Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.

Gender difference in Parkinson’s disease (PD) suggests that female sex steroids may promote dopaminergic neuron survival and protect them from degeneration. The glial cell line-derived neurotrophic factor (GDNF) is believed to be dopaminotrophic; thus it is considered as a potential therapeutic targ...

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Autores: Enterría Morales, Daniel, López López, Ivette, López Barneo, José, D’Anglemont de Tassigny, Xavier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/96037
Acceso en línea:https://hdl.handle.net/11441/96037
https://doi.org/10.1371/journal.pone.0164391
Access Level:acceso abierto
Palabra clave:Striatal GDNF Production
Parkinson’s disease
MRNA and protein synthesis
Female Mouse
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spelling Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.Enterría Morales, DanielLópez López, IvetteLópez Barneo, JoséD’Anglemont de Tassigny, XavierStriatal GDNF ProductionParkinson’s diseaseMRNA and protein synthesisFemale MouseGender difference in Parkinson’s disease (PD) suggests that female sex steroids may promote dopaminergic neuron survival and protect them from degeneration. The glial cell line-derived neurotrophic factor (GDNF) is believed to be dopaminotrophic; thus it is considered as a potential therapeutic target in PD. Additionally, GDNF is endogenously synthetized in the caudate/putamen of humans and striatum in rodents. A neuroprotective role of estrogens on the nigrostriatal pathway via the stimulation of GDNF has been proposed. Since the GDNF-producing parvalbumin (Parv) interneurons express the estrogen receptor alpha in the mouse striatum, we sought to determine whether ectopic estrogenic compound mod- ulates the GDNF synthesis in mice. Using an ovariectomized-estradiol (E 2 ) replacement regimen, which reliably generates a rise of plasma estradiol, we assessed the effects of dif- ferent levels of E 2 on the activation of striatal neuronal populations, and GDNF production. A strong correlation was found between plasma E 2 and the expression of the immediate early gene cFos in the striatum, as well as in other cortical regions. However, moderate and high E 2 treatments failed to induce any striatal GDNF mRNA and protein synthesis. High E 2 only stimulates cFos induction in a low percentage of striatal Parv neurons whereas the majority of cFos-positive cells are medium spiny neurons. Activation of these projecting neurons by E 2 suggests a role of circulating sex steroids in the modulation of striatal neural pathways.Instituto de Salud Carlos III, Miguel Servet Asociación CP12- 03217 (XDADT)Ministerio de Economı́a y Competitividad: Plan Estatal de Investigación Cientı́fica y Técnica y de Innovación 2013-2016 (JLB)Fisiología Médica y BiofísicaCTS516: Fisiologia celular y biofisica2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/96037https://doi.org/10.1371/journal.pone.0164391reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPlos One, 11 (10)info:eu-repo/semantics/openAccessoai:idus.us.es:11441/960372026-06-17T12:51:07Z
dc.title.none.fl_str_mv Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
title Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
spellingShingle Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
Enterría Morales, Daniel
Striatal GDNF Production
Parkinson’s disease
MRNA and protein synthesis
Female Mouse
title_short Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
title_full Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
title_fullStr Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
title_full_unstemmed Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
title_sort Striatal GDNF Production Is Independent to Circulating Estradiol Level Despite Pan- Neuronal Activation in the Female Mouse.
dc.creator.none.fl_str_mv Enterría Morales, Daniel
López López, Ivette
López Barneo, José
D’Anglemont de Tassigny, Xavier
author Enterría Morales, Daniel
author_facet Enterría Morales, Daniel
López López, Ivette
López Barneo, José
D’Anglemont de Tassigny, Xavier
author_role author
author2 López López, Ivette
López Barneo, José
D’Anglemont de Tassigny, Xavier
author2_role author
author
author
dc.contributor.none.fl_str_mv Fisiología Médica y Biofísica
CTS516: Fisiologia celular y biofisica
dc.subject.none.fl_str_mv Striatal GDNF Production
Parkinson’s disease
MRNA and protein synthesis
Female Mouse
topic Striatal GDNF Production
Parkinson’s disease
MRNA and protein synthesis
Female Mouse
description Gender difference in Parkinson’s disease (PD) suggests that female sex steroids may promote dopaminergic neuron survival and protect them from degeneration. The glial cell line-derived neurotrophic factor (GDNF) is believed to be dopaminotrophic; thus it is considered as a potential therapeutic target in PD. Additionally, GDNF is endogenously synthetized in the caudate/putamen of humans and striatum in rodents. A neuroprotective role of estrogens on the nigrostriatal pathway via the stimulation of GDNF has been proposed. Since the GDNF-producing parvalbumin (Parv) interneurons express the estrogen receptor alpha in the mouse striatum, we sought to determine whether ectopic estrogenic compound mod- ulates the GDNF synthesis in mice. Using an ovariectomized-estradiol (E 2 ) replacement regimen, which reliably generates a rise of plasma estradiol, we assessed the effects of dif- ferent levels of E 2 on the activation of striatal neuronal populations, and GDNF production. A strong correlation was found between plasma E 2 and the expression of the immediate early gene cFos in the striatum, as well as in other cortical regions. However, moderate and high E 2 treatments failed to induce any striatal GDNF mRNA and protein synthesis. High E 2 only stimulates cFos induction in a low percentage of striatal Parv neurons whereas the majority of cFos-positive cells are medium spiny neurons. Activation of these projecting neurons by E 2 suggests a role of circulating sex steroids in the modulation of striatal neural pathways.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/96037
https://doi.org/10.1371/journal.pone.0164391
url https://hdl.handle.net/11441/96037
https://doi.org/10.1371/journal.pone.0164391
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plos One, 11 (10)
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
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