Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota

Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the mcr-1 gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effe...

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Autores: Miguela Villoldo, Pedro, Moreno, Miguel A., Rebollada Merino, Agustín Miguel, Rodríguez Bertos, Antonio Manuel, Hernández, Marta, Rodríguez Lázaro, David, Gallardo, Alejandro, Quesada, Alberto, Goyache Goñi, Joaquín, Ugarte Ruiz, María, Domínguez Rodríguez, Lucas José
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7125
Acceso en línea:https://hdl.handle.net/20.500.14352/7125
Access Level:acceso abierto
Palabra clave:colistin
mcr-1
Salmonella
in vivo assay
real-time PCR quantification
microbiota
chicks
Avicultura
Farmacología veterinaria
3109.08 Farmacología
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network_acronym_str ES
network_name_str España
repository_id_str
spelling Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal MicrobiotaMiguela Villoldo, PedroMoreno, Miguel A.Rebollada Merino, Agustín MiguelRodríguez Bertos, Antonio ManuelHernández, MartaRodríguez Lázaro, DavidGallardo, AlejandroQuesada, AlbertoGoyache Goñi, JoaquínUgarte Ruiz, MaríaDomínguez Rodríguez, Lucas Josécolistinmcr-1Salmonellain vivo assayreal-time PCR quantificationmicrobiotachicksAviculturaFarmacología veterinaria3109.08 FarmacologíaColistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the mcr-1 gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of mcr-1 genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with mcr-1-carrying Salmonella (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals through drinking water (from day 8 to day 15). Two quantitative PCR assays were performed to compare the amount of Salmonella and mcr-1 that were present in the caecal samples. We observed that levels of mcr-1 were higher in G3/G4 animals, especially G4, due to the spread of mcr-1-carrying Salmonella. On day 21, Salmonella levels decreased in G4, reaching similar values as those for G3, but mcr-1 levels remained significantly higher, suggesting that colistin may accelerate the spreading process when mcr-1-carrying bacteria reach the gut.MPDIUniversidad Complutense de Madrid20212021-06-0520212021-06-05journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/7125reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/71252026-06-02T12:44:21Z
dc.title.none.fl_str_mv Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
title Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
spellingShingle Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
Miguela Villoldo, Pedro
colistin
mcr-1
Salmonella
in vivo assay
real-time PCR quantification
microbiota
chicks
Avicultura
Farmacología veterinaria
3109.08 Farmacología
title_short Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
title_full Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
title_fullStr Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
title_full_unstemmed Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
title_sort Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
dc.creator.none.fl_str_mv Miguela Villoldo, Pedro
Moreno, Miguel A.
Rebollada Merino, Agustín Miguel
Rodríguez Bertos, Antonio Manuel
Hernández, Marta
Rodríguez Lázaro, David
Gallardo, Alejandro
Quesada, Alberto
Goyache Goñi, Joaquín
Ugarte Ruiz, María
Domínguez Rodríguez, Lucas José
author Miguela Villoldo, Pedro
author_facet Miguela Villoldo, Pedro
Moreno, Miguel A.
Rebollada Merino, Agustín Miguel
Rodríguez Bertos, Antonio Manuel
Hernández, Marta
Rodríguez Lázaro, David
Gallardo, Alejandro
Quesada, Alberto
Goyache Goñi, Joaquín
Ugarte Ruiz, María
Domínguez Rodríguez, Lucas José
author_role author
author2 Moreno, Miguel A.
Rebollada Merino, Agustín Miguel
Rodríguez Bertos, Antonio Manuel
Hernández, Marta
Rodríguez Lázaro, David
Gallardo, Alejandro
Quesada, Alberto
Goyache Goñi, Joaquín
Ugarte Ruiz, María
Domínguez Rodríguez, Lucas José
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv colistin
mcr-1
Salmonella
in vivo assay
real-time PCR quantification
microbiota
chicks
Avicultura
Farmacología veterinaria
3109.08 Farmacología
topic colistin
mcr-1
Salmonella
in vivo assay
real-time PCR quantification
microbiota
chicks
Avicultura
Farmacología veterinaria
3109.08 Farmacología
description Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the mcr-1 gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of mcr-1 genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with mcr-1-carrying Salmonella (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals through drinking water (from day 8 to day 15). Two quantitative PCR assays were performed to compare the amount of Salmonella and mcr-1 that were present in the caecal samples. We observed that levels of mcr-1 were higher in G3/G4 animals, especially G4, due to the spread of mcr-1-carrying Salmonella. On day 21, Salmonella levels decreased in G4, reaching similar values as those for G3, but mcr-1 levels remained significantly higher, suggesting that colistin may accelerate the spreading process when mcr-1-carrying bacteria reach the gut.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-06-05
2021
2021-06-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/7125
url https://hdl.handle.net/20.500.14352/7125
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MPDI
publisher.none.fl_str_mv MPDI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418247858683904
score 15.300724

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