Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species

9 páginas

Detalles Bibliográficos
Autores: Martínez, Iciar, Velasco, Amaya, Pérez Martín, Ricardo Isaac, Foras, Eskil, Yamashita, Michiaki, González Sotelo, Carmen
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/65148
Acceso en línea:http://hdl.handle.net/10261/65148
Access Level:acceso abierto
Palabra clave:Species identification
Fish
Mackerel
Scomber
PCR
FINS
mt DNA
RFLP-PCR,
Saba
Caballa
Scombrus
Colias
Japonicus
Australasicus
Traceability
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spelling Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber speciesMartínez, IciarVelasco, AmayaPérez Martín, Ricardo IsaacForas, EskilYamashita, MichiakiGonzález Sotelo, CarmenSpecies identificationFishMackerelScomberPCRFINSmt DNARFLP-PCR,SabaCaballaScombrusColiasJaponicusAustralasicusTraceability9 páginasThe purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicusPeer reviewedTaylor & Francis2012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/65148reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1080/10498850.2012.664804info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/651482026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
title Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
spellingShingle Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
Martínez, Iciar
Species identification
Fish
Mackerel
Scomber
PCR
FINS
mt DNA
RFLP-PCR,
Saba
Caballa
Scombrus
Colias
Japonicus
Australasicus
Traceability
title_short Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
title_full Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
title_fullStr Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
title_full_unstemmed Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
title_sort Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species
dc.creator.none.fl_str_mv Martínez, Iciar
Velasco, Amaya
Pérez Martín, Ricardo Isaac
Foras, Eskil
Yamashita, Michiaki
González Sotelo, Carmen
author Martínez, Iciar
author_facet Martínez, Iciar
Velasco, Amaya
Pérez Martín, Ricardo Isaac
Foras, Eskil
Yamashita, Michiaki
González Sotelo, Carmen
author_role author
author2 Velasco, Amaya
Pérez Martín, Ricardo Isaac
Foras, Eskil
Yamashita, Michiaki
González Sotelo, Carmen
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Species identification
Fish
Mackerel
Scomber
PCR
FINS
mt DNA
RFLP-PCR,
Saba
Caballa
Scombrus
Colias
Japonicus
Australasicus
Traceability
topic Species identification
Fish
Mackerel
Scomber
PCR
FINS
mt DNA
RFLP-PCR,
Saba
Caballa
Scombrus
Colias
Japonicus
Australasicus
Traceability
description 9 páginas
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/65148
url http://hdl.handle.net/10261/65148
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1080/10498850.2012.664804
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418221420937216
score 15.811543

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