A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1

The gene underlying the melon fruit shape QTL fsqs8.1 is a member of the Ovate Family Proteins. Variation in fruit morphology is caused by changes in gene expression likely due to a cryptic structural variation in this locus. Melon cultivars have a wide range of fruit morphologies. Quantitative trai...

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Autores: Martínez-Martínez, Cecilia, Gonzalo Pascual, María José, Sipowicz, Pablo, Campos, Manuel, Martínez-Fernández, Irene, Leida, Carmen, Zouine, Mohammed, Alexiou, Konstantinos G., García-Mas, Jordi, Gómez, María Dolores, Tornero, Pablo, Pérez-Amador, Miguel A., Esteras, Cristina, Picó, Belén, Romero, Carlos, Monforte, Antonio J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/265825
Acceso en línea:http://hdl.handle.net/10261/265825
https://api.elsevier.com/content/abstract/scopus_id/85119887045
Access Level:acceso abierto
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spelling A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1Martínez-Martínez, CeciliaGonzalo Pascual, María JoséSipowicz, PabloCampos, ManuelMartínez-Fernández, IreneLeida, CarmenZouine, MohammedAlexiou, Konstantinos G.García-Mas, JordiGómez, María DoloresTornero, PabloPérez-Amador, Miguel A.Esteras, CristinaPicó, BelénRomero, CarlosMonforte, Antonio J.The gene underlying the melon fruit shape QTL fsqs8.1 is a member of the Ovate Family Proteins. Variation in fruit morphology is caused by changes in gene expression likely due to a cryptic structural variation in this locus. Melon cultivars have a wide range of fruit morphologies. Quantitative trait loci (QTL) have been identified underlying such diversity. This research focuses on the fruit shape QTL fsqs8.1, previously detected in a cross between the accession PI 124112 (CALC, producing elongated fruit) and the cultivar 'Piel de Sapo' (PS, producing oval fruit). The CALC fsqs8.1 allele induced round fruit shape, being responsible for the transgressive segregation for this trait observed in that population. In fact, the introgression line CALC8-1, carrying the fsqs8.1 locus from CALC into the PS genetic background, produced perfect round fruit. Following a map-based cloning approach, we found that the gene underlying fsqs8.1 is a member of the Ovate Family Proteins (OFP), CmOFP13, likely a homologue of AtOFP1 and SlOFP20 from Arabidopsis thaliana and tomato, respectively. The induction of the round shape was due to the higher expression of the CALC allele at the early ovary development stage. The fsqs8.1 locus showed an important structural variation, being CmOFP13 surrounded by two deletions in the CALC genome. The deletions are present at very low frequency in melon germplasm. Deletions and single nucleotide polymorphisms in the fsqs8.1 locus could not be not associated with variation in fruit shape among different melon accessions, what indicates that other genetic factors should be involved to induce the CALC fsqs8.1 allele effects. Therefore, fsqs8.1 is an example of a cryptic variation that alters gene expression, likely due to structural variation, resulting in phenotypic changes in melon fruit morphology.Peer reviewedSpringer0000-0003-3461-3094Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/265825https://api.elsevier.com/content/abstract/scopus_id/85119887045reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésTAG. Theoretical and applied genetics. Theoretische und angewandte Genetikhttps://doi.org/10.1007/s00122-021-03998-6Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2658252026-05-22T06:33:51Z
dc.title.none.fl_str_mv A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
title A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
spellingShingle A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
Martínez-Martínez, Cecilia
title_short A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
title_full A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
title_fullStr A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
title_full_unstemmed A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
title_sort A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
dc.creator.none.fl_str_mv Martínez-Martínez, Cecilia
Gonzalo Pascual, María José
Sipowicz, Pablo
Campos, Manuel
Martínez-Fernández, Irene
Leida, Carmen
Zouine, Mohammed
Alexiou, Konstantinos G.
García-Mas, Jordi
Gómez, María Dolores
Tornero, Pablo
Pérez-Amador, Miguel A.
Esteras, Cristina
Picó, Belén
Romero, Carlos
Monforte, Antonio J.
author Martínez-Martínez, Cecilia
author_facet Martínez-Martínez, Cecilia
Gonzalo Pascual, María José
Sipowicz, Pablo
Campos, Manuel
Martínez-Fernández, Irene
Leida, Carmen
Zouine, Mohammed
Alexiou, Konstantinos G.
García-Mas, Jordi
Gómez, María Dolores
Tornero, Pablo
Pérez-Amador, Miguel A.
Esteras, Cristina
Picó, Belén
Romero, Carlos
Monforte, Antonio J.
author_role author
author2 Gonzalo Pascual, María José
Sipowicz, Pablo
Campos, Manuel
Martínez-Fernández, Irene
Leida, Carmen
Zouine, Mohammed
Alexiou, Konstantinos G.
García-Mas, Jordi
Gómez, María Dolores
Tornero, Pablo
Pérez-Amador, Miguel A.
Esteras, Cristina
Picó, Belén
Romero, Carlos
Monforte, Antonio J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv 0000-0003-3461-3094
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description The gene underlying the melon fruit shape QTL fsqs8.1 is a member of the Ovate Family Proteins. Variation in fruit morphology is caused by changes in gene expression likely due to a cryptic structural variation in this locus. Melon cultivars have a wide range of fruit morphologies. Quantitative trait loci (QTL) have been identified underlying such diversity. This research focuses on the fruit shape QTL fsqs8.1, previously detected in a cross between the accession PI 124112 (CALC, producing elongated fruit) and the cultivar 'Piel de Sapo' (PS, producing oval fruit). The CALC fsqs8.1 allele induced round fruit shape, being responsible for the transgressive segregation for this trait observed in that population. In fact, the introgression line CALC8-1, carrying the fsqs8.1 locus from CALC into the PS genetic background, produced perfect round fruit. Following a map-based cloning approach, we found that the gene underlying fsqs8.1 is a member of the Ovate Family Proteins (OFP), CmOFP13, likely a homologue of AtOFP1 and SlOFP20 from Arabidopsis thaliana and tomato, respectively. The induction of the round shape was due to the higher expression of the CALC allele at the early ovary development stage. The fsqs8.1 locus showed an important structural variation, being CmOFP13 surrounded by two deletions in the CALC genome. The deletions are present at very low frequency in melon germplasm. Deletions and single nucleotide polymorphisms in the fsqs8.1 locus could not be not associated with variation in fruit shape among different melon accessions, what indicates that other genetic factors should be involved to induce the CALC fsqs8.1 allele effects. Therefore, fsqs8.1 is an example of a cryptic variation that alters gene expression, likely due to structural variation, resulting in phenotypic changes in melon fruit morphology.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/265825
https://api.elsevier.com/content/abstract/scopus_id/85119887045
url http://hdl.handle.net/10261/265825
https://api.elsevier.com/content/abstract/scopus_id/85119887045
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
https://doi.org/10.1007/s00122-021-03998-6
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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