Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis

Capillary electrophoresis was applied for the first time to determine soybean proteins in commercial soybean products. The most suitable conditions for the analysis of these products in less than 7 min were 0.05 M phosphate buffer (pH 8) with 1 M urea; detection wavelength, 254 nm; applied voltage,...

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Detalles Bibliográficos
Autores: García Ruiz, Carmen|||0000-0001-5925-3449, García López, María Concepción|||0000-0002-3383-6176, Torre Roldán, María Mercedes|||0000-0002-1245-3504, Marina Alegre, María Luisa|||0000-0002-5583-1624
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/1362
Acceso en línea:http://hdl.handle.net/10017/1362
https://dx.doi.org/10.1002/(SICI)1522-2683(19990701)20:10<2003::AID-ELPS2003>3.0.CO;2-1
Access Level:acceso abierto
Palabra clave:Capillary electrophoresis
Soybean proteins
Quantitation
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
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spelling Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresisGarcía Ruiz, Carmen|||0000-0001-5925-3449García López, María Concepción|||0000-0002-3383-6176Torre Roldán, María Mercedes|||0000-0002-1245-3504Marina Alegre, María Luisa|||0000-0002-5583-1624Capillary electrophoresisSoybean proteinsQuantitationCienciaQuímica analítica e industrialScienceChemistry, analytic and technicalCapillary electrophoresis was applied for the first time to determine soybean proteins in commercial soybean products. The most suitable conditions for the analysis of these products in less than 7 min were 0.05 M phosphate buffer (pH 8) with 1 M urea; detection wavelength, 254 nm; applied voltage, 20 kV; and temperature, 30o C. Quantitation of soybean proteins was achieved using referenced conditions by means of the method of standard additions, using as standard a soybean protein isolate. This method was validated and applied to the quantitation of soybean proteins in commercial products derived from soybean protein isolate and soybean seeds.Universidad de AlcaláComunidad de MadridJohn Wiley & Sons19991999-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/1362https://dx.doi.org/10.1002/(SICI)1522-2683(19990701)20:10<2003::AID-ELPS2003>3.0.CO;2-1reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengNot available Not available Not availableComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available Not availableComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/13622026-06-18T11:13:07Z
dc.title.none.fl_str_mv Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
title Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
spellingShingle Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
García Ruiz, Carmen|||0000-0001-5925-3449
Capillary electrophoresis
Soybean proteins
Quantitation
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
title_short Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
title_full Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
title_fullStr Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
title_full_unstemmed Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
title_sort Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
dc.creator.none.fl_str_mv García Ruiz, Carmen|||0000-0001-5925-3449
García López, María Concepción|||0000-0002-3383-6176
Torre Roldán, María Mercedes|||0000-0002-1245-3504
Marina Alegre, María Luisa|||0000-0002-5583-1624
author García Ruiz, Carmen|||0000-0001-5925-3449
author_facet García Ruiz, Carmen|||0000-0001-5925-3449
García López, María Concepción|||0000-0002-3383-6176
Torre Roldán, María Mercedes|||0000-0002-1245-3504
Marina Alegre, María Luisa|||0000-0002-5583-1624
author_role author
author2 García López, María Concepción|||0000-0002-3383-6176
Torre Roldán, María Mercedes|||0000-0002-1245-3504
Marina Alegre, María Luisa|||0000-0002-5583-1624
author2_role author
author
author
dc.subject.none.fl_str_mv Capillary electrophoresis
Soybean proteins
Quantitation
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
topic Capillary electrophoresis
Soybean proteins
Quantitation
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
description Capillary electrophoresis was applied for the first time to determine soybean proteins in commercial soybean products. The most suitable conditions for the analysis of these products in less than 7 min were 0.05 M phosphate buffer (pH 8) with 1 M urea; detection wavelength, 254 nm; applied voltage, 20 kV; and temperature, 30o C. Quantitation of soybean proteins was achieved using referenced conditions by means of the method of standard additions, using as standard a soybean protein isolate. This method was validated and applied to the quantitation of soybean proteins in commercial products derived from soybean protein isolate and soybean seeds.
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/1362
https://dx.doi.org/10.1002/(SICI)1522-2683(19990701)20:10<2003::AID-ELPS2003>3.0.CO;2-1
url http://hdl.handle.net/10017/1362
https://dx.doi.org/10.1002/(SICI)1522-2683(19990701)20:10<2003::AID-ELPS2003>3.0.CO;2-1
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Not available Not available Not available
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available Not available
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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