A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples

Microfluidic devices as sample treatment have showed significant advancements in recent years, becoming an important tool in pharmaceutical analysis. In this work, a green microfluidic method for the simultaneous extraction of basic polar and non-polar compounds is proposed for the first time, offer...

ver descrição completa

Detalhes bibliográficos
Autores: Martín, Alejandro, Santigosa, Elia, Ramos Payán, María Dolores
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2023
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/161990
Acesso em linha:https://hdl.handle.net/11441/161990
https://doi.org/10.1016/j.microc.2023.108553
Access Level:Acceso aberto
Palavra-chave:microfluidic
eutectic solvent
liquid phase microextraction
supported liquid membrane
pharmaceuticals
urine samples
id ES_bc75e0bd8ef93453dc65157aeb7efe4f
oai_identifier_str oai:idus.us.es:11441/161990
network_acronym_str ES
network_name_str España
repository_id_str
spelling A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samplesMartín, AlejandroSantigosa, EliaRamos Payán, María Doloresmicrofluidiceutectic solventliquid phase microextractionsupported liquid membranepharmaceuticalsurine samplesMicrofluidic devices as sample treatment have showed significant advancements in recent years, becoming an important tool in pharmaceutical analysis. In this work, a green microfluidic method for the simultaneous extraction of basic polar and non-polar compounds is proposed for the first time, offering a new and more sustainable and environmentally friendly alternative. A mixture of camphor:menthol 1:1 is presented as a new green supported liquid membrane (SLM) for the simultaneous extraction of a wide log P range for basic compounds (0.4 < log P < 5): verapamil hydrochloride (VRP), tyramine (TYR), atenolol (ATN), metopropol (MTP) and nortriptyline (NRP). Different SLMs were investigated to offer new other green alternatives for polar or non-polar basic drugs. Microfluidic operational parameters were also optimized at pH 1.5, pH 11.5 and 1 µL min−1 as acceptor phase, donor phase and donor flow rate, respectively. Camphor:menthol 1:1 showed good stability and reproducibility (RSD < 5%) in human urine samples. It was found to be an attractive alternative to traditional toxic organic solvents applied in microextraction techniques. The green microfluidic method offered good recoveries between 50 and 100% for all compounds in urine sample after 7 min extraction, using only 3 µL of Ca:Me 1:1 and showing a good long-term stability system up to 6 consecutive extraction in real samples. A relative standard deviation (n = 3) was below 6 % for all compounds with and without replacing the membrane of the system.Ministerio de Ciencia e Innovacion– Agencia Estatal de Investigacion PID2021-123073NB-C22ElsevierQuímica AnalíticaMinisterio de Ciencia e Innovación (MICIN). EspañaAgencia Estatal de Investigación. España2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/161990https://doi.org/10.1016/j.microc.2023.108553reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMicrochemical Journal, 189, 108553.PID2021-123073NB-C22https://dx.doi.org/10.1016/j.microc.2023.108553info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1619902026-06-17T12:51:07Z
dc.title.none.fl_str_mv A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
title A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
spellingShingle A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
Martín, Alejandro
microfluidic
eutectic solvent
liquid phase microextraction
supported liquid membrane
pharmaceuticals
urine samples
title_short A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
title_full A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
title_fullStr A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
title_full_unstemmed A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
title_sort A green microfluidic method for the simultaneous extraction of polar and non-polar basic compounds in biological samples
dc.creator.none.fl_str_mv Martín, Alejandro
Santigosa, Elia
Ramos Payán, María Dolores
author Martín, Alejandro
author_facet Martín, Alejandro
Santigosa, Elia
Ramos Payán, María Dolores
author_role author
author2 Santigosa, Elia
Ramos Payán, María Dolores
author2_role author
author
dc.contributor.none.fl_str_mv Química Analítica
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
dc.subject.none.fl_str_mv microfluidic
eutectic solvent
liquid phase microextraction
supported liquid membrane
pharmaceuticals
urine samples
topic microfluidic
eutectic solvent
liquid phase microextraction
supported liquid membrane
pharmaceuticals
urine samples
description Microfluidic devices as sample treatment have showed significant advancements in recent years, becoming an important tool in pharmaceutical analysis. In this work, a green microfluidic method for the simultaneous extraction of basic polar and non-polar compounds is proposed for the first time, offering a new and more sustainable and environmentally friendly alternative. A mixture of camphor:menthol 1:1 is presented as a new green supported liquid membrane (SLM) for the simultaneous extraction of a wide log P range for basic compounds (0.4 < log P < 5): verapamil hydrochloride (VRP), tyramine (TYR), atenolol (ATN), metopropol (MTP) and nortriptyline (NRP). Different SLMs were investigated to offer new other green alternatives for polar or non-polar basic drugs. Microfluidic operational parameters were also optimized at pH 1.5, pH 11.5 and 1 µL min−1 as acceptor phase, donor phase and donor flow rate, respectively. Camphor:menthol 1:1 showed good stability and reproducibility (RSD < 5%) in human urine samples. It was found to be an attractive alternative to traditional toxic organic solvents applied in microextraction techniques. The green microfluidic method offered good recoveries between 50 and 100% for all compounds in urine sample after 7 min extraction, using only 3 µL of Ca:Me 1:1 and showing a good long-term stability system up to 6 consecutive extraction in real samples. A relative standard deviation (n = 3) was below 6 % for all compounds with and without replacing the membrane of the system.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/161990
https://doi.org/10.1016/j.microc.2023.108553
url https://hdl.handle.net/11441/161990
https://doi.org/10.1016/j.microc.2023.108553
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microchemical Journal, 189, 108553.
PID2021-123073NB-C22
https://dx.doi.org/10.1016/j.microc.2023.108553
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418116197384192
score 15,811543

Registros relacionados