Molecular Detection of Lymph Node Metastases with One-Step Nucleic Acid Amplification (OSNA) Pooling in Prostate Cancer

Pelvic lymph node dissection (PLND) is the most accurate procedure for lymph node (LN) staging in prostate cancer (PCa) patients. LN sectioning and hematoxylin and eosin (H&E) staining of at least one slice remains the gold standard for LN evaluation, potentially leading to misdetection of small...

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Autores: Cuadras, Mercè|||0000-0003-4715-9230, Semidey Raven, Maria Eugenia|||0000-0001-8539-9265, Planas Morin, Jacques|||0000-0002-0222-584X, de Torres, Inés|||0000-0002-5495-9140, Regis, Lucas|||0000-0001-7121-1946, Celma, Ana|||0000-0002-3128-3811, Trilla Herrera, Enrique|||0000-0001-9401-0872, Ramón y Cajal, Santiago|||0000-0002-3867-1390, Medina, Rafael A.|||0000-0002-4514-4329, Congregado, Belén, Marcilla, David, Japón, Miguel A.|||0000-0002-5980-5044, Ramirez, Miguel|||0000-0001-6214-784X, Calatrava-Fons, Ana, Leivar, Asier, Alonso, María B., García, Eugenia, González-Peramato, Pilar, Vazquez-Martul, Dario|||0000-0003-3060-1897, Concha-López, Ángel, Chantada, Venancio, Queipo, Francisco J.|||0000-0002-4173-6300, Gago Ramos, José Luis, Carrato, Cristina|||0000-0002-1953-8287, Luque, Rafael J.|||0000-0002-9808-784X, Moreno-Jimenez, Juan, Catalina-Fernández, Inmaculada, León, Cristina, Morote Robles, Juan|||0000-0002-2168-323X
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:307845
Acceso en línea:https://ddd.uab.cat/record/307845
https://dx.doi.org/urn:doi:10.3390/ijms252413489
Access Level:acceso abierto
Palabra clave:Prostate cancer
Pelvic lymph node dissection
Lymph node metastases
OSNA
Cytokeratin 19
Pooling
Hematoxylin and eosin staining
Descripción
Sumario:Pelvic lymph node dissection (PLND) is the most accurate procedure for lymph node (LN) staging in prostate cancer (PCa) patients. LN sectioning and hematoxylin and eosin (H&E) staining of at least one slice remains the gold standard for LN evaluation, potentially leading to misdetection of small metastatic focus. Entire LN analysis is possible with One-Step Nucleic Acid Amplification (OSNA) by detecting cytokeratin 19 (CK19) mRNA as a surrogate for LN invasion. This study aimed to compare postoperative performance of OSNA pooling with conventional H&E staining for pathological LN detection in PCa patients. POPCORN was an observational, prospective, and multicenter study of patients with PCa who underwent PLND. Dissected LNs were analyzed by both methods. This study included 2503 LNs from 131 patients, showing no statistically significant differences in pathological LN detection. Concordance between methods was high (93.9%), as were specificity (96.6%) and negative predictive value (96.6%) of OSNA pooling. The measure of agreement (Cohen's Kappa [κ]) was 0.70. Only eight (6.1%) discordances were observed, including four misdetections from each method. Results showed a high concordance between OSNA pooling and H&E staining, suggesting that OSNA pooling may be a good alternative to H&E staining to detect LN metastases in PCa patients.

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