Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol

Contaminants of emerging concern (CECs) are a class of chemicals that can spread throughout the environment and may cause adverse biological and ecological effects. While there are many different classes of CECs, one of the most well documented in the aquatic environment are pharmaceutical drugs, su...

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Autores: Pretti, Carlo, Aretini, Paolo, Lessi, Francesca, Freitas, Rosa, Barata Martí, Carlos, De Marchi, Lucia, Cuccaro, Alessia, Oliva, Matteo, Meucci, Valentina, Baratti, Mariella
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/286380
Acceso en línea:http://hdl.handle.net/10261/286380
https://api.elsevier.com/content/abstract/scopus_id/85144810709
Access Level:acceso abierto
Palabra clave:RNA-Seq
17 α-ethinylestradiol
Biochemistry
EE2
Mytilus galloprovincialis
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spelling Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiolPretti, CarloAretini, PaoloLessi, FrancescaFreitas, RosaBarata Martí, CarlosDe Marchi, LuciaCuccaro, AlessiaOliva, MatteoMeucci, ValentinaBaratti, MariellaRNA-Seq17 α-ethinylestradiolBiochemistryEE2Mytilus galloprovincialisContaminants of emerging concern (CECs) are a class of chemicals that can spread throughout the environment and may cause adverse biological and ecological effects. While there are many different classes of CECs, one of the most well documented in the aquatic environment are pharmaceutical drugs, such as natural and synthetic estrogens. In particular, the widespread presence of the synthetic estrogen 17 α-Ethinylestradiol (EE2) in water may lead to bioaccumulation in sediment and biota. EE2 is the primary component in contraceptive pills, and is a derivative of the natural hormone estradiol (E2). In this study, the mussel Mytilus galloprovincialis was exposed to EE2 in a semi-static and time-dependent experiment, for a total exposure period of 28 days. Biochemical and transcriptomics analyses were performed on mussel digestive glands after exposure for 14 (T14) and 28 (T28) days. Metabolic and DNA impairments, as well as activation of antioxidant and biotransformation enzymes activation, were detected in T28 exposed mussels. RNA-Seq analysis showed significant differential expression of 160 (T14 compared to controls), 33 (T28 compared to controls) and 79 (T14 compared to T28) genes. Signs of stress after EE2 treatment included up-regulation of gene/proteins involved with immune function, lipid transport, and metabolic and antibacterial properties. This study elucidates the underlying mechanisms of EE2 in a filter feeding organisms to elucidate the effects of this human pharmaceutical on aquatic biota.Alessia Cuccaro received a PhD grant (PD/BD/150609/2020) given by the National Funds through the Portuguese Science Foundation (Fundação para a Ciência e a Tecnologia, FCT). Lucia De Marchi (CDL-CTTRI-17-ARH/2021) received an assistant researchers’ contract in the scope of BISPECIAl Project (POCI-01-0145-FEDER-028425). This work was also financially supported by the project BISPECIAl: BIvalveS under Polluted Environment and ClImate chAnge (POCI-01-0145-FEDER- 028425) funded by FEDER, through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI), and by the University of Pisa through institutional funds “Fondi di Ateneo UNIPI”.Peer reviewedElsevier0000-0003-4949-2088Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/286380https://api.elsevier.com/content/abstract/scopus_id/85144810709reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésAquatic toxicology (Amsterdam, Netherlands)https://doi.org/10.1016/j.aquatox.2022.106376Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2863802026-05-22T06:33:51Z
dc.title.none.fl_str_mv Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
title Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
spellingShingle Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
Pretti, Carlo
RNA-Seq
17 α-ethinylestradiol
Biochemistry
EE2
Mytilus galloprovincialis
title_short Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
title_full Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
title_fullStr Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
title_full_unstemmed Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
title_sort Gene expression and biochemical patterns in the digestive gland of the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to 17α-ethinylestradiol
dc.creator.none.fl_str_mv Pretti, Carlo
Aretini, Paolo
Lessi, Francesca
Freitas, Rosa
Barata Martí, Carlos
De Marchi, Lucia
Cuccaro, Alessia
Oliva, Matteo
Meucci, Valentina
Baratti, Mariella
author Pretti, Carlo
author_facet Pretti, Carlo
Aretini, Paolo
Lessi, Francesca
Freitas, Rosa
Barata Martí, Carlos
De Marchi, Lucia
Cuccaro, Alessia
Oliva, Matteo
Meucci, Valentina
Baratti, Mariella
author_role author
author2 Aretini, Paolo
Lessi, Francesca
Freitas, Rosa
Barata Martí, Carlos
De Marchi, Lucia
Cuccaro, Alessia
Oliva, Matteo
Meucci, Valentina
Baratti, Mariella
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv 0000-0003-4949-2088
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv RNA-Seq
17 α-ethinylestradiol
Biochemistry
EE2
Mytilus galloprovincialis
topic RNA-Seq
17 α-ethinylestradiol
Biochemistry
EE2
Mytilus galloprovincialis
description Contaminants of emerging concern (CECs) are a class of chemicals that can spread throughout the environment and may cause adverse biological and ecological effects. While there are many different classes of CECs, one of the most well documented in the aquatic environment are pharmaceutical drugs, such as natural and synthetic estrogens. In particular, the widespread presence of the synthetic estrogen 17 α-Ethinylestradiol (EE2) in water may lead to bioaccumulation in sediment and biota. EE2 is the primary component in contraceptive pills, and is a derivative of the natural hormone estradiol (E2). In this study, the mussel Mytilus galloprovincialis was exposed to EE2 in a semi-static and time-dependent experiment, for a total exposure period of 28 days. Biochemical and transcriptomics analyses were performed on mussel digestive glands after exposure for 14 (T14) and 28 (T28) days. Metabolic and DNA impairments, as well as activation of antioxidant and biotransformation enzymes activation, were detected in T28 exposed mussels. RNA-Seq analysis showed significant differential expression of 160 (T14 compared to controls), 33 (T28 compared to controls) and 79 (T14 compared to T28) genes. Signs of stress after EE2 treatment included up-regulation of gene/proteins involved with immune function, lipid transport, and metabolic and antibacterial properties. This study elucidates the underlying mechanisms of EE2 in a filter feeding organisms to elucidate the effects of this human pharmaceutical on aquatic biota.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/286380
https://api.elsevier.com/content/abstract/scopus_id/85144810709
url http://hdl.handle.net/10261/286380
https://api.elsevier.com/content/abstract/scopus_id/85144810709
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Aquatic toxicology (Amsterdam, Netherlands)
https://doi.org/10.1016/j.aquatox.2022.106376
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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