Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells

[Objective]: The lack of success with classical targeted therapies in cancer treatment has forced researchers to employ either combined therapies or agents that interfere with multiple pathways. In this study, a proteomic evaluation was performed to understand the in vitro antiproliferative effect o...

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Autores: Çelebier, Mustafa, Simó, Carolina, Altınöz, Sacide, Cifuentes, Alejandro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/149802
Acceso en línea:http://hdl.handle.net/10261/149802
Access Level:acceso abierto
Palabra clave:MALDI-TOF
Rosemary polyphenols
Leukemia
Foodomics
Proteomics
2-DE Electrophoresis
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spelling Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cellsGıda kaynaklı polifenollerin K562 lösemi hücreleri üzerine in vitro çoğalmayı önleyici etkisinin karşılaştırmalı proteomik çalışmalarla incelenmesiÇelebier, MustafaSimó, CarolinaAltınöz, SacideCifuentes, AlejandroMALDI-TOFRosemary polyphenolsLeukemiaFoodomicsProteomics2-DE Electrophoresis[Objective]: The lack of success with classical targeted therapies in cancer treatment has forced researchers to employ either combined therapies or agents that interfere with multiple pathways. In this study, a proteomic evaluation was performed to understand the in vitro antiproliferative effect of dietary compounds against leukemia cells in proteome level. Typical human cell models of leukemia (i.e., wild K562 cells and multi-drug resistant leukemia cells K562/R) were treated with rosemary (Rosmarinus officinalis L.) extracts rich in polyphenols and a comparative proteomic study was performed to identify up- or down-regulated proteins by in vitro antiproliferative effect of polyphenols. [Methods]: The protein fraction from the polyphenol-treated and control K562 and K562/R cells were separated through two dimensional polyacrylamide gel electrophoresis (2DE) and their proteomic profiles compared by image analysis. The proteins identified to be overexpressed or underexpressed in K562 and K562/R cells after the polyphenol treatment were identified by MALDI-TOF/TOF MS. [Results]: The results in this study were evaluated to understand the mode of action of these dietary constituents against leukemia cell proliferation. Dietary polyphenols extracted from rosemary bring about the up regulation or down regulation of different proteins on leukemia cells. These proteins are related to tumorigenesis, cancer proliferation and antioxidant activity. [Conclusion]: Our results revealed that the studied rosemary extract rich in polyphenols induced the down regulation of adenine phosphoribosyl transferase and annexin A1 in K562/R cell lines and tubulin alpha-1C chain in K562 cell lines. According to the expression proteomics results in this study, it could be concluded that the rosemary polyphenols shows in vitro antiproliferative activity in K562 and K562/R leukemia cell lines.Mustafa Çelebier thanks to The Council of Higher Education, Turkey for his grant. This work was supported by AGL2008-05108-C03-01 (Ministerio de Ciencia e Innovación, Spain).Peer ReviewedWalter de GruyterMinisterio de Ciencia e Innovación (España)Council of Higher Education (Türkiye)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/149802reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.5505/tjb.2015.29484Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1498022026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
Gıda kaynaklı polifenollerin K562 lösemi hücreleri üzerine in vitro çoğalmayı önleyici etkisinin karşılaştırmalı proteomik çalışmalarla incelenmesi
title Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
spellingShingle Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
Çelebier, Mustafa
MALDI-TOF
Rosemary polyphenols
Leukemia
Foodomics
Proteomics
2-DE Electrophoresis
title_short Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
title_full Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
title_fullStr Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
title_full_unstemmed Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
title_sort Comparative proteomics to investigate the in vitro antiproliferative effect of dietary polyphenols against K562 leukemia cells
dc.creator.none.fl_str_mv Çelebier, Mustafa
Simó, Carolina
Altınöz, Sacide
Cifuentes, Alejandro
author Çelebier, Mustafa
author_facet Çelebier, Mustafa
Simó, Carolina
Altınöz, Sacide
Cifuentes, Alejandro
author_role author
author2 Simó, Carolina
Altınöz, Sacide
Cifuentes, Alejandro
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Council of Higher Education (Türkiye)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv MALDI-TOF
Rosemary polyphenols
Leukemia
Foodomics
Proteomics
2-DE Electrophoresis
topic MALDI-TOF
Rosemary polyphenols
Leukemia
Foodomics
Proteomics
2-DE Electrophoresis
description [Objective]: The lack of success with classical targeted therapies in cancer treatment has forced researchers to employ either combined therapies or agents that interfere with multiple pathways. In this study, a proteomic evaluation was performed to understand the in vitro antiproliferative effect of dietary compounds against leukemia cells in proteome level. Typical human cell models of leukemia (i.e., wild K562 cells and multi-drug resistant leukemia cells K562/R) were treated with rosemary (Rosmarinus officinalis L.) extracts rich in polyphenols and a comparative proteomic study was performed to identify up- or down-regulated proteins by in vitro antiproliferative effect of polyphenols. [Methods]: The protein fraction from the polyphenol-treated and control K562 and K562/R cells were separated through two dimensional polyacrylamide gel electrophoresis (2DE) and their proteomic profiles compared by image analysis. The proteins identified to be overexpressed or underexpressed in K562 and K562/R cells after the polyphenol treatment were identified by MALDI-TOF/TOF MS. [Results]: The results in this study were evaluated to understand the mode of action of these dietary constituents against leukemia cell proliferation. Dietary polyphenols extracted from rosemary bring about the up regulation or down regulation of different proteins on leukemia cells. These proteins are related to tumorigenesis, cancer proliferation and antioxidant activity. [Conclusion]: Our results revealed that the studied rosemary extract rich in polyphenols induced the down regulation of adenine phosphoribosyl transferase and annexin A1 in K562/R cell lines and tubulin alpha-1C chain in K562 cell lines. According to the expression proteomics results in this study, it could be concluded that the rosemary polyphenols shows in vitro antiproliferative activity in K562 and K562/R leukemia cell lines.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/149802
url http://hdl.handle.net/10261/149802
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.5505/tjb.2015.29484
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Walter de Gruyter
publisher.none.fl_str_mv Walter de Gruyter
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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