Unmasking the physiology of mercury detoxifying bacteria from polluted sediments

12 pages, 5 figures, 2 tables, supplementary material https://doi.org/10.1016/j.jhazmat.2024.133685.-- Data availability: Data will be made available on request.-- © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/...

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Autores: Pereira-García, Carla, Hernández-del-Amo, Elena, Vigués, Núria, Rey-Velasco, Xavier, Rincón-Tomás, Blanca, Pérez-Cruz, Carla, Sanz-Sáez, Isabel, Hu, Haiyan, Bertilsson, Stefan, Pannier, Angela, Soltmann, Ulrich, Sánchez Fernández, Pablo, Acinas, Silvia G., Bravo, Andrea G., Alonso-Sáez, Laura, Sánchez, Olga
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/347406
Acceso en línea:http://hdl.handle.net/10261/347406
https://api.elsevier.com/content/abstract/scopus_id/85184614216
Access Level:acceso abierto
Palabra clave:mer operon
Continuous culture
Mercury
Polluted sediments
Pseudomonas sp
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Make cities and human settlements inclusive, safe, resilient and sustainable
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spelling Unmasking the physiology of mercury detoxifying bacteria from polluted sedimentsPereira-García, CarlaHernández-del-Amo, ElenaVigués, NúriaRey-Velasco, XavierRincón-Tomás, BlancaPérez-Cruz, CarlaSanz-Sáez, IsabelHu, HaiyanBertilsson, StefanPannier, AngelaSoltmann, UlrichSánchez Fernández, PabloAcinas, Silvia G.Bravo, Andrea G.Alonso-Sáez, LauraSánchez, Olgamer operonContinuous cultureMercuryPolluted sedimentsPseudomonas sphttp://metadata.un.org/sdg/6http://metadata.un.org/sdg/11http://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesEnsure availability and sustainable management of water and sanitation for allMake cities and human settlements inclusive, safe, resilient and sustainable12 pages, 5 figures, 2 tables, supplementary material https://doi.org/10.1016/j.jhazmat.2024.133685.-- Data availability: Data will be made available on request.-- © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Marine sediments polluted from anthropogenic activities can be major reservoirs of toxic mercury species. Some microorganisms in these environments have the capacity to detoxify these pollutants, by using the mer operon. In this study, we characterized microbial cultures isolated from polluted marine sediments growing under diverse environmental conditions of salinity, oxygen availability and mercury tolerance. Specific growth rates and percentage of mercury removal were measured in batch cultures for a selection of isolates. A culture affiliated with Pseudomonas putida (MERCC_1942), which contained a mer operon as well as other genes related to metal resistances, was selected as the best candidate for mercury elimination. In order to optimize mercury detoxification conditions for strain MERCC_1942 in continuous culture, three different dilution rates were tested in bioreactors until the cultures achieved steady state, and they were subsequently exposed to a mercury spike; after 24 h, strain MERCC_1942 removed up to 76% of the total mercury. Moreover, when adapted to high growth rates in bioreactors, this strain exhibited the highest specific mercury detoxification rates. Finally, an immobilization protocol using the sol-gel technology was optimized. These results highlight that some sediment bacteria show capacity to detoxify mercury and could be used for bioremediation applications.This work has been co-funded by the European Climate, Infrastructure and Environment Executive Agency (CINEA, EMFF-BlueEconomy program) under grant agreement 2018-863584, project MER-CLUB. Elena H. del Amo and Isabel Sanz-Sáez are Margarita Salas fellows from the Spanish Government (REQ2021-UdG and UAB). We thank Verónica Melgarejo and Mireia Núñez for their lab support during the experiments.This work is contributing to the ICM’s ‘Center of Excellence’ Severo Ochoa (CEX2019-000928-S)Peer reviewedElsevierEuropean CommissionAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Sanz-Sáez, Isabel [0000-0003-0233-7224]Sánchez Fernández, Pablo [0000-0003-2787-822X]Acinas, Silvia G. [0000-0002-3439-0428]Bravo, Andrea G. [0000-0002-8341-3462]Sánchez, Olga [0000-0001-8331-8214]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/347406https://api.elsevier.com/content/abstract/scopus_id/85184614216reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CEX2019-000928-SJournal of hazardous materialshttps://doi.org/10.1016/j.jhazmat.2024.133685Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3474062026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
title Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
spellingShingle Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
Pereira-García, Carla
mer operon
Continuous culture
Mercury
Polluted sediments
Pseudomonas sp
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Make cities and human settlements inclusive, safe, resilient and sustainable
title_short Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
title_full Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
title_fullStr Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
title_full_unstemmed Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
title_sort Unmasking the physiology of mercury detoxifying bacteria from polluted sediments
dc.creator.none.fl_str_mv Pereira-García, Carla
Hernández-del-Amo, Elena
Vigués, Núria
Rey-Velasco, Xavier
Rincón-Tomás, Blanca
Pérez-Cruz, Carla
Sanz-Sáez, Isabel
Hu, Haiyan
Bertilsson, Stefan
Pannier, Angela
Soltmann, Ulrich
Sánchez Fernández, Pablo
Acinas, Silvia G.
Bravo, Andrea G.
Alonso-Sáez, Laura
Sánchez, Olga
author Pereira-García, Carla
author_facet Pereira-García, Carla
Hernández-del-Amo, Elena
Vigués, Núria
Rey-Velasco, Xavier
Rincón-Tomás, Blanca
Pérez-Cruz, Carla
Sanz-Sáez, Isabel
Hu, Haiyan
Bertilsson, Stefan
Pannier, Angela
Soltmann, Ulrich
Sánchez Fernández, Pablo
Acinas, Silvia G.
Bravo, Andrea G.
Alonso-Sáez, Laura
Sánchez, Olga
author_role author
author2 Hernández-del-Amo, Elena
Vigués, Núria
Rey-Velasco, Xavier
Rincón-Tomás, Blanca
Pérez-Cruz, Carla
Sanz-Sáez, Isabel
Hu, Haiyan
Bertilsson, Stefan
Pannier, Angela
Soltmann, Ulrich
Sánchez Fernández, Pablo
Acinas, Silvia G.
Bravo, Andrea G.
Alonso-Sáez, Laura
Sánchez, Olga
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Sanz-Sáez, Isabel [0000-0003-0233-7224]
Sánchez Fernández, Pablo [0000-0003-2787-822X]
Acinas, Silvia G. [0000-0002-3439-0428]
Bravo, Andrea G. [0000-0002-8341-3462]
Sánchez, Olga [0000-0001-8331-8214]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv mer operon
Continuous culture
Mercury
Polluted sediments
Pseudomonas sp
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Make cities and human settlements inclusive, safe, resilient and sustainable
topic mer operon
Continuous culture
Mercury
Polluted sediments
Pseudomonas sp
http://metadata.un.org/sdg/6
http://metadata.un.org/sdg/11
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Ensure availability and sustainable management of water and sanitation for all
Make cities and human settlements inclusive, safe, resilient and sustainable
description 12 pages, 5 figures, 2 tables, supplementary material https://doi.org/10.1016/j.jhazmat.2024.133685.-- Data availability: Data will be made available on request.-- © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/347406
https://api.elsevier.com/content/abstract/scopus_id/85184614216
url http://hdl.handle.net/10261/347406
https://api.elsevier.com/content/abstract/scopus_id/85184614216
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CEX2019-000928-S
Journal of hazardous materials
https://doi.org/10.1016/j.jhazmat.2024.133685
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
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