Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration

Photoreceptor loss is the principal cause of blindness in retinal degenerative diseases (RDDs). Whereas some therapies exist for early stages of RDDs, no effective treatment is currently available for later stages, and once photoreceptors are lost, the only option to rescue vision is cell transplant...

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Autores: Salas, Anna, Duarri, Anna, Fontrodona, Laura, Ramírez, Diana Mora, Badia, Anna, Isla Magrané, Helena, Ferreira de Souza, Barbara, Zapata, Miguel Ángel, Raya Chamorro, Ángel, Veiga, Anna, García Arumí, José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/176177
Acceso en línea:https://hdl.handle.net/2445/176177
Access Level:acceso abierto
Palabra clave:Teràpia cel·lular
Malalties de la retina
Ceguesa
Ratolins (Animals de laboratori)
Cellular therapy
Retinal diseases
Blindness
Mice (Laboratory animals)
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network_acronym_str ES
network_name_str España
repository_id_str
spelling Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degenerationSalas, AnnaDuarri, AnnaFontrodona, LauraRamírez, Diana MoraBadia, AnnaIsla Magrané, HelenaFerreira de Souza, BarbaraZapata, Miguel ÁngelRaya Chamorro, ÁngelVeiga, AnnaGarcía Arumí, JoséTeràpia cel·lularMalalties de la retinaCeguesaRatolins (Animals de laboratori)Cellular therapyRetinal diseasesBlindnessMice (Laboratory animals)Photoreceptor loss is the principal cause of blindness in retinal degenerative diseases (RDDs). Whereas some therapies exist for early stages of RDDs, no effective treatment is currently available for later stages, and once photoreceptors are lost, the only option to rescue vision is cell transplantation. With the use of the Royal College of Surgeons (RCS) rat model of retinal degeneration, we sought to determine whether combined transplantation of human-induced pluripotent stem cell (hiPSC)-derived retinal precursor cells (RPCs) and retinal pigment epithelial (RPE) cells was superior to RPE or RPC transplantation alone in preserving retinal from degeneration. hiPSC-derived RPCs and RPE cells expressing (GFP) were transplanted into the subretinal space of rats. In vivo monitoring showed that grafted cells survived 12 weeks in the subretinal space, and rats treated with RPE + RPC therapy exhibited better conservation of the outer nuclear layer (ONL) and visual response than RPE-treated or RPC-treated rats. Transplanted RPE cells integrated in the host RPE layer, whereas RPC mostly remained in the subretinal space, although a limited number of cells integrated in the ONL. In conclusion, the combined transplantation of hiPSC-derived RPE and RPCs is a potentially superior therapeutic approach to protect retina from degeneration in RDDs.Elsevier B. V.2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/176177Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.omtm.2021.02.006Molecular Therapy - Methods & Clinical Development, 2021, vol. 20, p. 688-702https://doi.org/10.1016/j.omtm.2021.02.006cc by-nc-nd (c) Salas et al., 2021http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1761772026-05-27T06:46:51Z
dc.title.none.fl_str_mv Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
title Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
spellingShingle Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
Salas, Anna
Teràpia cel·lular
Malalties de la retina
Ceguesa
Ratolins (Animals de laboratori)
Cellular therapy
Retinal diseases
Blindness
Mice (Laboratory animals)
title_short Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
title_full Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
title_fullStr Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
title_full_unstemmed Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
title_sort Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
dc.creator.none.fl_str_mv Salas, Anna
Duarri, Anna
Fontrodona, Laura
Ramírez, Diana Mora
Badia, Anna
Isla Magrané, Helena
Ferreira de Souza, Barbara
Zapata, Miguel Ángel
Raya Chamorro, Ángel
Veiga, Anna
García Arumí, José
author Salas, Anna
author_facet Salas, Anna
Duarri, Anna
Fontrodona, Laura
Ramírez, Diana Mora
Badia, Anna
Isla Magrané, Helena
Ferreira de Souza, Barbara
Zapata, Miguel Ángel
Raya Chamorro, Ángel
Veiga, Anna
García Arumí, José
author_role author
author2 Duarri, Anna
Fontrodona, Laura
Ramírez, Diana Mora
Badia, Anna
Isla Magrané, Helena
Ferreira de Souza, Barbara
Zapata, Miguel Ángel
Raya Chamorro, Ángel
Veiga, Anna
García Arumí, José
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Teràpia cel·lular
Malalties de la retina
Ceguesa
Ratolins (Animals de laboratori)
Cellular therapy
Retinal diseases
Blindness
Mice (Laboratory animals)
topic Teràpia cel·lular
Malalties de la retina
Ceguesa
Ratolins (Animals de laboratori)
Cellular therapy
Retinal diseases
Blindness
Mice (Laboratory animals)
description Photoreceptor loss is the principal cause of blindness in retinal degenerative diseases (RDDs). Whereas some therapies exist for early stages of RDDs, no effective treatment is currently available for later stages, and once photoreceptors are lost, the only option to rescue vision is cell transplantation. With the use of the Royal College of Surgeons (RCS) rat model of retinal degeneration, we sought to determine whether combined transplantation of human-induced pluripotent stem cell (hiPSC)-derived retinal precursor cells (RPCs) and retinal pigment epithelial (RPE) cells was superior to RPE or RPC transplantation alone in preserving retinal from degeneration. hiPSC-derived RPCs and RPE cells expressing (GFP) were transplanted into the subretinal space of rats. In vivo monitoring showed that grafted cells survived 12 weeks in the subretinal space, and rats treated with RPE + RPC therapy exhibited better conservation of the outer nuclear layer (ONL) and visual response than RPE-treated or RPC-treated rats. Transplanted RPE cells integrated in the host RPE layer, whereas RPC mostly remained in the subretinal space, although a limited number of cells integrated in the ONL. In conclusion, the combined transplantation of hiPSC-derived RPE and RPCs is a potentially superior therapeutic approach to protect retina from degeneration in RDDs.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176177
url https://hdl.handle.net/2445/176177
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.omtm.2021.02.006
Molecular Therapy - Methods & Clinical Development, 2021, vol. 20, p. 688-702
https://doi.org/10.1016/j.omtm.2021.02.006
dc.rights.none.fl_str_mv cc by-nc-nd (c) Salas et al., 2021
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc-nd (c) Salas et al., 2021
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B. V.
publisher.none.fl_str_mv Elsevier B. V.
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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