Fluorinated Man9 as a high mannose mimetic to unravel its recogni-tion by DC-SIGN using NMR
Lectins are capable of reading out the structural information contained in carbohydrates through specific recognition processes. Determining the binding epitope of the sugar is fundamental to understanding this recognition event. NMR is a powerful tool to obtain this structural information in soluti...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/343284 |
| Acceso en línea: | http://hdl.handle.net/10261/343284 |
| Access Level: | acceso abierto |
| Palabra clave: | Carbohydrate-lectin interaction DC-SIGN Fluor-mannose High mannose NMR |
| Sumario: | Lectins are capable of reading out the structural information contained in carbohydrates through specific recognition processes. Determining the binding epitope of the sugar is fundamental to understanding this recognition event. NMR is a powerful tool to obtain this structural information in solution; however, when the sugar involved is a complex oligosaccharide such as high-mannose, the signal overlap found in the NMR spectra precludes an accurate analysis of the interaction. The introduction of tags into these complex oligosaccharides could overcome these problems and facilitate the NMR studies. Here, we show the preparation of the Man9 of high-mannose with some fluorine tags and the study of the interaction with its receptor, DC-SIGN. This fluorinated ligand has allowed us to apply heteronuclear 2D 1H,19F STD-TOCSYreF NMR experiments, using the initial slope approach, which has facilitated the analysis of the Man9/DC-SIGN interaction unequivocally providing the binding epitope. |
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