The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions

Elucidating the cellular and molecular mechanisms that regulate the balance between progenitor cell proliferation and neuronal differentiation in the construction of the embryonic brain demands the combination of cell lineage and functional approaches. Here, we generate the comprehensive lineage of...

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Detalles Bibliográficos
Autores: Hevia, Covadonga F., Engel-Pizcueta, Carolyn, Udina, Frederic, Pujades Corbi, Cristina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/54000
Acceso en línea:http://hdl.handle.net/10230/54000
http://dx.doi.org/10.1016/j.celrep.2022.110915
Access Level:acceso abierto
Palabra clave:CP: Developmental biology
CP: Neuroscience
Notch signaling
Boundaries
Cell fate
Cell lineage
Hindbrain
Morphogenesis
Neural progenitors
Neurogenesis
Neuronal differentiation
Radial glia
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spelling The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisionsHevia, Covadonga F.Engel-Pizcueta, CarolynUdina, FredericPujades Corbi, CristinaCP: Developmental biologyCP: NeuroscienceNotch signalingBoundariesCell fateCell lineageHindbrainMorphogenesisNeural progenitorsNeurogenesisNeuronal differentiationRadial gliaElucidating the cellular and molecular mechanisms that regulate the balance between progenitor cell proliferation and neuronal differentiation in the construction of the embryonic brain demands the combination of cell lineage and functional approaches. Here, we generate the comprehensive lineage of hindbrain boundary cells by using a CRISPR-based knockin zebrafish transgenic line that specifically labels the boundaries. We unveil that boundary cells asynchronously engage in neurogenesis undergoing a functional transition from neuroepithelial progenitors to radial glia cells, coinciding with the onset of Notch3 signaling that triggers their asymmetrical cell division. Upon notch3 loss of function, boundary cells lose radial glia properties and symmetrically divide undergoing neuronal differentiation. Finally, we show that the fate of boundary cells is to become neurons, the subtype of which relies on their axial position, suggesting that boundary cells contribute to refine the number and proportion of the distinct neuronal populations.This work was funded by grant PGC2018-095663-B-I00 from Spanish Ministry of Science and Innovation (MICIN), Agencia Estatal de Investigación (AEI), and Fondo Europeo de Desarrollo Regional (FEDER) to C.P. The Department of Medicine and Life Sciences (UPF) is an Unidad de Excelencia María de Maeztu funded by the MICIN and the AEI (DOI: 10.13039/501100011033) Ref: CEX2018-000792-M. C.E.P. is a recipient of a predoctoral FPU fellowship from the Spanish Ministry of Universities. F.U.’s work was supported by PGC2018-101643-B-I00 (MICIN/AEI-FEDER). C.P. is a recipient of ICREA Academia award (Generalitat de Catalunya).Elsevier202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/54000http://dx.doi.org/10.1016/j.celrep.2022.110915reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésCell Rep. 2022 Jun 7;39(10):110915info:eu-repo/grantAgreement/ES/2PE/PGC2018-095663-B-I00info:eu-repo/grantAgreement/ES/2PE/PGC2018-101643-B-I00© 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/540002026-05-29T05:05:01Z
dc.title.none.fl_str_mv The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
title The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
spellingShingle The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
Hevia, Covadonga F.
CP: Developmental biology
CP: Neuroscience
Notch signaling
Boundaries
Cell fate
Cell lineage
Hindbrain
Morphogenesis
Neural progenitors
Neurogenesis
Neuronal differentiation
Radial glia
title_short The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
title_full The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
title_fullStr The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
title_full_unstemmed The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
title_sort The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions
dc.creator.none.fl_str_mv Hevia, Covadonga F.
Engel-Pizcueta, Carolyn
Udina, Frederic
Pujades Corbi, Cristina
author Hevia, Covadonga F.
author_facet Hevia, Covadonga F.
Engel-Pizcueta, Carolyn
Udina, Frederic
Pujades Corbi, Cristina
author_role author
author2 Engel-Pizcueta, Carolyn
Udina, Frederic
Pujades Corbi, Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv CP: Developmental biology
CP: Neuroscience
Notch signaling
Boundaries
Cell fate
Cell lineage
Hindbrain
Morphogenesis
Neural progenitors
Neurogenesis
Neuronal differentiation
Radial glia
topic CP: Developmental biology
CP: Neuroscience
Notch signaling
Boundaries
Cell fate
Cell lineage
Hindbrain
Morphogenesis
Neural progenitors
Neurogenesis
Neuronal differentiation
Radial glia
description Elucidating the cellular and molecular mechanisms that regulate the balance between progenitor cell proliferation and neuronal differentiation in the construction of the embryonic brain demands the combination of cell lineage and functional approaches. Here, we generate the comprehensive lineage of hindbrain boundary cells by using a CRISPR-based knockin zebrafish transgenic line that specifically labels the boundaries. We unveil that boundary cells asynchronously engage in neurogenesis undergoing a functional transition from neuroepithelial progenitors to radial glia cells, coinciding with the onset of Notch3 signaling that triggers their asymmetrical cell division. Upon notch3 loss of function, boundary cells lose radial glia properties and symmetrically divide undergoing neuronal differentiation. Finally, we show that the fate of boundary cells is to become neurons, the subtype of which relies on their axial position, suggesting that boundary cells contribute to refine the number and proportion of the distinct neuronal populations.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/54000
http://dx.doi.org/10.1016/j.celrep.2022.110915
url http://hdl.handle.net/10230/54000
http://dx.doi.org/10.1016/j.celrep.2022.110915
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cell Rep. 2022 Jun 7;39(10):110915
info:eu-repo/grantAgreement/ES/2PE/PGC2018-095663-B-I00
info:eu-repo/grantAgreement/ES/2PE/PGC2018-101643-B-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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