Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses

12 Pág.

Detalhes bibliográficos
Autores: Rodríguez Pulido, Miguel Ramón, Calvo Pinilla, Eva María, Polo, Miryam, Saiz Calahorra, Juan Carlos, Fernández González, Raúl, Pericuesta Camacho, Eva, Gutiérrez-Adán, Alfonso, Sobrino Castelló, Francisco, Martín-Acebes, M. A., Sáiz, Margarita
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330229
Acesso em linha:http://hdl.handle.net/10261/330229
https://api.elsevier.com/content/abstract/scopus_id/85152628012
Access Level:acceso abierto
Palavra-chave:COVID-19
RNA-based therapy
SARS-CoV-2
Antiviral immunity
Coronaviruses
Foot-and-mouth disease virus (FMDV)
Non-coding RNA
Type-I IFN
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oai_identifier_str oai:digital.csic.es:10261/330229
network_acronym_str ES
network_name_str España
repository_id_str
spelling Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronavirusesRodríguez Pulido, Miguel RamónCalvo Pinilla, Eva MaríaPolo, MiryamSaiz Calahorra, Juan CarlosFernández González, RaúlPericuesta Camacho, EvaGutiérrez-Adán, AlfonsoSobrino Castelló, FranciscoMartín-Acebes, M. A.Sáiz, MargaritaCOVID-19RNA-based therapySARS-CoV-2Antiviral immunityCoronavirusesFoot-and-mouth disease virus (FMDV)Non-coding RNAType-I IFN12 Pág.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of a potentially severe respiratory disease, the coronavirus disease 2019 (COVID-19), an ongoing pandemic with limited therapeutic options. Here, we assessed the anti-coronavirus activity of synthetic RNAs mimicking specific domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs). These molecules are known to exert broad-spectrum antiviral activity in cell culture, mice and pigs effectively triggering the host innate immune response. The ncRNAs showed potent antiviral activity against SARS-CoV-2 after transfection in human intestinal Caco-2 and lung epithelium Calu-3 2B4 cells. When the in vivo efficacy of the FMDV ncRNAs was assessed in K18-hACE2 mice, administration of naked ncRNA before intranasal SARS-CoV-2 infection significantly decreased the viral load and the levels of pro-inflammatory cytokines in the lungs compared with untreated infected mice. The ncRNAs were also highly efficacious when assayed against common human HCoV-229E and porcine transmissible gastroenteritis virus (TGEV) in hepatocyte-derived Huh-7 and swine testis ST cells, respectively. These results are a proof of concept of the pan-coronavirus antiviral activity of the FMDV ncRNAs including human and animal divergent coronaviruses and potentially enhance our ability to fight future emerging variants.This research work was funded by the European Commission – NextGenerationEU (Regulation EU 2020/2094), through CSIC´s Global Health Platform (PTI Salud Global), grants SGL 2103051 (to MS and FS) and SGL2103053 (to MM-A). It was further supported by the Spanish Ministry of Science and Innovation, grant PID2020-113184RB-C21 (to FS and MS) and by grant S018/BAA-4370 (co-financed by the Autonomous Community of Madrid and EC FEDER funds, to FS). MP was the recipient of a scholarship from the CSIC´s JAE Intro program.Peer reviewedFrontiers MediaEuropean CommissionCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Ministerio de Ciencia e Innovación (España)CSIC - Plataforma Temática Interdisciplinar del CSIC Salud Global (PTI Salud Global)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas (España)Rodríguez Pulido, Miguel Ramón [0000-0003-2101-8974]Calvo Pinilla, Eva María [0000-0002-4667-4081]Polo, Miryam [0000-0002-5222-2313]Saiz Calahorra, Juan Carlos [0000-0001-8269-5544]Fernández González, Raúl [0000-0003-1989-2945]Pericuesta Camacho, Eva [0000-0001-9387-3270]Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]Sobrino, F. [0000-0003-4968-8291]Martín-Acebes, M. A. [0000-0001-6015-3613]Sáiz, Margarita [0000-0003-3893-7926]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/330229https://api.elsevier.com/content/abstract/scopus_id/85152628012reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113184RB-C21S2018/BAA-4370/PLATESA2-CMFrontiers in immunologyhttps://doi.org/10.3389/fimmu.2023.1166725Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3302292026-05-22T06:33:51Z
dc.title.none.fl_str_mv Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
title Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
spellingShingle Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
Rodríguez Pulido, Miguel Ramón
COVID-19
RNA-based therapy
SARS-CoV-2
Antiviral immunity
Coronaviruses
Foot-and-mouth disease virus (FMDV)
Non-coding RNA
Type-I IFN
title_short Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
title_full Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
title_fullStr Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
title_full_unstemmed Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
title_sort Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
dc.creator.none.fl_str_mv Rodríguez Pulido, Miguel Ramón
Calvo Pinilla, Eva María
Polo, Miryam
Saiz Calahorra, Juan Carlos
Fernández González, Raúl
Pericuesta Camacho, Eva
Gutiérrez-Adán, Alfonso
Sobrino Castelló, Francisco
Martín-Acebes, M. A.
Sáiz, Margarita
author Rodríguez Pulido, Miguel Ramón
author_facet Rodríguez Pulido, Miguel Ramón
Calvo Pinilla, Eva María
Polo, Miryam
Saiz Calahorra, Juan Carlos
Fernández González, Raúl
Pericuesta Camacho, Eva
Gutiérrez-Adán, Alfonso
Sobrino Castelló, Francisco
Martín-Acebes, M. A.
Sáiz, Margarita
author_role author
author2 Calvo Pinilla, Eva María
Polo, Miryam
Saiz Calahorra, Juan Carlos
Fernández González, Raúl
Pericuesta Camacho, Eva
Gutiérrez-Adán, Alfonso
Sobrino Castelló, Francisco
Martín-Acebes, M. A.
Sáiz, Margarita
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Ministerio de Ciencia e Innovación (España)
CSIC - Plataforma Temática Interdisciplinar del CSIC Salud Global (PTI Salud Global)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas (España)
Rodríguez Pulido, Miguel Ramón [0000-0003-2101-8974]
Calvo Pinilla, Eva María [0000-0002-4667-4081]
Polo, Miryam [0000-0002-5222-2313]
Saiz Calahorra, Juan Carlos [0000-0001-8269-5544]
Fernández González, Raúl [0000-0003-1989-2945]
Pericuesta Camacho, Eva [0000-0001-9387-3270]
Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]
Sobrino, F. [0000-0003-4968-8291]
Martín-Acebes, M. A. [0000-0001-6015-3613]
Sáiz, Margarita [0000-0003-3893-7926]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv COVID-19
RNA-based therapy
SARS-CoV-2
Antiviral immunity
Coronaviruses
Foot-and-mouth disease virus (FMDV)
Non-coding RNA
Type-I IFN
topic COVID-19
RNA-based therapy
SARS-CoV-2
Antiviral immunity
Coronaviruses
Foot-and-mouth disease virus (FMDV)
Non-coding RNA
Type-I IFN
description 12 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330229
https://api.elsevier.com/content/abstract/scopus_id/85152628012
url http://hdl.handle.net/10261/330229
https://api.elsevier.com/content/abstract/scopus_id/85152628012
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113184RB-C21
S2018/BAA-4370/PLATESA2-CM
Frontiers in immunology
https://doi.org/10.3389/fimmu.2023.1166725
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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