The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder

Berry Shrivel (BS) is a post-veraison physiological ripening disorder of grapevine berries. Its symptoms encompass low pH, reduced content of sugars and anthocyanins, and loss of turgor leading to berries shriveling. Evidence for the primary causes of BS is still speculative and anatomical studies a...

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Autores: Crespo Martínez, Sara, Sobczak, Miroslaw, Różańska, Elżbieta, Forneck, Astrid, Griesser, Michaela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/43825
Acceso en línea:https://hdl.handle.net/2454/43825
Access Level:acceso abierto
Palabra clave:Berry Shrivel
Phloem
Sieve element
Sieve tube conductivity
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spelling The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorderCrespo Martínez, SaraSobczak, MiroslawRóżańska, ElżbietaForneck, AstridGriesser, MichaelaBerry ShrivelPhloemSieve elementSieve tube conductivityBerry Shrivel (BS) is a post-veraison physiological ripening disorder of grapevine berries. Its symptoms encompass low pH, reduced content of sugars and anthocyanins, and loss of turgor leading to berries shriveling. Evidence for the primary causes of BS is still speculative and anatomical studies are scarce. So far, anatomical studies have determined necrotic cells, degraded primary phloem cells and hardening of secondary phloem cells in the rachis of BS affected grapes. The picture is far from being complete. Herein we report in-depth analyses of the ultrastructure, anatomy and spatial elementary analysis of rachis and pedicel tissues of BS symptomatic grape clusters with different symptom severity. We hypothesize that structural changes in the vascular system of BS affected grape clusters could alter transport functions of the phloem tissue and contribute to the appearance of BS symptoms. By applying different microscopic techniques (LM, SEM, TEM and EDS) we found a number of anatomical differences in both, rachis and pedicels, between H and BS symptomatic grapes, which include: (i) extended areas of collapsed cells and cell wall thickenings in the secondary phloem in BS samples; (ii) reduced number of cell layers in the cambium in BS samples; (iii) higher rate of callose deposition on sieve plates that are additionally covered with a carbohydrate-like material in BS samples; and (iv) reduced (up to 60%) estimated sieve tube conductivity in BS samples.The project was supported by the bilateral exchange and cooperation program between Austria and Poland (WTZ) project no. PL 08/2015 (9196) and by the Austrian Science Fund (FWF) project no. P 28966-B29.Agronomía, Biotecnología y AlimentaciónAgronomia, Bioteknologia eta Elikadura2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/mswordhttps://hdl.handle.net/2454/43825reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2018 The Authors. This is an open access article under the CC BY licensehttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/438252026-06-17T12:41:47Z
dc.title.none.fl_str_mv The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
title The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
spellingShingle The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
Crespo Martínez, Sara
Berry Shrivel
Phloem
Sieve element
Sieve tube conductivity
title_short The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
title_full The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
title_fullStr The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
title_full_unstemmed The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
title_sort The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder
dc.creator.none.fl_str_mv Crespo Martínez, Sara
Sobczak, Miroslaw
Różańska, Elżbieta
Forneck, Astrid
Griesser, Michaela
author Crespo Martínez, Sara
author_facet Crespo Martínez, Sara
Sobczak, Miroslaw
Różańska, Elżbieta
Forneck, Astrid
Griesser, Michaela
author_role author
author2 Sobczak, Miroslaw
Różańska, Elżbieta
Forneck, Astrid
Griesser, Michaela
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agronomía, Biotecnología y Alimentación
Agronomia, Bioteknologia eta Elikadura
dc.subject.none.fl_str_mv Berry Shrivel
Phloem
Sieve element
Sieve tube conductivity
topic Berry Shrivel
Phloem
Sieve element
Sieve tube conductivity
description Berry Shrivel (BS) is a post-veraison physiological ripening disorder of grapevine berries. Its symptoms encompass low pH, reduced content of sugars and anthocyanins, and loss of turgor leading to berries shriveling. Evidence for the primary causes of BS is still speculative and anatomical studies are scarce. So far, anatomical studies have determined necrotic cells, degraded primary phloem cells and hardening of secondary phloem cells in the rachis of BS affected grapes. The picture is far from being complete. Herein we report in-depth analyses of the ultrastructure, anatomy and spatial elementary analysis of rachis and pedicel tissues of BS symptomatic grape clusters with different symptom severity. We hypothesize that structural changes in the vascular system of BS affected grape clusters could alter transport functions of the phloem tissue and contribute to the appearance of BS symptoms. By applying different microscopic techniques (LM, SEM, TEM and EDS) we found a number of anatomical differences in both, rachis and pedicels, between H and BS symptomatic grapes, which include: (i) extended areas of collapsed cells and cell wall thickenings in the secondary phloem in BS samples; (ii) reduced number of cell layers in the cambium in BS samples; (iii) higher rate of callose deposition on sieve plates that are additionally covered with a carbohydrate-like material in BS samples; and (iv) reduced (up to 60%) estimated sieve tube conductivity in BS samples.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/43825
url https://hdl.handle.net/2454/43825
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2018 The Authors. This is an open access article under the CC BY license
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2018 The Authors. This is an open access article under the CC BY license
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/msword
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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