Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model

Fish skin mucus is composed of insoluble components, which form the physical barrier, and soluble components, which are key for interrelationship functions. Mucus is continuously secreted, but rates of production and exudation are still unknown, as are the underlying mechanisms. Using stable isotope...

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Autores: Ordóñez-Grande, Borja, Fernández-Alacid, Laura, Sanahuja Piera, Ignasi, Sánchez Nuño, Sergio, Fernández Borrás, J. (Jaume), Blasco Mínguez, Josefina, Ibarz i Valls, Antoni
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/181559
Acceso en línea:https://hdl.handle.net/2445/181559
Access Level:acceso abierto
Palabra clave:Moc (Fisiologia)
Orada
Mucus
Sparus aurata
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spelling Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish modelOrdóñez-Grande, BorjaFernández-Alacid, LauraSanahuja Piera, IgnasiSánchez Nuño, SergioFernández Borrás, J. (Jaume)Blasco Mínguez, JosefinaIbarz i Valls, AntoniMoc (Fisiologia)OradaMucusSparus aurataFish skin mucus is composed of insoluble components, which form the physical barrier, and soluble components, which are key for interrelationship functions. Mucus is continuously secreted, but rates of production and exudation are still unknown, as are the underlying mechanisms. Using stable isotope analysis, here, we evaluate skin mucus turnover and renewal in gilthead sea bream, separating raw mucus and its soluble and insoluble fractions. Isotopic abundance analysis reveals no differences between mucus and white muscle, thus confirming mucus samples as reliable non-invasive biomarkers. Mucus production was evaluated using a single labelled meal packaged in a gelatine capsule, with both 13C and 15N, via a time-course trial. 13C was gradually allocated to skin mucus fractions over the first 12 h and was significantly (4-fold) higher in the soluble fraction, indicating a higher turnover of soluble mucus components that are continuously produced and supplied. 15N was also gradually allocated to mucus, indicating incorporation of new proteins containing the labelled dietary amino acids, but with no differences between fractions. When existent mucus was removed, dietary stable isotopes revealed stimulated mucus neoformation dependent on the components. All this is novel knowledge concerning skin mucus dynamics and turnover in fish and could offer interesting non-invasive approaches to the use of skin mucus production in ecological or applied biological studies such as climate change effects, human impact, alterations in trophic networks or habitat degradation, especially of wild-captured species or protected species.Oxford University Press2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/181559Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1093/conphys/coaa095Conservation Physiology, 2020, vol. 8, num. 1, p. coaa095https://doi.org/10.1093/conphys/coaa095cc-by (c) Ordóñez-Grande, Borja et al., 2020https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1815592026-05-27T06:46:51Z
dc.title.none.fl_str_mv Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
title Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
spellingShingle Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
Ordóñez-Grande, Borja
Moc (Fisiologia)
Orada
Mucus
Sparus aurata
title_short Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
title_full Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
title_fullStr Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
title_full_unstemmed Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
title_sort Evaluating mucus exudation dynamics through isotopic enrichment and turnover of skin mucus fractions in a marine fish model
dc.creator.none.fl_str_mv Ordóñez-Grande, Borja
Fernández-Alacid, Laura
Sanahuja Piera, Ignasi
Sánchez Nuño, Sergio
Fernández Borrás, J. (Jaume)
Blasco Mínguez, Josefina
Ibarz i Valls, Antoni
author Ordóñez-Grande, Borja
author_facet Ordóñez-Grande, Borja
Fernández-Alacid, Laura
Sanahuja Piera, Ignasi
Sánchez Nuño, Sergio
Fernández Borrás, J. (Jaume)
Blasco Mínguez, Josefina
Ibarz i Valls, Antoni
author_role author
author2 Fernández-Alacid, Laura
Sanahuja Piera, Ignasi
Sánchez Nuño, Sergio
Fernández Borrás, J. (Jaume)
Blasco Mínguez, Josefina
Ibarz i Valls, Antoni
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Moc (Fisiologia)
Orada
Mucus
Sparus aurata
topic Moc (Fisiologia)
Orada
Mucus
Sparus aurata
description Fish skin mucus is composed of insoluble components, which form the physical barrier, and soluble components, which are key for interrelationship functions. Mucus is continuously secreted, but rates of production and exudation are still unknown, as are the underlying mechanisms. Using stable isotope analysis, here, we evaluate skin mucus turnover and renewal in gilthead sea bream, separating raw mucus and its soluble and insoluble fractions. Isotopic abundance analysis reveals no differences between mucus and white muscle, thus confirming mucus samples as reliable non-invasive biomarkers. Mucus production was evaluated using a single labelled meal packaged in a gelatine capsule, with both 13C and 15N, via a time-course trial. 13C was gradually allocated to skin mucus fractions over the first 12 h and was significantly (4-fold) higher in the soluble fraction, indicating a higher turnover of soluble mucus components that are continuously produced and supplied. 15N was also gradually allocated to mucus, indicating incorporation of new proteins containing the labelled dietary amino acids, but with no differences between fractions. When existent mucus was removed, dietary stable isotopes revealed stimulated mucus neoformation dependent on the components. All this is novel knowledge concerning skin mucus dynamics and turnover in fish and could offer interesting non-invasive approaches to the use of skin mucus production in ecological or applied biological studies such as climate change effects, human impact, alterations in trophic networks or habitat degradation, especially of wild-captured species or protected species.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/181559
url https://hdl.handle.net/2445/181559
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1093/conphys/coaa095
Conservation Physiology, 2020, vol. 8, num. 1, p. coaa095
https://doi.org/10.1093/conphys/coaa095
dc.rights.none.fl_str_mv cc-by (c) Ordóñez-Grande, Borja et al., 2020
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Ordóñez-Grande, Borja et al., 2020
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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