Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production

20 p.-6 fig.-6 tab.

Detalles Bibliográficos
Autores: Azzouz, Zahra, Bettache, Azzeddine, Boucherba, Nawel, Prieto Orzanco, Alicia, Martínez, María Jesús, Benallaoua, Said, Eugenio, Laura I. de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/263314
Acceso en línea:http://hdl.handle.net/10261/263314
Access Level:acceso abierto
Palabra clave:Fungi
Enzymes
Hemicellulose
Xylan
Wastes
Hydrolysis
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spelling Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides productionAzzouz, ZahraBettache, AzzeddineBoucherba, NawelPrieto Orzanco, AliciaMartínez, María JesúsBenallaoua, SaidEugenio, Laura I. deFungiEnzymesHemicelluloseXylanWastesHydrolysis20 p.-6 fig.-6 tab.Plant biomass constitutes the main source of renewable carbon on the planet. Its valorization has traditionally been focused on the use of cellulose, although hemicellulose is the second most abundant group of polysaccharides on Earth. The main enzymes involved in plant biomass degradation are glycosyl hydrolases, and filamentous fungi are good producers of these enzymes. In this study, a new strain of Aspergillus niger was used for hemicellulase production under solid-state fermentation using wheat straw as single-carbon source. Physicochemical parameters for the production of an endoxylanase were optimized by using a One-Factor-at-a-Time (OFAT) approach and response surface methodology (RSM). Maximum xylanase yield after RSM optimization was increased 3-fold, and 1.41- fold purification was achieved after ultrafiltration and ion-exchange chromatography, with about 6.2% yield. The highest activity of the purified xylanase was observed at 50 °C and pH 6. The enzyme displayed high thermal and pH stability, with more than 90% residual activity between pH 3.0–9.0 and between 30–40 °C, after 24 h of incubation, with half-lives of 30 min at 50 and 60 °C. The enzyme was mostly active against wheat arabinoxylan, and its kinetic parameters were analyzed (Km = 26.06 mg·mL−1 and Vmax = 5.647 U·mg−1). Wheat straw xylan hydrolysis with the purified β-1,4 endoxylanase showed that it was able to release xylooligosaccharides, making it suitable for different applications in food technology.This work has been funded by Projects GLYSUS RTI2018-093683-Β-I00 (MCIU/AEI/FEDER) and RETOPROSOST2 S2018/EMT-4459 (Comunidad de Madrid).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Comunidad de MadridAzzouz, Zahra [0000-0001-5524-238X]Bettache, Azzeddine [0000-0003-4813-1387]Boucherba, Nawel [0000-0002-7273-1019]Prieto, Alicia [0000-0002-5075-4025]Martínez, María Jesús [0000-0003-2166-1097]Benallaoua, Said [0000-0002-3095-8792]Eugenio, Laura I. de [0000-0002-0496-8663]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/263314reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093683-Β-I00S2018/EMT-4459/RETOPROSOST2https://doi.org/10.3390/molecules26092527Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2633142026-05-22T06:33:51Z
dc.title.none.fl_str_mv Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
title Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
spellingShingle Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
Azzouz, Zahra
Fungi
Enzymes
Hemicellulose
Xylan
Wastes
Hydrolysis
title_short Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
title_full Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
title_fullStr Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
title_full_unstemmed Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
title_sort Optimization of β-1,4-endoxylanase production by an Aspergillus niger strain growing on wheat straw and application in xylooligosaccharides production
dc.creator.none.fl_str_mv Azzouz, Zahra
Bettache, Azzeddine
Boucherba, Nawel
Prieto Orzanco, Alicia
Martínez, María Jesús
Benallaoua, Said
Eugenio, Laura I. de
author Azzouz, Zahra
author_facet Azzouz, Zahra
Bettache, Azzeddine
Boucherba, Nawel
Prieto Orzanco, Alicia
Martínez, María Jesús
Benallaoua, Said
Eugenio, Laura I. de
author_role author
author2 Bettache, Azzeddine
Boucherba, Nawel
Prieto Orzanco, Alicia
Martínez, María Jesús
Benallaoua, Said
Eugenio, Laura I. de
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Comunidad de Madrid
Azzouz, Zahra [0000-0001-5524-238X]
Bettache, Azzeddine [0000-0003-4813-1387]
Boucherba, Nawel [0000-0002-7273-1019]
Prieto, Alicia [0000-0002-5075-4025]
Martínez, María Jesús [0000-0003-2166-1097]
Benallaoua, Said [0000-0002-3095-8792]
Eugenio, Laura I. de [0000-0002-0496-8663]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fungi
Enzymes
Hemicellulose
Xylan
Wastes
Hydrolysis
topic Fungi
Enzymes
Hemicellulose
Xylan
Wastes
Hydrolysis
description 20 p.-6 fig.-6 tab.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/263314
url http://hdl.handle.net/10261/263314
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093683-Β-I00
S2018/EMT-4459/RETOPROSOST2
https://doi.org/10.3390/molecules26092527
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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