Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei

The bacterial microbiota from the whole gut of soldier and worker castes of the termite Reticulitermes grassei was isolated and studied. In addition, the 16S rDNA bacterial genes from gut DNA were PCR-amplified using Bacteria-selective primers, and the 16S rDNA amplicons subsequently cloned into Esc...

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Detalles Bibliográficos
Autores: Berlanga Herranz, Mercedes, Paster, Bruce J., Grandcolas, Philipe, Guerrero, Ricardo, 1943-
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/52751
Acceso en línea:https://hdl.handle.net/2445/52751
Access Level:acceso abierto
Palabra clave:Microbiologia
Bacteris
Malalties bacterianes
Agents antiinfecciosos
Tèrmits
Microbiology
Bacteria
Bacterial diseases
Anti-infective agents
Termites
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spelling Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grasseiBerlanga Herranz, MercedesPaster, Bruce J.Grandcolas, PhilipeGuerrero, Ricardo, 1943-MicrobiologiaBacterisMalalties bacterianesAgents antiinfecciososTèrmitsMicrobiologyBacteriaBacterial diseasesAnti-infective agentsTermitesThe bacterial microbiota from the whole gut of soldier and worker castes of the termite Reticulitermes grassei was isolated and studied. In addition, the 16S rDNA bacterial genes from gut DNA were PCR-amplified using Bacteria-selective primers, and the 16S rDNA amplicons subsequently cloned into Escherichia coli. Sequences of the cloned inserts were then used to determine closest relatives by comparison with published sequences and with sequences from our previous work. The clones were found to be affiliated with the phyla Spirochaetes, Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Synergistetes, Verrucomicrobia, and candidate phyla Termite Group 1 (TG1) and Termite Group 2 (TG2). No significant differences were observed with respect to the relative bacterial abundances between soldier and worker phylotypes. The phylotypes obtained in this study were compared with reported sequences from other termites, especially those of phylotypes related to Spirochaetes, Wolbachia (an Alphaproteobacteria), Actinobacteria, and TG1. Many of the clone phylotypes detected in soldiers grouped with those of workers. Moreover, clones CRgS91 (soldiers) and CRgW68 (workers), both affiliated with"Endomicrobia", were the same phylotype. Soldiers and workers also seemed to have similar relative protist abundances. Heterotrophic, poly-β-hydroxyalkanoate-accumulating bacteria were isolated from the gut of soldiers and shown to be affiliated with Actinobacteria and Gammaproteobacteria. We noted that Wolbachia was detected in soldiers but not in workers. Overall, the maintenance by soldiers and workers of comparable axial and radial redox gradients in the gut is consistent with the similarities in the prokaryotes and protists comprising their microbiota.Spanish Society for Microbiology (SEM) and Viguera Editores SL2014201420112014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/52751Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.2436/20.1501.01.138International Microbiology, 2011, vol. 14, p. 83-93http://dx.doi.org/10.2436/20.1501.01.138cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011http://creativecommons.org/licenses/by-nc-sa/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/527512026-05-29T05:05:01Z
dc.title.none.fl_str_mv Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
title Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
spellingShingle Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
Berlanga Herranz, Mercedes
Microbiologia
Bacteris
Malalties bacterianes
Agents antiinfecciosos
Tèrmits
Microbiology
Bacteria
Bacterial diseases
Anti-infective agents
Termites
title_short Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
title_full Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
title_fullStr Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
title_full_unstemmed Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
title_sort Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei
dc.creator.none.fl_str_mv Berlanga Herranz, Mercedes
Paster, Bruce J.
Grandcolas, Philipe
Guerrero, Ricardo, 1943-
author Berlanga Herranz, Mercedes
author_facet Berlanga Herranz, Mercedes
Paster, Bruce J.
Grandcolas, Philipe
Guerrero, Ricardo, 1943-
author_role author
author2 Paster, Bruce J.
Grandcolas, Philipe
Guerrero, Ricardo, 1943-
author2_role author
author
author
dc.subject.none.fl_str_mv Microbiologia
Bacteris
Malalties bacterianes
Agents antiinfecciosos
Tèrmits
Microbiology
Bacteria
Bacterial diseases
Anti-infective agents
Termites
topic Microbiologia
Bacteris
Malalties bacterianes
Agents antiinfecciosos
Tèrmits
Microbiology
Bacteria
Bacterial diseases
Anti-infective agents
Termites
description The bacterial microbiota from the whole gut of soldier and worker castes of the termite Reticulitermes grassei was isolated and studied. In addition, the 16S rDNA bacterial genes from gut DNA were PCR-amplified using Bacteria-selective primers, and the 16S rDNA amplicons subsequently cloned into Escherichia coli. Sequences of the cloned inserts were then used to determine closest relatives by comparison with published sequences and with sequences from our previous work. The clones were found to be affiliated with the phyla Spirochaetes, Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Synergistetes, Verrucomicrobia, and candidate phyla Termite Group 1 (TG1) and Termite Group 2 (TG2). No significant differences were observed with respect to the relative bacterial abundances between soldier and worker phylotypes. The phylotypes obtained in this study were compared with reported sequences from other termites, especially those of phylotypes related to Spirochaetes, Wolbachia (an Alphaproteobacteria), Actinobacteria, and TG1. Many of the clone phylotypes detected in soldiers grouped with those of workers. Moreover, clones CRgS91 (soldiers) and CRgW68 (workers), both affiliated with"Endomicrobia", were the same phylotype. Soldiers and workers also seemed to have similar relative protist abundances. Heterotrophic, poly-β-hydroxyalkanoate-accumulating bacteria were isolated from the gut of soldiers and shown to be affiliated with Actinobacteria and Gammaproteobacteria. We noted that Wolbachia was detected in soldiers but not in workers. Overall, the maintenance by soldiers and workers of comparable axial and radial redox gradients in the gut is consistent with the similarities in the prokaryotes and protists comprising their microbiota.
publishDate 2011
dc.date.none.fl_str_mv 2011
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/52751
url https://hdl.handle.net/2445/52751
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.2436/20.1501.01.138
International Microbiology, 2011, vol. 14, p. 83-93
http://dx.doi.org/10.2436/20.1501.01.138
dc.rights.none.fl_str_mv cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
http://creativecommons.org/licenses/by-nc-sa/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
http://creativecommons.org/licenses/by-nc-sa/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Spanish Society for Microbiology (SEM) and Viguera Editores SL
publisher.none.fl_str_mv Spanish Society for Microbiology (SEM) and Viguera Editores SL
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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