical translation
Differentiation
Embryonic stem cells
Induced pluripotent stem cells
Neural differentiation
Pluripotent stem cells
description Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/81101
https://doi.org/10.1002/sctm.16-0371
url https://hdl.handle.net/11441/81101
https://doi.org/10.1002/sctm.16-0371
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Stem Cells Translational Medicine, 6 (4), 1217-1226.
https://doi.org/10.1002/sctm.16-0371
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418262605856768
score 15,300724

Registos relacionados