Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus

© 2020 by the authors.

Detalles Bibliográficos
Autores: Gilannejad, Neda, Heras, Verónica de las, Martos-Sitcha, Juan Antonio, Moyano, Francisco Javier, Yúfera, Manuel, Martínez-Rodríguez, Gonzalo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/212509
Acceso en línea:http://hdl.handle.net/10261/212509
Access Level:acceso abierto
Palabra clave:Chelon labrosus
Digestive function
Enzyme activity
Gene expression
Ontogeny
Somatotropic factors
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spelling Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosusGilannejad, NedaHeras, Verónica de lasMartos-Sitcha, Juan AntonioMoyano, Francisco JavierYúfera, ManuelMartínez-Rodríguez, GonzaloChelon labrosusDigestive functionEnzyme activityGene expressionOntogenySomatotropic factors© 2020 by the authors.Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of key digestive enzymes was measured from three until 90 days post hatch (dph). Expression of genes involved in digestion of proteins (try2, ctr, pga2, and atp4a), carbohydrates (amy2a), and lipids (cel and pla2g1b), together with two somatotropic factors (gh and igf1) were also quantified. No chymotrypsin or pepsin activities were detected. While specific activity of trypsin and lipase were high during the first 30 dph and declined afterward, amylase activity was low until 57 dph and increased significantly beyond that point. Expression of try2, ctr, amy2a, and cel increased continuously along development, and showed a peak at the end of metamorphosis. Expression of pla2g1b, pga2 and atp4a increased until the middle of metamorphosis and decreased afterwars. Most of these trends contrast the usual patterns in carnivorous species and highlight the transition from larvae, with high protein requirements, to post-larvae/juvenile stages, with omnivorous/detritivorous feeding preferences. Somatotropic genes, gh and igf1, showed approximately inverse expression patterns, suggesting the establishment of the Gh/Igf1 axis from 50 dph.The present study was funded by the project “Optimization of the maturation and spawning processes in the mullet Chelon labrosus” from the Spanish Ministry of Education and European Social Fund.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Educación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/212509reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/ani10050874Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2125092026-05-22T06:33:51Z
dc.title.none.fl_str_mv Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
title Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
spellingShingle Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
Gilannejad, Neda
Chelon labrosus
Digestive function
Enzyme activity
Gene expression
Ontogeny
Somatotropic factors
title_short Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
title_full Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
title_fullStr Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
title_full_unstemmed Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
title_sort Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
dc.creator.none.fl_str_mv Gilannejad, Neda
Heras, Verónica de las
Martos-Sitcha, Juan Antonio
Moyano, Francisco Javier
Yúfera, Manuel
Martínez-Rodríguez, Gonzalo
author Gilannejad, Neda
author_facet Gilannejad, Neda
Heras, Verónica de las
Martos-Sitcha, Juan Antonio
Moyano, Francisco Javier
Yúfera, Manuel
Martínez-Rodríguez, Gonzalo
author_role author
author2 Heras, Verónica de las
Martos-Sitcha, Juan Antonio
Moyano, Francisco Javier
Yúfera, Manuel
Martínez-Rodríguez, Gonzalo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chelon labrosus
Digestive function
Enzyme activity
Gene expression
Ontogeny
Somatotropic factors
topic Chelon labrosus
Digestive function
Enzyme activity
Gene expression
Ontogeny
Somatotropic factors
description © 2020 by the authors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/212509
url http://hdl.handle.net/10261/212509
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/ani10050874
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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