Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins

The identity and function of a given cell type relies on the differential expression of gene batteries that promote diverse phenotypes and functional specificities. Therefore, the identification of the molecular and morphological fingerprints of cell types across taxa is essential for untangling the...

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Autores: Paganos, Periklis, Ronchi, Paolo, Carl, Jil, Mizzon, Giulia, Martínez Serra, Pedro, Benvenuto, Giovanna, Arnone, Maria Ina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/192423
Acceso en línea:https://hdl.handle.net/2445/192423
Access Level:acceso abierto
Palabra clave:Genètica molecular
Morfogènesi
Regulació genètica
Pàncrees
Molecular genetics
Morphogenesis
Genetic regulation
Pancreas
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spelling Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchinsPaganos, PeriklisRonchi, PaoloCarl, JilMizzon, GiuliaMartínez Serra, PedroBenvenuto, GiovannaArnone, Maria InaGenètica molecularMorfogènesiRegulació genèticaPàncreesMolecular geneticsMorphogenesisGenetic regulationPancreasThe identity and function of a given cell type relies on the differential expression of gene batteries that promote diverse phenotypes and functional specificities. Therefore, the identification of the molecular and morphological fingerprints of cell types across taxa is essential for untangling their evolution. Here we use a multidisciplinary approach to identify the molecular and morphological features of an exocrine, pancreas-like cell type harbored within the sea urchin larval gut. Using single cell transcriptomics, we identify various cell populations with a pancreatic-like molecular fingerprint that are enriched within the S. purpuratus larva digestive tract. Among these, in the region where they reside, the midgut/stomach domain, we find that populations of exocrine pancreas-like cells have a unique regulatory wiring distinct from the rest the of the cell types of the same region. Furthermore, Serial Block-face scanning Electron Microscopy (SBEM) of the exocrine cells shows that this reported molecular diversity is associated to distinct morphological features that reflect the physiological and functional properties of this cell type. Therefore, we propose that these sea urchin exocrine cells are homologous to the well-known mammalian pancreatic acinar cells and thus we trace the origin of this particular cell type to the time of deuterostome diversification. Overall, our approach allows a thorough characterization of a complex cell type and shows how both the transcriptomic and morphological information contribute to disentangling the evolution of cell types and organs such as the pancreatic cells and pancreas. Keywords: SBEM; acinar cells; evolution of cell types; morphology; pancreas; scRNAseq; sea urchin.Frontiers Media2023202320222023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/192423Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fcell.2022.991664Frontiers In Cell And Developmental Biology, 2022, vol. 10:991664https://doi.org/10.3389/fcell.2022.991664cc-by (c) Paganos, Periklis et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1924232026-05-29T05:05:01Z
dc.title.none.fl_str_mv Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
title Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
spellingShingle Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
Paganos, Periklis
Genètica molecular
Morfogènesi
Regulació genètica
Pàncrees
Molecular genetics
Morphogenesis
Genetic regulation
Pancreas
title_short Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
title_full Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
title_fullStr Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
title_full_unstemmed Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
title_sort Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
dc.creator.none.fl_str_mv Paganos, Periklis
Ronchi, Paolo
Carl, Jil
Mizzon, Giulia
Martínez Serra, Pedro
Benvenuto, Giovanna
Arnone, Maria Ina
author Paganos, Periklis
author_facet Paganos, Periklis
Ronchi, Paolo
Carl, Jil
Mizzon, Giulia
Martínez Serra, Pedro
Benvenuto, Giovanna
Arnone, Maria Ina
author_role author
author2 Ronchi, Paolo
Carl, Jil
Mizzon, Giulia
Martínez Serra, Pedro
Benvenuto, Giovanna
Arnone, Maria Ina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Genètica molecular
Morfogènesi
Regulació genètica
Pàncrees
Molecular genetics
Morphogenesis
Genetic regulation
Pancreas
topic Genètica molecular
Morfogènesi
Regulació genètica
Pàncrees
Molecular genetics
Morphogenesis
Genetic regulation
Pancreas
description The identity and function of a given cell type relies on the differential expression of gene batteries that promote diverse phenotypes and functional specificities. Therefore, the identification of the molecular and morphological fingerprints of cell types across taxa is essential for untangling their evolution. Here we use a multidisciplinary approach to identify the molecular and morphological features of an exocrine, pancreas-like cell type harbored within the sea urchin larval gut. Using single cell transcriptomics, we identify various cell populations with a pancreatic-like molecular fingerprint that are enriched within the S. purpuratus larva digestive tract. Among these, in the region where they reside, the midgut/stomach domain, we find that populations of exocrine pancreas-like cells have a unique regulatory wiring distinct from the rest the of the cell types of the same region. Furthermore, Serial Block-face scanning Electron Microscopy (SBEM) of the exocrine cells shows that this reported molecular diversity is associated to distinct morphological features that reflect the physiological and functional properties of this cell type. Therefore, we propose that these sea urchin exocrine cells are homologous to the well-known mammalian pancreatic acinar cells and thus we trace the origin of this particular cell type to the time of deuterostome diversification. Overall, our approach allows a thorough characterization of a complex cell type and shows how both the transcriptomic and morphological information contribute to disentangling the evolution of cell types and organs such as the pancreatic cells and pancreas. Keywords: SBEM; acinar cells; evolution of cell types; morphology; pancreas; scRNAseq; sea urchin.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/192423
url https://hdl.handle.net/2445/192423
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fcell.2022.991664
Frontiers In Cell And Developmental Biology, 2022, vol. 10:991664
https://doi.org/10.3389/fcell.2022.991664
dc.rights.none.fl_str_mv cc-by (c) Paganos, Periklis et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Paganos, Periklis et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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