The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference

The chromosome axis plays a crucial role in meiotic recombination. Here, we study the function of ASY1, the Arabidopsis homolog of the yeast chromosome axis-associated component Hop1. Specifically, we characterized cross-over (CO) distribution in female and male meiosis by deep sequencing of the pro...

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Autores: Pochon, Gaetan, Henry, Isabelle, Pradillo Orellana, Mónica, Yang, Chao, Lory, Niels, Fernández Jiménez, Nadia, Böwer, Franziska, Hu, Bingyan, Carstens, Lena, Tsai, Helen, Comai, Luca, Schnittger, Arp
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/98970
Acceso en línea:https://hdl.handle.net/20.500.14352/98970
Access Level:acceso abierto
Palabra clave:577.113
Genética
2409 Genética
2407.02 Citogenética
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spelling The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interferencePochon, GaetanHenry, IsabellePradillo Orellana, MónicaYang, ChaoLory, NielsFernández Jiménez, NadiaBöwer, FranziskaHu, BingyanCarstens, LenaTsai, HelenComai, LucaSchnittger, Arp577.113Genética2409 Genética2407.02 CitogenéticaThe chromosome axis plays a crucial role in meiotic recombination. Here, we study the function of ASY1, the Arabidopsis homolog of the yeast chromosome axis-associated component Hop1. Specifically, we characterized cross-over (CO) distribution in female and male meiosis by deep sequencing of the progeny of an allelic series of asy1 mutants. Combining data from nearly 1,000 individual plants, we find that reduced ASY1 functionality leads to genomic instability and sometimes drastic genomic rearrangements. We further observed that COs are less frequent and appear in more distal chromosomal regions in plants with no or reduced ASY1 functionality, consistent with previous analyses. However, our sequencing approach revealed that the reduction in CO number is not as dramatic as suggested by cytological analyses. Analysis of double mutants of asy1 with mutants with three other CO factors, MUS81, MSH4, and MSH5, as well as the determination of foci number of the CO regulator MLH1 demonstrates that the majority of the COs in asy1, similar to the situation in the wildtype (WT), largely belong to the class I, which are subject to interference. However, these COs are redistributed in asy1 mutants and typically appear much closer than in the WT. Hence, ASY1 plays a key role in CO interference that spaces COs along a chromosome. Conversely, since a large proportion of chromosomes do not receive any CO, we conclude that CO assurance, the process that ensures the obligatory assignment of one CO per chromosome, is also affected in asy1 mutants.Oxford AcademicUniversidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/98970reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/989702026-06-02T12:44:21Z
dc.title.none.fl_str_mv The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
title The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
spellingShingle The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
Pochon, Gaetan
577.113
Genética
2409 Genética
2407.02 Citogenética
title_short The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
title_full The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
title_fullStr The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
title_full_unstemmed The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
title_sort The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference
dc.creator.none.fl_str_mv Pochon, Gaetan
Henry, Isabelle
Pradillo Orellana, Mónica
Yang, Chao
Lory, Niels
Fernández Jiménez, Nadia
Böwer, Franziska
Hu, Bingyan
Carstens, Lena
Tsai, Helen
Comai, Luca
Schnittger, Arp
author Pochon, Gaetan
author_facet Pochon, Gaetan
Henry, Isabelle
Pradillo Orellana, Mónica
Yang, Chao
Lory, Niels
Fernández Jiménez, Nadia
Böwer, Franziska
Hu, Bingyan
Carstens, Lena
Tsai, Helen
Comai, Luca
Schnittger, Arp
author_role author
author2 Henry, Isabelle
Pradillo Orellana, Mónica
Yang, Chao
Lory, Niels
Fernández Jiménez, Nadia
Böwer, Franziska
Hu, Bingyan
Carstens, Lena
Tsai, Helen
Comai, Luca
Schnittger, Arp
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.113
Genética
2409 Genética
2407.02 Citogenética
topic 577.113
Genética
2409 Genética
2407.02 Citogenética
description The chromosome axis plays a crucial role in meiotic recombination. Here, we study the function of ASY1, the Arabidopsis homolog of the yeast chromosome axis-associated component Hop1. Specifically, we characterized cross-over (CO) distribution in female and male meiosis by deep sequencing of the progeny of an allelic series of asy1 mutants. Combining data from nearly 1,000 individual plants, we find that reduced ASY1 functionality leads to genomic instability and sometimes drastic genomic rearrangements. We further observed that COs are less frequent and appear in more distal chromosomal regions in plants with no or reduced ASY1 functionality, consistent with previous analyses. However, our sequencing approach revealed that the reduction in CO number is not as dramatic as suggested by cytological analyses. Analysis of double mutants of asy1 with mutants with three other CO factors, MUS81, MSH4, and MSH5, as well as the determination of foci number of the CO regulator MLH1 demonstrates that the majority of the COs in asy1, similar to the situation in the wildtype (WT), largely belong to the class I, which are subject to interference. However, these COs are redistributed in asy1 mutants and typically appear much closer than in the WT. Hence, ASY1 plays a key role in CO interference that spaces COs along a chromosome. Conversely, since a large proportion of chromosomes do not receive any CO, we conclude that CO assurance, the process that ensures the obligatory assignment of one CO per chromosome, is also affected in asy1 mutants.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/98970
url https://hdl.handle.net/20.500.14352/98970
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Academic
publisher.none.fl_str_mv Oxford Academic
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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