Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours

miR-17-5p and E2F1 have been described as deregulated in cancer, but they have scarcely been studied in pituitary neuroendocrine tumours (PitNETs). This study evaluates the relationship of E2F1 and miR-17-5p with the invasiveness and proliferation of PitNETs. In this cross-sectional descriptive stud...

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Authors: García Martínez, Araceli, López Muñoz, Beatriz, Fajardo Montañana, Carmen, Cámara, Rosa, Lamas Oliveira, Cristina, Silva Ortega, Sandra, Aranda, Ignacio, Picó, Antonio
Format: article
Publication Date:2020
Country:España
Institution:Universidad de Castilla-La Mancha
Repository:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/42660
Online Access:https://www.mdpi.com/2075-4418/10/4/227
https://hdl.handle.net/10578/42660
Access Level:Open access
Keyword:E2F1
MicroRNAs
miR-17~92
Pituitary neuroendocrine tumours
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spelling Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine TumoursGarcía Martínez, AraceliLópez Muñoz, BeatrizFajardo Montañana, CarmenCámara, RosaLamas Oliveira, CristinaSilva Ortega, SandraAranda, IgnacioPicó, AntonioE2F1MicroRNAsmiR-17~92Pituitary neuroendocrine tumoursmiR-17-5p and E2F1 have been described as deregulated in cancer, but they have scarcely been studied in pituitary neuroendocrine tumours (PitNETs). This study evaluates the relationship of E2F1 and miR-17-5p with the invasiveness and proliferation of PitNETs. In this cross-sectional descriptive study, we evaluated the expression of E2F1, MYC, and miR-17-5p by quantitative real time PCR analysis in 60 PitNETs: 29 gonadotroph (GT), 15 functioning somatotroph (ST), and 16 corticotroph (CT) tumours, of which 8 were silent (sCT). The clinical data were collected from the Spanish Molecular Register of Pituitary Adenomas (REMAH) database. We defined invasiveness according to the Knosp classification and proliferation according to a molecular expression of Ki-67 = 2.59. E2F1 was more expressed in invasive than in non-invasive tumours in the whole series (p = 0.004) and in STs (p = 0.01). In addition, it was overexpressed in the silent subtypes (GTs and sCTs; all macroadenomas) and normoexpressed in the functioning ones (fCTs and STs; some microadenomas). miR-17-5p was more expressed in proliferative than in non-proliferative tumours (p = 0.041) in the whole series but not by subtypes. Conclusions: Our study suggests that in PitNETs, E2F1 could be a good biomarker of invasiveness, and miR-17-5p of proliferation, helping the clinical management of these tumours.MDPI202520252020info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://www.mdpi.com/2075-4418/10/4/227https://hdl.handle.net/10578/42660reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésInvestigator Initiated Research (IIR) Program from Pfizer (WS1568384)info:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/426602026-05-27T07:36:41Z
dc.title.none.fl_str_mv Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
title Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
spellingShingle Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
García Martínez, Araceli
E2F1
MicroRNAs
miR-17~92
Pituitary neuroendocrine tumours
title_short Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
title_full Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
title_fullStr Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
title_full_unstemmed Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
title_sort Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
dc.creator.none.fl_str_mv García Martínez, Araceli
López Muñoz, Beatriz
Fajardo Montañana, Carmen
Cámara, Rosa
Lamas Oliveira, Cristina
Silva Ortega, Sandra
Aranda, Ignacio
Picó, Antonio
author García Martínez, Araceli
author_facet García Martínez, Araceli
López Muñoz, Beatriz
Fajardo Montañana, Carmen
Cámara, Rosa
Lamas Oliveira, Cristina
Silva Ortega, Sandra
Aranda, Ignacio
Picó, Antonio
author_role author
author2 López Muñoz, Beatriz
Fajardo Montañana, Carmen
Cámara, Rosa
Lamas Oliveira, Cristina
Silva Ortega, Sandra
Aranda, Ignacio
Picó, Antonio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv E2F1
MicroRNAs
miR-17~92
Pituitary neuroendocrine tumours
topic E2F1
MicroRNAs
miR-17~92
Pituitary neuroendocrine tumours
description miR-17-5p and E2F1 have been described as deregulated in cancer, but they have scarcely been studied in pituitary neuroendocrine tumours (PitNETs). This study evaluates the relationship of E2F1 and miR-17-5p with the invasiveness and proliferation of PitNETs. In this cross-sectional descriptive study, we evaluated the expression of E2F1, MYC, and miR-17-5p by quantitative real time PCR analysis in 60 PitNETs: 29 gonadotroph (GT), 15 functioning somatotroph (ST), and 16 corticotroph (CT) tumours, of which 8 were silent (sCT). The clinical data were collected from the Spanish Molecular Register of Pituitary Adenomas (REMAH) database. We defined invasiveness according to the Knosp classification and proliferation according to a molecular expression of Ki-67 = 2.59. E2F1 was more expressed in invasive than in non-invasive tumours in the whole series (p = 0.004) and in STs (p = 0.01). In addition, it was overexpressed in the silent subtypes (GTs and sCTs; all macroadenomas) and normoexpressed in the functioning ones (fCTs and STs; some microadenomas). miR-17-5p was more expressed in proliferative than in non-proliferative tumours (p = 0.041) in the whole series but not by subtypes. Conclusions: Our study suggests that in PitNETs, E2F1 could be a good biomarker of invasiveness, and miR-17-5p of proliferation, helping the clinical management of these tumours.
publishDate 2020
dc.date.none.fl_str_mv 2020
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://www.mdpi.com/2075-4418/10/4/227
https://hdl.handle.net/10578/42660
url https://www.mdpi.com/2075-4418/10/4/227
https://hdl.handle.net/10578/42660
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Investigator Initiated Research (IIR) Program from Pfizer (WS1568384)
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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