The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis

High irradiances may lead to photooxidative stress in plants, and non-photochemical quenching (NPQ) contributes to protection against excess excitation. One of the NPQ mechanisms, qE, involves thermal dissipation of the light energy captured. Importantly, plants need to tune down qE under light-limi...

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Autores: Naranjo Río-Miranda, Belén, Mignée, Clara, Krieger-Liszkay, Anja, Hornero Méndez, Dámaso, Gallardo Guerrero, Lourdes, Cejudo Fernández, Francisco Javier, Lindahl, Marika
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/130183
Acceso en línea:https://hdl.handle.net/11441/130183
https://doi.org/10.1111/pce.12652
Access Level:acceso abierto
Palabra clave:High-light acclimation
Oxidative stress
Photosynthesis
Redox signalling
Thioredoxin
Thylakoid
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spelling The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in ArabidopsisNaranjo Río-Miranda, BelénMignée, ClaraKrieger-Liszkay, AnjaHornero Méndez, DámasoGallardo Guerrero, LourdesCejudo Fernández, Francisco JavierLindahl, MarikaHigh-light acclimationOxidative stressPhotosynthesisRedox signallingThioredoxinThylakoidHigh irradiances may lead to photooxidative stress in plants, and non-photochemical quenching (NPQ) contributes to protection against excess excitation. One of the NPQ mechanisms, qE, involves thermal dissipation of the light energy captured. Importantly, plants need to tune down qE under light-limiting conditions for efficient utilization of the available quanta. Considering the possible redox control of responses to excess light implying enzymes, such as thioredoxins, we have studied the role of the NADPH thioredoxin reductase C (NTRC). Whereas Arabidopsis thaliana plants lacking NTRC tolerate high light intensities, these plants display drastically elevated qE, have larger trans-thylakoid ΔpH and have 10-fold higher zeaxanthin levels under low and medium light intensities, leading to extremely low linear electron transport rates. To test the impact of the high qE on plant growth, we generated an ntrc-psbs double-knockout mutant, which is devoid of qE. This double mutant grows faster than the ntrc mutant and has a higher chlorophyll content. The photosystem II activity is partially restored in the ntrc-psbs mutant, and linear electron transport rates under low and medium light intensities are twice as high as compared with plants lacking ntrc alone. These data uncover a new role for NTRC in the control of photosynthetic yield.Ministerio de Economía y Competitividad BIO2013-43556-PWiley-BlackwellBioquímica Vegetal y Biología Molecular2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/130183https://doi.org/10.1111/pce.12652reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPlant, Cell and Environment, 39 (4), 804-822.BIO2013-43556-Phttps://doi.org/10.1111/pce.12652info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1301832026-06-17T12:51:07Z
dc.title.none.fl_str_mv The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
title The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
spellingShingle The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
Naranjo Río-Miranda, Belén
High-light acclimation
Oxidative stress
Photosynthesis
Redox signalling
Thioredoxin
Thylakoid
title_short The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
title_full The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
title_fullStr The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
title_full_unstemmed The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
title_sort The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis
dc.creator.none.fl_str_mv Naranjo Río-Miranda, Belén
Mignée, Clara
Krieger-Liszkay, Anja
Hornero Méndez, Dámaso
Gallardo Guerrero, Lourdes
Cejudo Fernández, Francisco Javier
Lindahl, Marika
author Naranjo Río-Miranda, Belén
author_facet Naranjo Río-Miranda, Belén
Mignée, Clara
Krieger-Liszkay, Anja
Hornero Méndez, Dámaso
Gallardo Guerrero, Lourdes
Cejudo Fernández, Francisco Javier
Lindahl, Marika
author_role author
author2 Mignée, Clara
Krieger-Liszkay, Anja
Hornero Méndez, Dámaso
Gallardo Guerrero, Lourdes
Cejudo Fernández, Francisco Javier
Lindahl, Marika
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
dc.subject.none.fl_str_mv High-light acclimation
Oxidative stress
Photosynthesis
Redox signalling
Thioredoxin
Thylakoid
topic High-light acclimation
Oxidative stress
Photosynthesis
Redox signalling
Thioredoxin
Thylakoid
description High irradiances may lead to photooxidative stress in plants, and non-photochemical quenching (NPQ) contributes to protection against excess excitation. One of the NPQ mechanisms, qE, involves thermal dissipation of the light energy captured. Importantly, plants need to tune down qE under light-limiting conditions for efficient utilization of the available quanta. Considering the possible redox control of responses to excess light implying enzymes, such as thioredoxins, we have studied the role of the NADPH thioredoxin reductase C (NTRC). Whereas Arabidopsis thaliana plants lacking NTRC tolerate high light intensities, these plants display drastically elevated qE, have larger trans-thylakoid ΔpH and have 10-fold higher zeaxanthin levels under low and medium light intensities, leading to extremely low linear electron transport rates. To test the impact of the high qE on plant growth, we generated an ntrc-psbs double-knockout mutant, which is devoid of qE. This double mutant grows faster than the ntrc mutant and has a higher chlorophyll content. The photosystem II activity is partially restored in the ntrc-psbs mutant, and linear electron transport rates under low and medium light intensities are twice as high as compared with plants lacking ntrc alone. These data uncover a new role for NTRC in the control of photosynthetic yield.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/130183
https://doi.org/10.1111/pce.12652
url https://hdl.handle.net/11441/130183
https://doi.org/10.1111/pce.12652
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plant, Cell and Environment, 39 (4), 804-822.
BIO2013-43556-P
https://doi.org/10.1111/pce.12652
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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