Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments

Recent studies have shown that crAssphage is abundant in human faecal samples worldwide. It has thus been postulated as a potential microbial source tracking (MST) marker to detect human faecal pollution in water. However, an effective implementation of crAssphage in water management strategies will...

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Autores: Ballesté Pau, Elisenda, Pascual Benito, Miriam, Martín Díaz, Julia, Blanch i Gisbert, Anicet, Lucena Gutiérrez, Francisco, Muniesa Pérez, Ma. Teresa, Jofre i Torroella, Joan, García Aljaro, Cristina
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/195870
Acceso en línea:https://hdl.handle.net/2445/195870
Access Level:acceso abierto
Palabra clave:Bacteriòfags
Contaminació microbiana
Contaminació de l'aigua
Bacteriophages
Microbial contamination
Water pollution
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spelling Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environmentsBallesté Pau, ElisendaPascual Benito, MiriamMartín Díaz, JuliaBlanch i Gisbert, AnicetLucena Gutiérrez, FranciscoMuniesa Pérez, Ma. TeresaJofre i Torroella, JoanGarcía Aljaro, CristinaBacteriòfagsContaminació microbianaContaminació de l'aiguaBacteriophagesMicrobial contaminationWater pollutionRecent studies have shown that crAssphage is abundant in human faecal samples worldwide. It has thus been postulated as a potential microbial source tracking (MST) marker to detect human faecal pollution in water. However, an effective implementation of crAssphage in water management strategies will depend on an understanding of its environmental dynamics. In this work, the abundance and temporal distribution of crAssphage was analysed in the effluent of wastewater treatment plants using different sewage treatments, and in two rivers (water and sediments) that differ in pollution impact and flow regime. Additionally, the influence of environmental conditions (temperature and rainfall) on the removal of the marker was studied along a river section, and natural inactivation was assessed by a mesocosms approach. Molecular and culture-based tools were used to compare crAssphage abundance and dynamics with those of bacteria and bacteriophages currently applied as global indicators (E. coli, somatic coliphages, Bacteroides GA17 bacteriophages, and the human-associated MST markers HF183 and HMBif). CrAssphage concentrations in sewage effluent and river samples were similar to those of HF183 and HMBif and higher than other general and/or culture-based indicators (by 2-3 orders of magnitude). Measurement of crAssphage abundance revealed no temporal variability in the effluent, although rainfall events affected the dynamics, possibly through the mobilisation of sediments, where the marker was detected in high concentrations, and an increase in diffuse and point pollution. Another factor affecting crAssphage inactivation was temperature. Its persistence was longer compared with other bacterial markers analysed by qPCR but lower than culturable markers. The results of this study support the use of crAssphage as a human source tracking marker of faecal pollution in water, since it has similar abundances to other molecular human MST markers, yet with a longer persistence in the environment. Nevertheless, its use in combination with infectious bacteriophages is probably advisable.Elsevier Ltd2023202320192023info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion12 p.application/pdfhttps://hdl.handle.net/2445/195870Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.watres.2019.02.042Water Research, 2019, vol. 155, p. 233-244https://doi.org/10.1016/j.watres.2019.02.042cc-by-nc-nd (c) Elsevier Ltd, 2019https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1958702026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
title Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
spellingShingle Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
Ballesté Pau, Elisenda
Bacteriòfags
Contaminació microbiana
Contaminació de l'aigua
Bacteriophages
Microbial contamination
Water pollution
title_short Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
title_full Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
title_fullStr Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
title_full_unstemmed Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
title_sort Dynamics of crAssphage as a human source tracking marker in potentially faecally polluted environments
dc.creator.none.fl_str_mv Ballesté Pau, Elisenda
Pascual Benito, Miriam
Martín Díaz, Julia
Blanch i Gisbert, Anicet
Lucena Gutiérrez, Francisco
Muniesa Pérez, Ma. Teresa
Jofre i Torroella, Joan
García Aljaro, Cristina
author Ballesté Pau, Elisenda
author_facet Ballesté Pau, Elisenda
Pascual Benito, Miriam
Martín Díaz, Julia
Blanch i Gisbert, Anicet
Lucena Gutiérrez, Francisco
Muniesa Pérez, Ma. Teresa
Jofre i Torroella, Joan
García Aljaro, Cristina
author_role author
author2 Pascual Benito, Miriam
Martín Díaz, Julia
Blanch i Gisbert, Anicet
Lucena Gutiérrez, Francisco
Muniesa Pérez, Ma. Teresa
Jofre i Torroella, Joan
García Aljaro, Cristina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacteriòfags
Contaminació microbiana
Contaminació de l'aigua
Bacteriophages
Microbial contamination
Water pollution
topic Bacteriòfags
Contaminació microbiana
Contaminació de l'aigua
Bacteriophages
Microbial contamination
Water pollution
description Recent studies have shown that crAssphage is abundant in human faecal samples worldwide. It has thus been postulated as a potential microbial source tracking (MST) marker to detect human faecal pollution in water. However, an effective implementation of crAssphage in water management strategies will depend on an understanding of its environmental dynamics. In this work, the abundance and temporal distribution of crAssphage was analysed in the effluent of wastewater treatment plants using different sewage treatments, and in two rivers (water and sediments) that differ in pollution impact and flow regime. Additionally, the influence of environmental conditions (temperature and rainfall) on the removal of the marker was studied along a river section, and natural inactivation was assessed by a mesocosms approach. Molecular and culture-based tools were used to compare crAssphage abundance and dynamics with those of bacteria and bacteriophages currently applied as global indicators (E. coli, somatic coliphages, Bacteroides GA17 bacteriophages, and the human-associated MST markers HF183 and HMBif). CrAssphage concentrations in sewage effluent and river samples were similar to those of HF183 and HMBif and higher than other general and/or culture-based indicators (by 2-3 orders of magnitude). Measurement of crAssphage abundance revealed no temporal variability in the effluent, although rainfall events affected the dynamics, possibly through the mobilisation of sediments, where the marker was detected in high concentrations, and an increase in diffuse and point pollution. Another factor affecting crAssphage inactivation was temperature. Its persistence was longer compared with other bacterial markers analysed by qPCR but lower than culturable markers. The results of this study support the use of crAssphage as a human source tracking marker of faecal pollution in water, since it has similar abundances to other molecular human MST markers, yet with a longer persistence in the environment. Nevertheless, its use in combination with infectious bacteriophages is probably advisable.
publishDate 2019
dc.date.none.fl_str_mv 2019
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/195870
url https://hdl.handle.net/2445/195870
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.watres.2019.02.042
Water Research, 2019, vol. 155, p. 233-244
https://doi.org/10.1016/j.watres.2019.02.042
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier Ltd, 2019
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier Ltd, 2019
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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