Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography

Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires...

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Detalles Bibliográficos
Autores: Sansen, T., Sánchez-Fuentes, D., Rathar, R., Colom-Diego, A., El Alaoui, F., Viaud, J., Macchione, M., De Rossi, S., Matile, Stefan, Gaudin, R., Bäcker, V., Carretero-Genevrier, A., Picas, L.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/338187
Acceso en línea:http://hdl.handle.net/10261/338187
https://api.elsevier.com/content/abstract/scopus_id/85087631327
Access Level:acceso abierto
Palabra clave:Biointerfaces
Cellular membranes
Microscopy
Nanostructured materials
Proteins
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spelling Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint LithographySansen, T.Sánchez-Fuentes, D.Rathar, R.Colom-Diego, A.El Alaoui, F.Viaud, J.Macchione, M.De Rossi, S.Matile, StefanGaudin, R.Bäcker, V.Carretero-Genevrier, A.Picas, L.BiointerfacesCellular membranesMicroscopyNanostructured materialsProteinsddc:540Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and benchtop method allowing one to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.L.P. acknowledges the ATIP-Avenir program for financial support. A.C.-G. acknowledges the financial support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (Grant No. 803004) and the French Agence Nationale de la Recherche (ANR), Project Q-NOSS ANR ANR-16-CE09-0006-01.Peer reviewedAmerican Chemical SocietyInstitut National de la Santé et de la Recherche Médicale (France)European CommissionAgence Nationale de la Recherche (France)Matile, Stefan [0000-0002-8537-8349]Carretero-Genevrier, A. [0000-0003-0488-9452]Picas, L. [0000-0002-5619-5228]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/338187https://api.elsevier.com/content/abstract/scopus_id/85087631327reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/803004//https://doi.org/10.1021/acsami.0c05432Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3381872026-05-22T06:33:51Z
dc.title.none.fl_str_mv Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
title Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
spellingShingle Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
Sansen, T.
Biointerfaces
Cellular membranes
Microscopy
Nanostructured materials
Proteins
ddc:540
title_short Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
title_full Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
title_fullStr Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
title_full_unstemmed Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
title_sort Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography
dc.creator.none.fl_str_mv Sansen, T.
Sánchez-Fuentes, D.
Rathar, R.
Colom-Diego, A.
El Alaoui, F.
Viaud, J.
Macchione, M.
De Rossi, S.
Matile, Stefan
Gaudin, R.
Bäcker, V.
Carretero-Genevrier, A.
Picas, L.
author Sansen, T.
author_facet Sansen, T.
Sánchez-Fuentes, D.
Rathar, R.
Colom-Diego, A.
El Alaoui, F.
Viaud, J.
Macchione, M.
De Rossi, S.
Matile, Stefan
Gaudin, R.
Bäcker, V.
Carretero-Genevrier, A.
Picas, L.
author_role author
author2 Sánchez-Fuentes, D.
Rathar, R.
Colom-Diego, A.
El Alaoui, F.
Viaud, J.
Macchione, M.
De Rossi, S.
Matile, Stefan
Gaudin, R.
Bäcker, V.
Carretero-Genevrier, A.
Picas, L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut National de la Santé et de la Recherche Médicale (France)
European Commission
Agence Nationale de la Recherche (France)
Matile, Stefan [0000-0002-8537-8349]
Carretero-Genevrier, A. [0000-0003-0488-9452]
Picas, L. [0000-0002-5619-5228]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biointerfaces
Cellular membranes
Microscopy
Nanostructured materials
Proteins
ddc:540
topic Biointerfaces
Cellular membranes
Microscopy
Nanostructured materials
Proteins
ddc:540
description Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and benchtop method allowing one to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.
publishDate 2020
dc.date.none.fl_str_mv 2020
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/338187
https://api.elsevier.com/content/abstract/scopus_id/85087631327
url http://hdl.handle.net/10261/338187
https://api.elsevier.com/content/abstract/scopus_id/85087631327
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/803004//
https://doi.org/10.1021/acsami.0c05432
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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